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The study particularly examined autolysis, indirectly associated with antibacterial resistance, when using <jats:italic>Coptis rhizome<\/jats:italic> for bacterial infections. In our methodology, <jats:italic>Coptis rhizome<\/jats:italic> was processed with ethanol and distilled water to produce four different extracts: CRET30, CRET50, CRET70, and CRDW. The antibacterial activity of these extracts were tested through Minimum Inhibitory Concentration (MIC) assays, disk diffusion tests, and time-kill assays, targeting both standard (ATCC 49619) and resistant (ATCC 70067) strains. The study also evaluated the extracts\u2019 biofilm inhibition properties and monitored the expression of the lyt gene, integral to autolysis. The results prominently showed that the CRET70 extract demonstrated remarkable antibacterial strength. It achieved an MIC of 0.125\u00a0\u00b5g\/mL against both tested <jats:italic>S. pneumoniae<\/jats:italic> strains. The disk diffusion assay recorded inhibition zones of 22.17\u00a0mm for ATCC 49619 and 17.20\u00a0mm for ATCC 70067. Impressively, CRET70 resulted in a 2-log decrease in bacterial numbers for both strains, showcasing its potent bactericidal capacity. The extract was also effective in inhibiting 77.40% of biofilm formation. Additionally, the significant overexpression of the lytA gene in the presence of CRET70 pointed to a potential mechanism of action for its antibacterial effects. The outcomes provided new perspectives on the use of <jats:italic>Coptis rhizome<\/jats:italic> in combating <jats:italic>S. pneumoniae<\/jats:italic>, especially significant in an era of escalating antibiotic resistance.<\/jats:p>","DOI":"10.1186\/s13568-024-01736-x","type":"journal-article","created":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T16:02:34Z","timestamp":1720108954000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Coptis rhizome extract influence on Streptococcus pneumoniae through autolysin activation"],"prefix":"10.1186","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8668-4508","authenticated-orcid":false,"given":"Eon-Bee","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1270-8554","authenticated-orcid":false,"given":"Kyubae","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,7,4]]},"reference":[{"issue":"2","key":"1736_CR1","doi-asserted-by":"publisher","first-page":"76","DOI":"10.7774\/cevr.2020.9.2.76","volume":"9","author":"D Afshar","year":"2020","unstructured":"Afshar D, Rafiee F, Kheirandish M, Moghadam SO, Azarsa M (2020) Autolysin (lytA) recombinant protein: a potential target for developing vaccines against pneumococcal infections. 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