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Therefore, this study aimed to evaluate an early diagnosis of cryptosporidiosis using the anti-<jats:italic>Cryptosporidium parvum<\/jats:italic> oocyst immunoglobulin IgG polyclonal antibodies (anti-<jats:italic>C. parvum<\/jats:italic> IgG PAbs)-based sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of <jats:italic>Cryptosporidium<\/jats:italic> oocyst antigens in fecal samples of farm animals in Egypt. Further molecular identification and sequencing were performed for the detected isolates. Eight hundred and twenty fecal samples of farm animals; 102 buffalo calves, 120 cattle calves, 100 lambs and 98 goat kids, 80 buffaloes, 60 cattle, 160 sheep and 100 goats, collected from different small-scale farms and local holders were examined for cryptosporidiosis by Modified Ziehl-Neelsen (MZN) technique. The percentage of positivity was 45.1%, 50%, 20%, 18.4%, 31.25%, 38.3%, 18.8%, and 11% in buffalo calves, cattle calves, lambs, goat kids, adult buffaloes, adult cattle, sheep, and goats, respectively. Molecular identification of <jats:italic>Cryptosporidium<\/jats:italic> samples was performed based on COWP gene, revealing the isolates: GenBank: OQ121955.1, OR029973.1 and PP316107.1 which were identical to the <jats:italic>C. parvum<\/jats:italic> and GenBank: PP316108.1 and OR029972.1 which were identical to <jats:italic>C. hominis<\/jats:italic> and <jats:italic>C. andersoni<\/jats:italic>, respectively. Then, <jats:italic>C. parvum<\/jats:italic> oocysts were used for preparation of antigens and rabbit immunization. Anti-<jats:italic>C. parvum<\/jats:italic> IgG PAbs were purified and characterized by SDS-PAGE and then labeled with horseradish peroxidase (HRP). Anti-<jats:italic>C. parvum<\/jats:italic> IgG PAbs in-house sandwich ELISA was prepared, then tested this ELISA on 820 samples and compared results with MZN microscopical examination and a commercial sandwich ELISA kit. In this study, in-house sandwich ELISA scored higher sensitivity of 98%, 100% specificity, validity 99% and relative agreement 98.6% than (92%, 90%, 91% and 91.4%) of MZN and (96%, 95%, 95.5% and 95.7%) of coproantigen commercial sandwich ELISA kit, respectively. Moreover, we used PCR to evaluate the positivity of in-house sandwich ELISA results, and the total PCR positive samples were 263 out of 268 sandwich ELISA positive samples (98.13%). <jats:bold>In conclusion<\/jats:bold>, the prepared Anti-<jats:italic>C. parvum<\/jats:italic> IgG PAbs based sandwich ELISA offered a simple and accurate diagnostic method for cryptosporidiosis in the fecal samples of different species of farm animals in Egypt with high sensitivity (98%) and specificity (100%). Further studies on this Anti-<jats:italic>C. parvum<\/jats:italic> IgG PAbs may help also in the protection against cryptosporidiosis.<\/jats:p>","DOI":"10.1186\/s13568-024-01817-x","type":"journal-article","created":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T18:54:27Z","timestamp":1737572067000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Coproantigen detection and molecular identification of Cryptosporidium species among newborn and adult farm animals"],"prefix":"10.1186","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2581-6503","authenticated-orcid":false,"given":"Dina","family":"Aboelsoued","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9718-5130","authenticated-orcid":false,"given":"Nagwa I.","family":"Toaleb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0723-7276","authenticated-orcid":false,"given":"Kadria N.","family":"Abdel Megeed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,1,22]]},"reference":[{"issue":"10","key":"1817_CR1","first-page":"342","volume":"8","author":"SM Abd El Hafez","year":"2010","unstructured":"Abd El Hafez SM, Anwar AM, Ibrahim AM, Mahmoud MB, Hassan HM (2010) Preparation of fluoresce isothiocyanate conjugated IgG (FITC) anti-camel and anti-buffalo. 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