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Existing anti-leishmanial treatments are not effective for a long time and associated with toxic side effects so searching for a new, effective and safe alternative treatments against infectious diseases is greatly needed. This study is aimed to assess the leishmaniacidal effects of methanolic extracts of <jats:italic>Eryngium planum<\/jats:italic> (<jats:italic>E. planum<\/jats:italic>) and <jats:italic>Ecbilliun elaterum<\/jats:italic> (<jats:italic>E. elaterum<\/jats:italic>) on <jats:italic>Leishmania major<\/jats:italic> (<jats:italic>L. major<\/jats:italic>), In vitro. The selected plants were collected from northern areas of Iran. The methanolic extract from the aerial parts of plants were prepared using maceration methods. GC- Mass analysis was used to determine the compounds of the plants. Promastigotes of <jats:italic>L. major<\/jats:italic> was cultured in RPMI-1640 medium and the anti-leishmanial and cytotoxicity effects of extracts at concentrations of 100, 200, 400 and 800\u00a0\u00b5g\/ml were assessed using MTT assay. The data obtained from gas chromatography revealed that \u03b1-Pinene, Caryophyllene oxide, \u03b2\u2010Caryophyllene, Bicyclogermacrene and \u03b1\u2010Bisabolol are the main compounds extracted from <jats:italic>E. planum<\/jats:italic> and \u03b1\u2010Pinene, Germacrene D, Caryophyllene oxide, \u03b3\u2010Eudesmol and \u03b1\u2010Bisabolol are the main components of <jats:italic>E. elaterum<\/jats:italic>. The results of MTT Assay revealed that <jats:italic>E. planum<\/jats:italic> at concentrations of 800\u00a0\u00b5g\/ml after 24\u00a0h at 400\u00a0\u00b5g\/ml after 48\u00a0h and the <jats:italic>E. elaterium<\/jats:italic> at concentrations of 800\u00a0\u00b5g\/ml after 48\u00a0h at 400\u00a0\u00b5g\/ml after 72\u00a0h had similar anti-leishmanial effects to the positive control. These results indicated that <jats:italic>E. planum<\/jats:italic> and <jats:italic>E. elaterum<\/jats:italic> are the potential sources for the discovery of novel anti-leishmanial treatments.<\/jats:p>","DOI":"10.1186\/s13568-023-01656-2","type":"journal-article","created":{"date-parts":[[2024,1,3]],"date-time":"2024-01-03T16:03:27Z","timestamp":1704297807000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Anti-leishmanial effects of Eryngium planum and Ecbilliun elaterum methanolic extract against Leishmania major"],"prefix":"10.1186","volume":"14","author":[{"given":"Erfan","family":"Ghaderian","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2595-9907","authenticated-orcid":false,"given":"Bahman Rahimi","family":"Esboei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parisa","family":"Mousavi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryam","family":"Pourhajibagher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Mohsen","family":"Homayouni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Zeinali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,1,3]]},"reference":[{"key":"1656_CR1","unstructured":"Abed AY (2009) Cytotoxicity of Fumaria officinalis on Leishmania tropica, l20b cell lines and bacteria in vitro. 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All methods were carried out in in adherence to international and guidelines for ethical conduct in the care and use of animals. The study was carried out in compliance with the ARRIVE guidelines.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}},{"value":"All authors of this article agree to submit the article to AMB Express Journal.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors of declare that there is no conflict of interest.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"3"}}