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The development of low-cost, point-of-care testing is crucial for gonorrhea control, especially in regions with limited medical facilities. In this study, we integrated CRISPR\/Cas12a reaction with recombinase polymerase amplification (RPA) to provide a simple and adaptable molecular detection method for\u00a0<jats:italic>N. gonorrhoeae<\/jats:italic>. The RPA-Cas12a-based detection system developed in this study enables rapid detection of <jats:italic>N. gonorrhoeae<\/jats:italic> within 1\u00a0h without the use of specialized equipment. This method is highly specific for identifying <jats:italic>N. gonorrhoeae<\/jats:italic> without cross-reactivity with other prevalent pathogens. Furthermore, in the evaluation of 24 clinical samples, the detection system demonstrates a 100% concordance rate with traditional culture, which is being used clinically as a reference method. Overall, the RPA-Cas12a-based <jats:italic>N. gonorrhoeae<\/jats:italic> detection has the advantages of rapidity, portability, low-cost, no special equipment required, and strong operability, and has a high potential for application as a self-testing and point-of-care diagnosis, which is critical for the clinical management of gonorrhea in developing countries lacking medical equipment.<\/jats:p>\n                <jats:p><jats:bold>Graphical Abstract<\/jats:bold><\/jats:p>","DOI":"10.1186\/s13568-023-01554-7","type":"journal-article","created":{"date-parts":[[2023,5,27]],"date-time":"2023-05-27T11:02:17Z","timestamp":1685185337000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Point-of-care detection of Neisseria gonorrhoeae based on RPA-CRISPR\/Cas12a"],"prefix":"10.1186","volume":"13","author":[{"given":"Qianrong","family":"Tu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoying","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Ling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Xiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7641-8823","authenticated-orcid":false,"given":"Shifeng","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,5,27]]},"reference":[{"issue":"6387","key":"1554_CR1","doi-asserted-by":"publisher","first-page":"436","DOI":"10.1126\/science.aar6245","volume":"360","author":"JS Chen","year":"2018","unstructured":"Chen JS, Ma E, Harrington LB, Da Costa M, Tian X, Palefsky JM, Doudna JA (2018) CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity. 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Isolates and clinical samples were collected at the microbiology laboratory of the hospital, with no contact with the patients. Therefore, the Ethics Review Board determined that informed consent was not required.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"All authors agree to be published.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declared no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"50"}}