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While human disease has been well characterized, animal data are dispersed across studies and lack a comprehensive global synthesis. This systematic review and meta-analysis estimated the pooled global prevalence of animal melioidosis and examined regional and diagnostic influences.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Following PRISMA 2020 guidelines, studies reporting\n                      <jats:italic>B. pseudomallei<\/jats:italic>\n                      detection in animals were retrieved from PubMed, Scopus, and Embase up to April 2025. Pooled prevalence was calculated using a random-effects model. Subgroup and meta-regression analyses explored moderators of heterogeneity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Twenty-eight studies from 11 countries, encompassing 98,885 animal samples, were included. The global pooled prevalence was 7.3% (95% CI: 0.039\u22120.117), with high heterogeneity (\n                      <jats:italic>I<\/jats:italic>\n                      \u00b2 = 98.8%). Southeast Asia, particularly Thailand, showed consistently higher prevalence across livestock, wildlife, and companion animals. Diagnostic methods and study period contributed to variability but were not statistically significant.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Animal melioidosis is globally distributed, with Thailand serving as a key endemic region and research hub. Strengthening One Health surveillance, standardizing diagnostic tools, and expanding genomic monitoring are essential for improved detection and control of\n                      <jats:italic>B. pseudomallei<\/jats:italic>\n                      across animal populations.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s13620-026-00339-1","type":"journal-article","created":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T05:46:11Z","timestamp":1774331171000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Global animal melioidosis prevalence: a systematic review and meta-analysis"],"prefix":"10.1186","volume":"79","author":[{"given":"Jongkonnee","family":"Thanasai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atthaphong","family":"Phongphithakchai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moragot","family":"Chatatikun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sa-ngob","family":"Laklaeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jitbanjong","family":"Tangpong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pakpoom","family":"Wongyikul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phichayut","family":"Phinyo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Supphachoke","family":"Khemla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anchalee","family":"Chittamma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wiyada Kwanhian","family":"Klangbud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,3,24]]},"reference":[{"key":"339_CR1","doi-asserted-by":"publisher","unstructured":"Chewapreecha C, Holden MT, Vehkala M, V\u00e4lim\u00e4ki N, Yang Z, Harris SR, et al. 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