{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T21:31:15Z","timestamp":1791927075826,"version":"4.3.4"},"reference-count":56,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,2,14]],"date-time":"2025-02-14T00:00:00Z","timestamp":1739491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,2,14]],"date-time":"2025-02-14T00:00:00Z","timestamp":1739491200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82272547"],"award-info":[{"award-number":["82272547"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Special Project of Diagnosis and Treatment Technology for Key Clinical Diseases in Suzhou","award":["LCZX202203"],"award-info":[{"award-number":["LCZX202203"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Eur J Med Res"],"DOI":"10.1186\/s40001-025-02346-4","type":"journal-article","created":{"date-parts":[[2025,2,14]],"date-time":"2025-02-14T06:58:19Z","timestamp":1739516299000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Integrated bulk and single-cell RNA sequencing to identify potential biomarkers in intervertebral disc degeneration"],"prefix":"10.1186","volume":"30","author":[{"given":"Chunyang","family":"Fan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuefeng","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiale","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Hua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiexing","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiqing","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,2,14]]},"reference":[{"key":"2346_CR1","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1038\/s41584-021-00713-z","volume":"18","author":"V Francisco","year":"2022","unstructured":"Francisco V, Pino J, Gonz\u00e1lez-Gay M, Lago F, Karppinen J, Tervonen O, Mobasheri A, Gualillo O. A new immunometabolic perspective of intervertebral disc degeneration. Nat Rev Rheumatol. 2022;18:47\u201360.","journal-title":"Nat Rev Rheumatol"},{"key":"2346_CR2","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1038\/s41584-022-00888-z","volume":"19","author":"N Fine","year":"2023","unstructured":"Fine N, Lively S, S\u00e9guin CA, Perruccio AV, Kapoor M, Rampersaud R. Intervertebral disc degeneration and osteoarthritis: a common molecular disease spectrum. Nat Rev Rheumatol. 2023;19:136\u201352.","journal-title":"Nat Rev Rheumatol"},{"key":"2346_CR3","doi-asserted-by":"publisher","first-page":"2627","DOI":"10.1001\/jama.2016.16885","volume":"316","author":"JL Dieleman","year":"2016","unstructured":"Dieleman JL, Baral R, Birger M, Bui AL, Bulchis A, Chapin A, Hamavid H, Horst C, Johnson EK, Joseph J, Lavado R, Lomsadze L, Reynolds A, Squires E, Campbell M, DeCenso B, Dicker D, Flaxman AD, Gabert R, Highfill T, Naghavi M, Nightingale N, Templin T, Tobias MI, Vos T, Murray CJ. US spending on personal health care and public health, 1996\u20132013. JAMA. 2016;316:2627\u201346.","journal-title":"JAMA"},{"key":"2346_CR4","doi-asserted-by":"publisher","first-page":"398","DOI":"10.1016\/j.joca.2015.09.019","volume":"24","author":"F Wang","year":"2016","unstructured":"Wang F, Cai F, Shi R, Wang XH, Wu XT. Aging and age related stresses: a senescence mechanism of intervertebral disc degeneration. Osteoarthr Cartil. 2016;24:398\u2013408.","journal-title":"Osteoarthr Cartil"},{"key":"2346_CR5","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2022.101733","volume":"81","author":"K Sun","year":"2022","unstructured":"Sun K, Jiang J, Wang Y, Sun X, Zhu J, Xu X, Sun J, Shi J. The role of nerve fibers and their neurotransmitters in regulating intervertebral disc degeneration. Ageing Res Rev. 2022;81: 101733.","journal-title":"Ageing Res Rev"},{"key":"2346_CR6","doi-asserted-by":"publisher","first-page":"579","DOI":"10.1007\/s10495-012-0708-3","volume":"17","author":"F Ding","year":"2012","unstructured":"Ding F, Shao ZW, Yang SH, Wu Q, Gao F, Xiong LM. Role of mitochondrial pathway in compression-induced apoptosis of nucleus pulposus cells. Apoptosis. 