{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T16:25:21Z","timestamp":1791908721590,"version":"4.3.4"},"reference-count":39,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T00:00:00Z","timestamp":1763424000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T00:00:00Z","timestamp":1763424000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"name":"the Specific Research Fund of The Innovation Platform for Academicians of Hainan Province","award":["YSPTZX202311"],"award-info":[{"award-number":["YSPTZX202311"]}]},{"name":"the Key R&D Projects of Hainan Province","award":["ZDYF2022SHFZ074, ZDYF2022SHFZ280, ZDYF2017086, and ZDYF2019157"],"award-info":[{"award-number":["ZDYF2022SHFZ074, ZDYF2022SHFZ280, ZDYF2017086, and ZDYF2019157"]}]},{"DOI":"10.13039\/501100004761","name":"the Natural Science Foundation of Hainan Province","doi-asserted-by":"crossref","award":["820RC771"],"award-info":[{"award-number":["820RC771"]}],"id":[{"id":"10.13039\/501100004761","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82160544 and 82260304"],"award-info":[{"award-number":["82160544 and 82260304"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Eur J Med Res"],"DOI":"10.1186\/s40001-025-03402-9","type":"journal-article","created":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T11:58:24Z","timestamp":1763467104000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Effect of sperm DNA fragmentation on embryo euploidy rate in assisted reproductive technologies: a systematic review and meta-analysis"],"prefix":"10.1186","volume":"30","author":[{"given":"Bangbei","family":"Wan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiying","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,11,18]]},"reference":[{"issue":"8","key":"3402_CR1","doi-asserted-by":"publisher","first-page":"1746","DOI":"10.1093\/humrep\/deac131","volume":"37","author":"SM Diakiw","year":"2022","unstructured":"Diakiw SM, Hall JMM, VerMilyea MD, Amin J, Aizpurua J, Giardini L, et al. Development of an artificial intelligence model for predicting the likelihood of human embryo euploidy based on blastocyst images from multiple imaging systems during IVF. Hum Reprod. 2022;37(8):1746\u201359.","journal-title":"Hum Reprod"},{"key":"3402_CR2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21124381","author":"E Greco","year":"2020","unstructured":"Greco E, Litwicka K, Minasi MG, Cursio E, Greco PF, Barillari P. Preimplantation genetic testing: where we are today. Int J Mol Sci. 2020. https:\/\/doi.org\/10.3390\/ijms21124381.","journal-title":"Int J Mol Sci"},{"key":"3402_CR3","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2023.1178294","volume":"14","author":"Z Liang","year":"2023","unstructured":"Liang Z, Wen Q, Li J, Zeng D, Huang P. A systematic review and meta-analysis: clinical outcomes of recurrent pregnancy failure resulting from preimplantation genetic testing for aneuploidy. Front Endocrinol. 2023;14:1178294.","journal-title":"Front Endocrinol"},{"issue":"10","key":"3402_CR4","doi-asserted-by":"publisher","first-page":"2297","DOI":"10.1007\/s10815-023-02866-0","volume":"40","author":"LS Kasaven","year":"2023","unstructured":"Kasaven LS, Marcus D, Theodorou E, Jones BP, Saso S, Naja R, et al. Systematic review and meta-analysis: does pre-implantation genetic testing for aneuploidy at the blastocyst stage improve live birth rate? J Assist Reprod Genet. 2023;40(10):2297\u2013316.","journal-title":"J Assist Reprod Genet"},{"key":"3402_CR5","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-025-03575-6","author":"EAD Molin","year":"2025","unstructured":"Molin EAD, Pires GN, Lazzari VM. Live birth and other reproductive outcomes of mosaic and euploid embryos: a systematic review and meta-analysis. J Assist Reprod Genet. 2025. https:\/\/doi.org\/10.1007\/s10815-025-03575-6.","journal-title":"J Assist Reprod Genet"},{"issue":"5","key":"3402_CR6","doi-asserted-by":"publisher","first-page":"1787","DOI":"10.1007\/s00404-024-07370-z","volume":"309","author":"Y Tian","year":"2024","unstructured":"Tian Y, Li M, Yang J, Chen H, Lu D. Preimplantation genetic testing in the current era, a review. Arch Gynecol Obstet. 