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While over 350 species of this genus have been recorded, there has been no full investigation into whether different species may affect the allergen diagnosis and treatment. This study aimed to evaluate the variations in amino acid sequences and the content of major allergens, and how these affect specific IgE binding capacity in representative <jats:italic>Artemisia<\/jats:italic> species.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Methods<\/jats:title>\n<jats:p>Six representative <jats:italic>Artemisia<\/jats:italic> species from China and <jats:italic>Artemisia vulgaris<\/jats:italic> from Europe were used to determine allergen amino acid sequences by transcriptome, gene sequencing and mass spectrometry of the purified allergen component proteins. Sandwich ELISAs were developed and applied for Art v 1, Art v 2 and Art v 3 allergen quantification in different species. Aqueous pollen extracts and purified allergen components were used to assess IgE binding by ELISA and ImmunoCAP with mugwort allergic patient serum pools and individual sera from five areas in China.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>The Art v 1 and Art v 2 homologous allergen sequences in the seven <jats:italic>Artemisia<\/jats:italic> species were highly conserved. Art v 3 type allergens in <jats:italic>A. annua<\/jats:italic> and <jats:italic>A. sieversiana<\/jats:italic> were more divergent compared to <jats:italic>A. argyi<\/jats:italic> and <jats:italic>A. vulgaris<\/jats:italic>. The allergen content of Art v 1 group in the seven extracts ranged from 3.4% to 7.1%, that of Art v 2 from 1.0% to 3.6%, and Art v 3 from 0.3% to 10.5%. The highest IgE binding potency for most Chinese Artemisia allergy patients was with <jats:italic>A. annua<\/jats:italic> pollen extract, followed by <jats:italic>A. vulgaris<\/jats:italic> and <jats:italic>A. argyi<\/jats:italic>, with <jats:italic>A. sieversiana<\/jats:italic> significantly lower. Natural Art v 1-3 isoallergens from different species have almost equivalent IgE binding capacity in <jats:italic>Artemisia<\/jats:italic> allergic patients from China.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusion and clinical relevance<\/jats:title>\n<jats:p>There was high sequence similarity but different content of the three group allergens from different <jats:italic>Artemisia<\/jats:italic> species. Choice of <jats:italic>Artemisia annua<\/jats:italic> and <jats:italic>A. argyi<\/jats:italic> pollen source for diagnosis and immunotherapy is recommended in China.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s13601-020-00354-7","type":"journal-article","created":{"date-parts":[[2020,11,18]],"date-time":"2020-11-18T17:02:54Z","timestamp":1605718974000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Variation in IgE binding potencies of seven Artemisia species depending on content of major allergens"],"prefix":"10.1002","volume":"10","author":[{"given":"Lan","family":"Zhao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanyi","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biyuan","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shandong","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianqi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiying","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueyan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongtian","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianfei","family":"Lan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiling","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuefeng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuemei","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangmei","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriele","family":"Gadermaier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatima","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serge A.","family":"Versteeg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaap H.","family":"Akkerdaas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deyun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudolf","family":"Valenta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Vrtala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6944-9955","authenticated-orcid":false,"given":"Zhongshan","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald","family":"van Ree","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,11,18]]},"reference":[{"key":"354_CR1","doi-asserted-by":"publisher","first-page":"340","DOI":"10.1016\/S1081-1206(10)63025-6","volume":"79","author":"RK Katial","year":"1997","unstructured":"Katial RK, Lin FL, Stafford WW, Ledoux RA, Westley CR, Weber RW. 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Yu Huang Ding Hospital, Yantai, Shandong (authorization No. 2015-164), and Qvjing Chinese Traditional Medicine Hospital, Yunnan, China. The antibodies were produced by Hua An Biotech Ltd., Hangzhou, China (authorization No. SCXK 2016-0004 and SCXK 2017-0004).","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Gao ZS received a grant from Hangzhou Aileji Biotech Company, Hangzhou, China (Contract 2016R1). Gao ZS, Liu Y and Feng Y received funds from The Ministry of Science and Technology China (2019YFE0106600). Wang XY received a grant from Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support (ZYLX201826). Rudolf Valenta has received research grants from Viravaxx, Vienna, Austria and HVD Life Sciences, Vienna, Austria and serves as a consultant for Viravaxx. Susanne Vrtala received funds from the Austrian government (CN 14\/2020). A patent for Artemisia pollen allergen nsLTP quantification by ELISA has been applied in China (202011210352.1, pending) by ZS Gao, L\u00a0Zhao, BY Gao and WY Fu.\u00a0All other authors declare no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interest"}}],"article-number":"50"}}