2012;17:579\u201390.","journal-title":"Apoptosis"},{"key":"2346_CR7","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1038\/s41413-020-00125-x","volume":"9","author":"FJ Lyu","year":"2021","unstructured":"Lyu FJ, Cui H, Pan H, Mc Cheung K, Cao X, Iatridis JC, Zheng Z. Painful intervertebral disc degeneration and inflammation: from laboratory evidence to clinical interventions. Bone Res. 2021;9:7.","journal-title":"Bone Res"},{"key":"2346_CR8","doi-asserted-by":"publisher","first-page":"27","DOI":"10.7150\/thno.48987","volume":"11","author":"A Kamali","year":"2021","unstructured":"Kamali A, Ziadlou R, Lang G, Pfannkuche J, Cui S, Li Z, Richards RG, Alini M, Grad S. Small molecule-based treatment approaches for intervertebral disc degeneration: current options and future directions. Theranostics. 2021;11:27\u201347.","journal-title":"Theranostics"},{"key":"2346_CR9","doi-asserted-by":"publisher","first-page":"1373","DOI":"10.1111\/jcmm.13067","volume":"21","author":"Y Song","year":"2017","unstructured":"Song Y, Wang Y, Zhang Y, Geng W, Liu W, Gao Y, Li S, Wang K, Wu X, Kang L, Yang C. Advanced glycation end products regulate anabolic and catabolic activities via NLRP3-inflammasome activation in human nucleus pulposus cells. J Cell Mol Med. 2017;21:1373\u201387.","journal-title":"J Cell Mol Med"},{"key":"2346_CR10","doi-asserted-by":"publisher","DOI":"10.1002\/wdev.343","volume":"8","author":"S Mohanty","year":"2019","unstructured":"Mohanty S, Dahia CL. Defects in intervertebral disc and spine during development, degeneration, and pain: new research directions for disc regeneration and therapy. Wiley Interdiscip Rev Dev Biol. 2019;8: e343.","journal-title":"Wiley Interdiscip Rev Dev Biol"},{"key":"2346_CR11","doi-asserted-by":"publisher","first-page":"1715","DOI":"10.3390\/ijms23031715","volume":"23","author":"H Liang","year":"2022","unstructured":"Liang H, Luo R, Li G, Zhang W, Song Y, Yang C. The proteolysis of ECM in intervertebral disc degeneration. Int J Mol Sci. 2022;23:1715.","journal-title":"Int J Mol Sci"},{"key":"2346_CR12","doi-asserted-by":"publisher","first-page":"652","DOI":"10.1042\/BST0350652","volume":"35","author":"CL Le Maitre","year":"2007","unstructured":"Le Maitre CL, Pockert A, Buttle DJ, Freemont AJ, Hoyland JA. Matrix synthesis and degradation in human intervertebral disc degeneration. Biochem Soc Trans. 2007;35:652\u20135.","journal-title":"Biochem Soc Trans"},{"key":"2346_CR13","doi-asserted-by":"publisher","first-page":"1090467","DOI":"10.3389\/fgene.2022.1090467","volume":"13","author":"W Kuang","year":"2022","unstructured":"Kuang W, Jiang C, Yu C, Hu J, Duan Y, Chen Z. A microarray data analysis investigating the pathogenesis and potential biomarkers of autophagy and ferroptosis in intervertebral disc degeneration. Front Genet. 2022;13:1090467.","journal-title":"Front Genet"},{"key":"2346_CR14","doi-asserted-by":"publisher","first-page":"9020236","DOI":"10.1155\/2023\/9020236","volume":"2023","author":"J Guo","year":"2023","unstructured":"Guo J, Yang Y, Niu J, Luo Z, Shi Q, Yang H. Establishment of ferroptosis-related key gene signature and its validation in compression-induced intervertebral disc degeneration rats. Oxid Med Cell Longev. 