2024;309(5):1787\u201399.","journal-title":"Arch Gynecol Obstet"},{"key":"3402_CR7","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrrev.2020.108320","volume":"785","author":"M Mikwar","year":"2020","unstructured":"Mikwar M, MacFarlane AJ, Marchetti F. Mechanisms of oocyte aneuploidy associated with advanced maternal age. Mutat Res Rev Mutat Res. 2020;785:108320.","journal-title":"Mutat Res Rev Mutat Res"},{"issue":"8","key":"3402_CR8","doi-asserted-by":"publisher","first-page":"1605","DOI":"10.1111\/andr.13362","volume":"11","author":"OM Kocur","year":"2023","unstructured":"Kocur OM, Xie P, Cheung S, Souness S, McKnight M, Rosenwaks Z, et al. Can a sperm selection technique improve embryo ploidy? Andrology. 2023;11(8):1605\u201312.","journal-title":"Andrology"},{"issue":"5","key":"3402_CR9","doi-asserted-by":"publisher","first-page":"603","DOI":"10.1016\/j.eururo.2021.08.014","volume":"80","author":"S Minhas","year":"2021","unstructured":"Minhas S, Bettocchi C, Boeri L, Capogrosso P, Carvalho J, Cilesiz NC, et al. European association of urology guidelines on male sexual and reproductive health: 2021 update on male infertility. Eur Urol. 2021;80(5):603\u201320.","journal-title":"Eur Urol"},{"issue":"2","key":"3402_CR10","first-page":"306","volume":"28","author":"SW Andrabi","year":"2024","unstructured":"Andrabi SW, Ara A, Saharan A, Jaffar M, Gugnani N, Esteves SC. Sperm DNA fragmentation: causes, evaluation and management in male infertility. JBRA Assist Reprod. 2024;28(2):306\u201319.","journal-title":"JBRA Assist Reprod"},{"issue":"1","key":"3402_CR11","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1186\/s12958-023-01054-0","volume":"21","author":"A Szabo","year":"2023","unstructured":"Szabo A, Vancsa S, Hegyi P, Varadi A, Forintos A, Filipov T, et al. Lifestyle-, environmental-, and additional health factors associated with an increased sperm DNA fragmentation: a systematic review and meta-analysis. Reprod Biol Endocrinol. 2023;21(1):5.","journal-title":"Reprod Biol Endocrinol"},{"issue":"5","key":"3402_CR12","doi-asserted-by":"publisher","first-page":"1175","DOI":"10.1093\/humrep\/deab004","volume":"36","author":"RJ Aitken","year":"2021","unstructured":"Aitken RJ, Bakos HW. Should we be measuring DNA damage in human spermatozoa? New light on an old question. Hum Reprod. 2021;36(5):1175\u201385.","journal-title":"Hum Reprod"},{"issue":"1","key":"3402_CR13","doi-asserted-by":"publisher","first-page":"408","DOI":"10.1007\/s00345-024-05090-2","volume":"42","author":"I Ziouziou","year":"2024","unstructured":"Ziouziou I, Rambhatla A, Shah R, Agarwal A. Sperm DNA fragmentation and infertility: a narrative review. World J Urol. 2024;42(1):408.","journal-title":"World J Urol"},{"issue":"3","key":"3402_CR14","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1016\/j.fertnstert.2019.04.029","volume":"112","author":"E Borges Jr.","year":"2019","unstructured":"Borges E Jr., Zanetti BF, Setti AS, Braga D, Provenza RR, Iaconelli A Jr. Sperm DNA fragmentation is correlated with poor embryo development, lower implantation rate, and higher miscarriage rate in reproductive cycles of non-male factor infertility. Fertil Steril. 2019;112(3):483\u201390.","journal-title":"Fertil Steril"},{"issue":"2","key":"3402_CR15","doi-asserted-by":"publisher","first-page":"178","DOI":"10.23736\/S0031-0808.18.03534-6","volume":"61","author":"LS Lopes","year":"2019","unstructured":"Lopes LS, Esteves SC. Testicular sperm for intracytoplasmic sperm injection in non-azoospermic men: a paradigm shift. Panminerva Med. 2019;61(2):178\u201386.","journal-title":"Panminerva Med"},{"issue":"2","key":"3402_CR16","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1530\/REP-23-0011","volume":"166","author":"J Gao","year":"2023","unstructured":"Gao J, Yan Z, Yan L, Zhu X, Jiang H, Qiao J. The effect of sperm DNA fragmentation on the incidence and origin of whole and segmental chromosomal aneuploidies in human embryos. Reproduction. 2023;166(2):117\u201324.","journal-title":"Reproduction"},{"issue":"5","key":"3402_CR17","doi-asserted-by":"publisher","first-page":"872","DOI":"10.1080\/14647273.2021.1920054","volume":"25","author":"F Asgari","year":"2022","unstructured":"Asgari F, Gavahi A, Karimi M, Vatannejad A, Amjadi F, Aflatoonian R, et al. Risk of embryo aneuploidy is affected by the increase in sperm DNA damage in recurrent implantation failure patients under ICSI-CGH array cycles. Hum Fertil. 