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Effluent samples, heavily influenced by the chlorination had only 0.3 log<jats:sub>10<\/jats:sub> CFU\/mL of TEc. Fifty-one cefotaxime resistant isolates were selected out of an overall of 75 isolates, and subjected to a new round of testing, with a follow up of 36 and 48 isolates for both colistin and gentamicin, respectively as guided by initial results. Selected CREc exhibited resistance to amoxicillin-clavulanic acid (35.3%; <jats:italic>n<\/jats:italic>\u2009=\u200951), colistin sulphate (76.5%; <jats:italic>n<\/jats:italic>\u2009=\u200936), ciprofloxacin (47.1%; n\u2009=\u200951), gentamicin (87.5%; <jats:italic>n<\/jats:italic>\u2009=\u200948) and intermediate-resistance to meropenem (11.8%; n\u2009=\u200951). Extended spectrum-beta-lactamase genes detected, viz.: <jats:italic>bla<\/jats:italic><jats:sub><jats:italic>CTX<\/jats:italic>-M<\/jats:sub> (52.6%; <jats:italic>n<\/jats:italic>\u2009=\u200938) and <jats:italic>bla<\/jats:italic><jats:sub><jats:italic>TEM<\/jats:italic><\/jats:sub> (84.2%; n\u2009=\u200938) and concurrent <jats:italic>bla<\/jats:italic><jats:sub><jats:italic>CTX-M<\/jats:italic><\/jats:sub>\u00a0<jats:italic>+\u2009bla<\/jats:italic><jats:sub><jats:italic>TEM<\/jats:italic><\/jats:sub> (36.8%; n\u2009=\u200938), but no <jats:italic>bla<\/jats:italic><jats:sub><jats:italic>SHV<\/jats:italic><\/jats:sub> was detected. Carbapenem resistance genes, <jats:italic>blaKPC<\/jats:italic>-2 (15.8%; n\u2009=\u200938), <jats:italic>blaOXA<\/jats:italic>-1 (57.9%; n\u2009=\u200938), <jats:italic>blaNDM<\/jats:italic>-1 (15.8%; n\u2009=\u200938) were also detected. Approximately, 10.5 - 36.8% (n\u2009=\u200938) co-occurrence of two or more beta-lactamase genes was detected in some isolates. Out of the selected number (<jats:italic>n<\/jats:italic>\u2009=\u200930), 7(23.3%) were enterotoxigenic <jats:italic>E. coli<\/jats:italic> (ETEC), 14 (46.7%) were Enteroaggregative <jats:italic>E. coli<\/jats:italic> (EAEC), but no enteropathogenic <jats:italic>E. coli<\/jats:italic> (EPEC) was detected.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>Resistance to cefotaxime and the presence of a wide range of beta-lactamase genes exposed the potential risks associated with these pathogens via occupational and domestic exposure during the reuse of treated wastewater.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s13756-020-0702-4","type":"journal-article","created":{"date-parts":[[2020,3,12]],"date-time":"2020-03-12T14:02:50Z","timestamp":1584021770000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Antibiogram and beta-lactamase genes among cefotaxime resistant E. coli from wastewater treatment plant"],"prefix":"10.1186","volume":"9","author":[{"given":"Anthony Ayodeji","family":"Adegoke","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chibuzor Ezinne","family":"Madu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olayinka Ayobami","family":"Aiyegoro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thor Axel","family":"Stenstr\u00f6m","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony Ifeanyi","family":"Okoh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,3,12]]},"reference":[{"key":"702_CR1","doi-asserted-by":"publisher","first-page":"106S","DOI":"10.1111\/j.1365-2672.2000.tb05338.x","volume":"29","author":"SC Edberg","year":"2000","unstructured":"Edberg SC, Rice EW, Karlin RJ, Allen MJ. 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