2023;2023:9020236.","journal-title":"Oxid Med Cell Longev"},{"key":"2346_CR15","first-page":"9515571","volume":"2022","author":"N Wang","year":"2022","unstructured":"Wang N, Liu X, Fang X, Chen S, Xi Z, Zhang X, Xue C, Liu X, Xie L. Identification of SMIM1 and SEZ6L2 as potential biomarkers for genes associated with intervertebral disc degeneration in pyroptosis. Dis Markers. 2022;2022:9515571.","journal-title":"Dis Markers"},{"key":"2346_CR16","doi-asserted-by":"publisher","first-page":"3993","DOI":"10.3390\/ijms23073993","volume":"23","author":"H Cherif","year":"2022","unstructured":"Cherif H, Mannarino M, Pacis AS, Ragoussis J, Rabau O, Ouellet JA, Haglund L. Single-cell RNA-Seq analysis of cells from degenerating and non-degenerating intervertebral discs from the same individual reveals new biomarkers for intervertebral disc degeneration. Int J Mol Sci. 2022;23:3993.","journal-title":"Int J Mol Sci"},{"key":"2346_CR17","doi-asserted-by":"publisher","first-page":"1920","DOI":"10.1161\/CIRCULATIONAHA.115.001593","volume":"132","author":"RC Deo","year":"2015","unstructured":"Deo RC. Machine learning in medicine. Circulation. 2015;132:1920\u201330.","journal-title":"Circulation"},{"key":"2346_CR18","doi-asserted-by":"publisher","first-page":"603","DOI":"10.1111\/joim.12822","volume":"284","author":"GS Handelman","year":"2018","unstructured":"Handelman GS, Kok HK, Chandra RV, Razavi AH, Lee MJ, Asadi H. eDoctor: machine learning and the future of medicine. J Intern Med. 2018;284:603\u201319.","journal-title":"J Intern Med"},{"key":"2346_CR19","doi-asserted-by":"publisher","first-page":"7189854","DOI":"10.1155\/2019\/7189854","volume":"2019","author":"Y Liu","year":"2019","unstructured":"Liu Y, Lin J, Wu X, Guo X, Sun H, Yu B, Shen J, Bai J, Chen Z, Yang H, Geng D, Mao H. Aspirin-mediated attenuation of intervertebral disc degeneration by ameliorating reactive oxygen species in vivo and in vitro. Oxid Med Cell Longev. 2019;2019:7189854.","journal-title":"Oxid Med Cell Longev"},{"key":"2346_CR20","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1002\/jor.22533","volume":"32","author":"H Hirata","year":"2014","unstructured":"Hirata H, Yurube T, Kakutani K, Maeno K, Takada T, Yamamoto J, Kurakawa T, Akisue T, Kuroda R, Kurosaka M, Nishida K. A rat tail temporary static compression model reproduces different stages of intervertebral disc degeneration with decreased notochordal cell phenotype. J Orthopaed Res. 2014;32:455\u201363.","journal-title":"J Orthopaed Res"},{"key":"2346_CR21","doi-asserted-by":"publisher","first-page":"518","DOI":"10.1038\/s12276-022-00753-9","volume":"54","author":"S Chen","year":"2022","unstructured":"Chen S, Lei L, Li Z, Chen F, Huang Y, Jiang G, Guo X, Zhao Z, Liu H, Wang H, Liu C, Zheng Z, Wang J. Grem1 accelerates nucleus pulposus cell apoptosis and intervertebral disc degeneration by inhibiting TGF-\u03b2-mediated Smad2\/3 phosphorylation. Exp Mol Med. 2022;54:518\u201330.","journal-title":"Exp Mol Med"},{"key":"2346_CR22","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1016\/j.biomaterials.2017.03.013","volume":"129","author":"RD Bowles","year":"2017","unstructured":"Bowles RD, Setton LA. Biomaterials for intervertebral disc regeneration and repair. Biomaterials. 2017;129:54\u201367.","journal-title":"Biomaterials"},{"key":"2346_CR23","doi-asserted-by":"publisher","first-page":"90","DOI":"10.3389\/fbioe.2018.00090","volume":"6","author":"G Chu","year":"2018","unstructured":"Chu G, Shi C, Wang H, Zhang W, Yang H, Li B. Strategies for annulus fibrosus regeneration: from biological therapies to tissue engineering. Front Bioeng Biotechnol. 