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This manuscript analysed the efficacy of an alternative non-mandatory official control programme based on a multi-platform diagnostic panel and biosecurity developed and started in 185 herds placed in a contagious agalactia endemic area of Spain, using the data of 74,080 samples collected and analysed during a 4 years-period (2018\u20132021).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Globally, the combined analyses of bulk tank milk (BTM), ear or nasal swabs (in goats or sheep, respectively) and the serology to detect <jats:italic>Mycoplasma agalactiae<\/jats:italic> (<jats:italic>Ma<\/jats:italic>), allowed the detection of 40.54% of positive farms (<jats:italic>n<\/jats:italic>\u2009=\u200975), with <jats:italic>Ma<\/jats:italic> the species always detected in sheep (100%) and <jats:italic>Mycoplasma mycoides<\/jats:italic> subsp. <jats:italic>capri<\/jats:italic> (<jats:italic>Mmc<\/jats:italic>, 68,8%), <jats:italic>Ma<\/jats:italic> (29,3%) or both of them (1,9%) in goats. Taking into account productive aptitude and vaccination against CA, the use of BTM, ear or nasal swabs, and serology for herd classification demonstrated either a lack of concordance or only weak concordance. In herds that did not vaccinate, the classification of positives by male serology or swab detection showed moderate concordance. Vaccination against AC proved to be a protective factor against the occurrence of herds with bucks or rams testing positive.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>Since the different diagnostic techniques are not interchangeable, it is necessary to apply a multi-platform diagnostic panel for the accurate classification of herds. Based on official classification, strict biosecurity standards, including the prohibition of the entry of animals with unknown health status, allowed the completion of the CA control program.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/s13620-025-00288-1","type":"journal-article","created":{"date-parts":[[2025,1,18]],"date-time":"2025-01-18T05:23:01Z","timestamp":1737177781000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Multi-platform diagnostic strategy and biosecurity as basis of contagious agalactia control programs in endemic areas"],"prefix":"10.1186","volume":"78","author":[{"given":"Christian","family":"De la Fe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Grau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga","family":"Minguez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"S\u00e1nchez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosario","family":"Esquivel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan C.","family":"Corrales","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,1,18]]},"reference":[{"key":"288_CR1","doi-asserted-by":"publisher","first-page":"848","DOI":"10.20506\/rst.16.3.1062","volume":"16","author":"D Bergonier","year":"1997","unstructured":"Bergonier D, Berthelot X, Poumarat F. 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