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Therefore, we evaluated the AMR features and resistance mechanisms of the ESKAPEE pathogens collected in a tertiary hospital located in the capital of Malaysia.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>A total of 378 AMR-ESKAPEE strains were obtained based on convenience sampling over a nine-month study period (2019\u20132020). All strains were subjected to disk diffusion and broth microdilution assays to determine the antimicrobial susceptibility profiles. Polymerase chain reaction (PCR) and DNA sequence analyses were performed to determine the AMR genes profiles of the non-susceptible strains. Chi-square test and logistic regression analyses were used to correlate the AMR profiles and clinical data to determine the risk factors associated with HAIs.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>High rates of multidrug resistance (MDR) were observed in <jats:italic>A. baumannii<\/jats:italic>, <jats:italic>K. pneumoniae<\/jats:italic>, <jats:italic>E. coli<\/jats:italic>, and <jats:italic>S. aureus<\/jats:italic> (69\u201389%). All organisms except <jats:italic>E. coli<\/jats:italic> were frequently associated with HAIs (61\u201394%). Non-susceptibility to the last-resort drugs vancomycin (in <jats:italic>Enterococcus<\/jats:italic> spp. and <jats:italic>S. aureus<\/jats:italic>), carbapenems (in <jats:italic>A. baumannii<\/jats:italic>, <jats:italic>P. aeruginosa<\/jats:italic>, and <jats:italic>Enterobacteriaceae<\/jats:italic>), and colistin (in <jats:italic>Enterobacteriaceae<\/jats:italic>) were observed. Both <jats:italic>A. baumannii<\/jats:italic> and <jats:italic>K. pneumoniae<\/jats:italic> harbored a wide array of extended-spectrum \u03b2-lactamase genes (<jats:italic>bla<\/jats:italic><jats:sub>TEM<\/jats:sub>, <jats:italic>bla<\/jats:italic><jats:sub>SHV<\/jats:sub>, <jats:italic>bla<\/jats:italic><jats:sub>CTX-M<\/jats:sub>, <jats:italic>bla<\/jats:italic><jats:sub>OXA<\/jats:sub>). Metallo-\u03b2-lactamase genes (<jats:italic>bla<\/jats:italic><jats:sub>VEB<\/jats:sub>, <jats:italic>bla<\/jats:italic><jats:sub>VIM<\/jats:sub>, <jats:italic>bla<\/jats:italic><jats:sub>NDM<\/jats:sub>) were detected in carbapenem-resistant strains, at a higher frequency compared to other local reports. We detected two novel mutations in the quinolone-resistant determining region of the <jats:italic>gyr<\/jats:italic>A in fluoroquinolone-resistant <jats:italic>E. coli<\/jats:italic> (Leu-102-Ala; Gly-105-Val). Microbial resistance to ampicillin, methicillin, and cephalosporins was identified as important risk factors associated with HAIs in the hospital.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>Overall, our findings may provide valuable insight into the microbial resistance pattern and the risk factors of ESKAPEE-associated HAIs in a tertiary hospital located in central Peninsular Malaysia. The data obtained in this study may contribute to informing better hospital infection control in this region.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s13756-021-00936-5","type":"journal-article","created":{"date-parts":[[2021,4,23]],"date-time":"2021-04-23T10:04:14Z","timestamp":1619172254000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Genetic mechanisms and correlated risk factors of antimicrobial-resistant ESKAPEE pathogens isolated in a tertiary hospital in Malaysia"],"prefix":"10.1186","volume":"10","author":[{"given":"Soo Tein","family":"Ngoi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun Wie","family":"Chong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sasheela Sri La Sri","family":"Ponnampalavanar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soo Nee","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuryana","family":"Idris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kartini","family":"Abdul Jabar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Gregory","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tupur","family":"Husain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cindy Shuan Ju","family":"Teh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,4,23]]},"reference":[{"key":"936_CR1","volume-title":"Antimicrobial resistance: Global report on surveillance","author":"World Health Organization (WHO)","year":"2014","unstructured":"World Health Organization (WHO). 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