2018;6:90.","journal-title":"Front Bioeng Biotechnol"},{"key":"2346_CR24","doi-asserted-by":"publisher","first-page":"1469","DOI":"10.1038\/s41467-022-28990-6","volume":"13","author":"G Li","year":"2022","unstructured":"Li G, Ma L, He S, Luo R, Wang B, Zhang W, Song Y, Liao Z, Ke W, Xiang Q, Feng X, Wu X, Zhang Y, Wang K, Yang C. WTAP-mediated m(6)A modification of lncRNA NORAD promotes intervertebral disc degeneration. Nat Commun. 2022;13:1469.","journal-title":"Nat Commun"},{"key":"2346_CR25","doi-asserted-by":"publisher","first-page":"4084","DOI":"10.7150\/thno.33638","volume":"9","author":"Z Liao","year":"2019","unstructured":"Liao Z, Luo R, Li G, Song Y, Zhan S, Zhao K, Hua W, Zhang Y, Wu X, Yang C. Exosomes from mesenchymal stem cells modulate endoplasmic reticulum stress to protect against nucleus pulposus cell death and ameliorate intervertebral disc degeneration in vivo. Theranostics. 2019;9:4084\u2013100.","journal-title":"Theranostics"},{"key":"2346_CR26","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1016\/j.biomaterials.2018.01.016","volume":"160","author":"Z Pan","year":"2018","unstructured":"Pan Z, Sun H, Xie B, Xia D, Zhang X, Yu D, Li J, Xu Y, Wang Z, Wu Y, Zhang X, Wang Y, Fu Q, Hu W, Yang Y, Bunpetch V, Shen W, Heng BC, Zhang S, Ouyang H. Therapeutic effects of gefitinib-encapsulated thermosensitive injectable hydrogel in intervertebral disc degeneration. Biomaterials. 2018;160:56\u201368.","journal-title":"Biomaterials"},{"key":"2346_CR27","doi-asserted-by":"publisher","first-page":"7115","DOI":"10.1002\/jcp.27466","volume":"234","author":"XB Wang","year":"2019","unstructured":"Wang XB, Wang H, Long HQ, Li DY, Zheng X. LINC00641 regulates autophagy and intervertebral disc degeneration by acting as a competitive endogenous RNA of miR-153-3p under nutrition deprivation stress. J Cell Physiol. 2019;234:7115\u201327.","journal-title":"J Cell Physiol"},{"key":"2346_CR28","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2022.864100","volume":"13","author":"J Li","year":"2022","unstructured":"Li J, Yu C, Ni S, Duan Y. Identification of core genes and screening of potential targets in intervertebral disc degeneration using integrated bioinformatics analysis. Front Genet. 2022;13: 864100.","journal-title":"Front Genet"},{"key":"2346_CR29","doi-asserted-by":"publisher","first-page":"1419","DOI":"10.2217\/epi-2020-0101","volume":"12","author":"X Wang","year":"2020","unstructured":"Wang X, Chen N, Du Z, Ling Z, Zhang P, Yang J, Khaleel M, Khoury AN, Li J, Li S, Huang H, Zhou X, Han Y, Wei F. Bioinformatics analysis integrating metabolomics of m(6)A RNA microarray in intervertebral disc degeneration. Epigenomics. 2020;12:1419\u201341.","journal-title":"Epigenomics"},{"key":"2346_CR30","doi-asserted-by":"publisher","first-page":"1372483","DOI":"10.1155\/2022\/1372483","volume":"2022","author":"Z Zhu","year":"2022","unstructured":"Zhu Z, He Z, Tang T, Wang F, Chen H, Li B, Chen G, Wang J, Tian W, Chen D, Wu X, Liu X, Zhou Z, Liu S. Integrative bioinformatics analysis revealed mitochondrial dysfunction-related genes underlying intervertebral disc degeneration. Oxid Med Cell Longev. 2022;2022:1372483.","journal-title":"Oxid Med Cell Longev"},{"key":"2346_CR31","doi-asserted-by":"publisher","first-page":"1324","DOI":"10.1016\/j.joca.2021.06.010","volume":"29","author":"Y Zhang","year":"2021","unstructured":"Zhang Y, Han S, Kong M, Tu Q, Zhang L, Ma X. Single-cell RNA-seq analysis identifies unique chondrocyte subsets and reveals involvement of ferroptosis in human intervertebral disc degeneration. Osteoarthr Cartil. 2021;29:1324\u201334.","journal-title":"Osteoarthr Cartil"},{"key":"2346_CR32","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.833420","volume":"9","author":"Z Ling","year":"2021","unstructured":"Ling Z, Liu Y, Wang Z, Zhang Z, Chen B, Yang J, Zeng B, Gao Y, Jiang C, Huang Y, Zou X, Wang X, Wei F. Single-cell RNA-Seq analysis reveals macrophage involved in the progression of human intervertebral disc degeneration. Front Cell Dev Biol. 2021;9: 833420.","journal-title":"Front Cell Dev Biol"},{"key":"2346_CR33","doi-asserted-by":"publisher","first-page":"1242909","DOI":"10.3389\/fimmu.2023.1242909","volume":"14","author":"W Xu","year":"2023","unstructured":"Xu W, Zhang W, Zhao D, Wang Q, Zhang M, Li Q, Zhu W, Xu C. Unveiling the role of regulatory T cells in the tumor microenvironment of pancreatic cancer through single-cell transcriptomics and in vitro experiments. Front Immunol. 2023;14:1242909.","journal-title":"Front Immunol"},{"key":"2346_CR34","doi-asserted-by":"publisher","first-page":"1224832","DOI":"10.3389\/fendo.2023.1224832","volume":"14","author":"W Xu","year":"2023","unstructured":"Xu W, Jiang T, Shen K, Zhao D, Zhang M, Zhu W, Liu Y, Xu C. GADD45B regulates the carcinogenesis process of chronic atrophic gastritis and the metabolic pathways of gastric cancer. Front Endocrinol. 2023;14:1224832.","journal-title":"Front Endocrinol"},{"key":"2346_CR35","doi-asserted-by":"publisher","first-page":"828","DOI":"10.1109\/JBHI.2021.3094578","volume":"26","author":"Y Fu","year":"2022","unstructured":"Fu Y, Xue P, Ren M, Dong E. Harmony loss for unbalanced prediction. IEEE J Biomed Health Inform. 2022;26:828\u201339.","journal-title":"IEEE J Biomed Health Inform"},{"key":"2346_CR36","doi-asserted-by":"publisher","first-page":"496","DOI":"10.1038\/s41586-019-0969-x","volume":"566","author":"J Cao","year":"2019","unstructured":"Cao J, Spielmann M, Qiu X, Huang X, Ibrahim DM, Hill AJ, Zhang F, Mundlos S, Christiansen L, Steemers FJ, Trapnell C, Shendure J. The single-cell transcriptional landscape of mammalian organogenesis. Nature. 2019;566:496\u2013502.","journal-title":"Nature"},{"key":"2346_CR37","doi-asserted-by":"publisher","DOI":"10.1016\/j.crmeth.2023.100498","volume":"3","author":"S Morabito","year":"2023","unstructured":"Morabito S, Reese F, Rahimzadeh N, Miyoshi E, Swarup V. hdWGCNA identifies co-expression networks in high-dimensional transcriptomics data. Cell Rep Methods. 2023;3: 100498.","journal-title":"Cell Rep Methods"},{"key":"2346_CR38","doi-asserted-by":"publisher","first-page":"432","DOI":"10.1186\/s12859-018-2451-4","volume":"19","author":"H Sanz","year":"2018","unstructured":"Sanz H, Valim C, Vegas E, Oller JM, Reverter F. SVM-RFE: selection and visualization of the most relevant features through non-linear kernels. BMC Bioinform. 2018;19:432.","journal-title":"BMC Bioinform"},{"key":"2346_CR39","first-page":"31","volume":"47","author":"SJ Rigatti","year":"2017","unstructured":"Rigatti SJ. Random Forest. J Insur Med (New York, N Y). 2017;47:31\u20139.","journal-title":"J Insur Med (New York, N. Y.)"},{"key":"2346_CR40","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2020.107720","volume":"220","author":"J Huang","year":"2021","unstructured":"Huang J. Current developments of targeting the p53 signaling pathway for cancer treatment. Pharmacol Ther. 2021;220: 107720.","journal-title":"Pharmacol Ther"},{"key":"2346_CR41","doi-asserted-by":"publisher","DOI":"10.1111\/acel.12951","volume":"18","author":"L Chen","year":"2019","unstructured":"Chen L, Yang R, Qiao W, Zhang W, Chen J, Mao L, Goltzman D, Miao D. 1,25-Dihydroxyvitamin D exerts an antiaging role by activation of Nrf2-antioxidant signaling and inactivation of p16\/p53-senescence signaling. Aging Cell. 2019;18: e12951.","journal-title":"Aging Cell"},{"key":"2346_CR42","doi-asserted-by":"publisher","first-page":"5129","DOI":"10.1038\/onc.2012.640","volume":"32","author":"A Rufini","year":"2013","unstructured":"Rufini A, Tucci P, Celardo I, Melino G. Senescence and aging: the critical roles of p53. Oncogene. 2013;32:5129\u201343.","journal-title":"Oncogene"},{"key":"2346_CR43","doi-asserted-by":"publisher","DOI":"10.1111\/acel.13162","volume":"19","author":"Y Han","year":"2020","unstructured":"Han Y, Zhou CM, Shen H, Tan J, Dong Q, Zhang L, McGowan SJ, Zhao J, Sowa GA, Kang JD, Niedernhofer LJ, Robbins PD, Vo NN. Attenuation of ataxia telangiectasia mutated signalling mitigates age-associated intervertebral disc degeneration. Aging Cell. 2020;19: e13162.","journal-title":"Aging Cell"},{"key":"2346_CR44","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1186\/s12951-024-02556-8","volume":"22","author":"C Fan","year":"2024","unstructured":"Fan C, Wang W, Yu Z, Wang J, Xu W, Ji Z, He W, Hua D, Wang W, Yao L, Deng Y, Geng D, Wu X, Mao H. M1 macrophage-derived exosomes promote intervertebral disc degeneration by enhancing nucleus pulposus cell senescence through LCN2\/NF-\u03baB signaling axis. J Nanobiotechnology. 2024;22:301.","journal-title":"J Nanobiotechnology"},{"key":"2346_CR45","doi-asserted-by":"publisher","first-page":"766","DOI":"10.7150\/ijbs.40189","volume":"16","author":"J Ge","year":"2020","unstructured":"Ge J, Cheng X, Yuan C, Qian J, Wu C, Cao C, Yang H, Zhou F, Zou J. Syndecan-4 is a novel therapeutic target for intervertebral disc degeneration via suppressing JNK\/p53 pathway. Int J Biol Sci. 2020;16:766\u201376.","journal-title":"Int J Biol Sci"},{"key":"2346_CR46","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202206888","volume":"19","author":"Y Peng","year":"2023","unstructured":"Peng Y, Chen X, Liu S, Wu W, Shu H, Tian S, Xiao Y, Li K, Wang B, Lin H, Qing X, Shao Z. Extracellular vesicle-conjugated functional matrix hydrogels prevent senescence by exosomal miR-3594\u20135p-Targeted HIPK2\/p53 pathway for disc regeneration. Small. 2023;19: e2206888.","journal-title":"Small"},{"key":"2346_CR47","doi-asserted-by":"publisher","first-page":"2367","DOI":"10.3390\/cells9112367","volume":"9","author":"N Wagner","year":"2020","unstructured":"Wagner N, Wagner KD. The role of PPARs in disease. Cells. 2020;9:2367.","journal-title":"Cells"},{"key":"2346_CR48","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1016\/j.intimp.2019.04.012","volume":"72","author":"Y Liu","year":"2019","unstructured":"Liu Y, Qu Y, Liu L, Zhao H, Ma H, Si M, Cheng L, Nie L. PPAR-\u03b3 agonist pioglitazone protects against IL-17 induced intervertebral disc inflammation and degeneration via suppression of NF-\u03baB signaling pathway. Int Immunopharmacol. 2019;72:138\u201347.","journal-title":"Int Immunopharmacol"},{"key":"2346_CR49","doi-asserted-by":"publisher","first-page":"2541","DOI":"10.1002\/tox.23368","volume":"36","author":"G Zhang","year":"2021","unstructured":"Zhang G, Wang H, Zhang Q, Zhao Z, Zhu W, Zuo X. Bergenin alleviates H(2) O(2) -induced oxidative stress and apoptosis in nucleus pulposus cells: involvement of the PPAR-\u03b3\/NF-\u03baB pathway. Environ Toxicol. 2021;36:2541\u201350.","journal-title":"Environ Toxicol"},{"key":"2346_CR50","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1111\/j.1399-0004.2004.00250.x","volume":"65","author":"J Wang","year":"2004","unstructured":"Wang J, Huff AM, Spence JD, Hegele RA. Single nucleotide polymorphism in CTH associated with variation in plasma homocysteine concentration. Clin Genet. 2004;65:483\u20136.","journal-title":"Clin Genet"},{"key":"2346_CR51","doi-asserted-by":"publisher","first-page":"1355","DOI":"10.2741\/4286","volume":"19","author":"K Zhao","year":"2014","unstructured":"Zhao K, Li H, Li S, Yang G. Regulation of cystathionine gamma-lyase\/H\u2082S system and its pathological implication. Front Biosci (Landmark edition). 2014;19:1355\u201369.","journal-title":"Front Biosci (Landmark edition)"},{"key":"2346_CR52","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2023.110289","volume":"120","author":"K Wei","year":"2023","unstructured":"Wei K, Shu Z, Pu H, Xu H, Li S, Xiao J, Zhu Y, Ma T. Cystathionine-\u03b3-lyase attenuates inflammatory response and pain of osteoarthritis. Int Immunopharmacol. 2023;120: 110289.","journal-title":"Int Immunopharmacol"},{"key":"2346_CR53","first-page":"4026","volume":"12","author":"LC Mattheakis","year":"1992","unstructured":"Mattheakis LC, Shen WH, Collier RJ. DPH5, a methyltransferase gene required for diphthamide biosynthesis in Saccharomyces cerevisiae. Mol Cell Biol. 1992;12:4026\u201337.","journal-title":"Mol Cell Biol"},{"key":"2346_CR54","doi-asserted-by":"publisher","first-page":"479","DOI":"10.3390\/cells8050479","volume":"8","author":"B Ren","year":"2019","unstructured":"Ren B, Huang Y, Zou C, Wu Y, Huang Y, Ni J, Tian J. Transcriptional regulation of selenoprotein F by heat shock factor 1 during selenium supplementation and stress response. Cells. 2019;8:479.","journal-title":"Cells"},{"key":"2346_CR55","first-page":"119","volume":"22","author":"HJ Wu","year":"2003","unstructured":"Wu HJ, Lin C, Zha YY, Yang JG, Zhang MC, Zhang XY, Liang X, Fu M, Wu M. Redox reactions of Sep15 and its relationship with tumor development. Ai zheng Aizheng Chin J Cancer. 2003;22:119\u201322.","journal-title":"Ai zheng Aizheng Chin J Cancer"},{"key":"2346_CR56","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1080\/09674845.2017.1333241","volume":"75","author":"S Mohammaddoust","year":"2018","unstructured":"Mohammaddoust S, Salehi Z, Saeidi SH. SEPP1 and SEP15 gene polymorphisms and susceptibility to breast cancer. Br J Biomed Sci. 2018;75:36\u20139.","journal-title":"Br J Biomed Sci"}],"container-title":["European Journal of Medical Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s40001-025-02346-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s40001-025-02346-4\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s40001-025-02346-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,14]],"date-time":"2025-02-14T18:33:42Z","timestamp":1739558022000},"score":1,"resource":{"primary":{"URL":"https:\/\/eurjmedres.biomedcentral.com\/articles\/10.1186\/s40001-025-02346-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,14]]},"references-count":56,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["2346"],"URL":"https:\/\/doi.org\/10.1186\/s40001-025-02346-4","relation":{},"ISSN":["2047-783X"],"issn-type":[{"value":"2047-783X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,14]]},"assertion":[{"value":"15 November 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 January 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 February 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Ethical approval was obtained from the Soochow University (number: SUDA20231027A03). All methods and experimental protocols were carried out in accordance with the guidelines and regulations of the Care and Use of Laboratory Animals of Soochow University and the principles on ethical animal research outlined in the Basel Declaration and the ethical guidelines by the International Council for Laboratory Animal Science (ICLAS).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"102"}}