2022;25(5):872\u201380.","journal-title":"Hum Fertil"},{"key":"3402_CR18","doi-asserted-by":"publisher","first-page":"21","DOI":"10.4137\/CMRH.S32769","volume":"9","author":"J Garcia-Ferreyra","year":"2015","unstructured":"Garcia-Ferreyra J, Luna D, Villegas L, Romero R, Zavala P, Hilario R, et al. High aneuploidy rates observed in embryos derived from donated oocytes are related to male aging and high percentages of sperm DNA fragmentation. Clin Med Insights Reprod Health. 2015;9:21\u20137.","journal-title":"Clin Med Insights Reprod Health"},{"issue":"3","key":"3402_CR19","doi-asserted-by":"publisher","first-page":"997","DOI":"10.1007\/s00404-023-07098-2","volume":"308","author":"P Ping","year":"2023","unstructured":"Ping P, Liu Y, Zheng Z, Ma Y, Dong F, Chen X. Association of embryo aneuploidy and sperm DNA damage in unexplained recurrent implantation failure patients under NGS-based PGT-A cycles. Arch Gynecol Obstet. 2023;308(3):997\u20131005.","journal-title":"Arch Gynecol Obstet"},{"key":"3402_CR20","doi-asserted-by":"publisher","DOI":"10.1111\/andr.70079","author":"P Kong","year":"2025","unstructured":"Kong P, Liang S, Pan J, Yin M, Teng X. Sperm SCSA DNA fragmentation index does not influence the euploidy rate of viable blastocysts in advanced-age women. Andrology. 2025. https:\/\/doi.org\/10.1111\/andr.70079.","journal-title":"Andrology"},{"issue":"2","key":"3402_CR21","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1016\/j.xfre.2023.04.006","volume":"4","author":"AL Broussard","year":"2023","unstructured":"Broussard AL, Leader B, Tirado E, Russell H, Beydoun H, Colver R, et al. Sperm deoxyribonucleic acid fragmentation index at the time of intracytoplasmic sperm injection and standard in vitro fertilization is correlated with lower fertilization but not with blastocyst genetic diagnosis. F&S Reports. 2023;4(2):183\u20139.","journal-title":"F&S Reports"},{"issue":"1","key":"3402_CR22","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1007\/s10815-019-01632-5","volume":"37","author":"KA Green","year":"2020","unstructured":"Green KA, Patounakis G, Dougherty MP, Werner MD, Scott RT Jr, Franasiak JM. Sperm DNA fragmentation on the day of fertilization is not associated with embryologic or clinical outcomes after IVF\/ICSI. J Assist Reprod Genet. 2020;37(1):71\u20136.","journal-title":"J Assist Reprod Genet"},{"issue":"6","key":"3402_CR23","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2023.103366","volume":"47","author":"W Fu","year":"2023","unstructured":"Fu W, Cui Q, Yang Z, Bu Z, Shi H, Bi B, et al. High sperm DNA fragmentation increased embryo aneuploidy rate in patients undergoing preimplantation genetic testing. Reprod Biomed Online. 2023;47(6):103366.","journal-title":"Reprod Biomed Online"},{"key":"3402_CR24","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2022.1072176","volume":"13","author":"H Yang","year":"2022","unstructured":"Yang H, Liu Y, Niu W, Yang Z, Wang Y, Jin H, et al. Correlation study of male semen parameters and embryo aneuploidy in preimplantation genetic testing for aneuploidy. Front Endocrinol. 2022;13:1072176.","journal-title":"Front Endocrinol"},{"issue":"6","key":"3402_CR25","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0179002","volume":"12","author":"I Gat","year":"2017","unstructured":"Gat I, Tang K, Quach K, Kuznyetsov V, Antes R, Filice M, et al. Sperm DNA fragmentation index does not correlate with blastocyst aneuploidy or morphological grading. PLoS ONE. 2017;12(6):e0179002.","journal-title":"PLoS ONE"},{"issue":"8","key":"3402_CR26","doi-asserted-by":"publisher","first-page":"1682","DOI":"10.1111\/andr.13435","volume":"11","author":"D Braga","year":"2023","unstructured":"Braga D, Setti A, Morishima C, Provenza RR, Iaconelli A Jr., Borges E Jr. The effect of sperm DNA fragmentation on ICSI outcomes depending on oocyte quality. Andrology. 2023;11(8):1682\u201393.","journal-title":"Andrology"},{"issue":"16","key":"3402_CR27","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aaz7602","volume":"6","author":"S Middelkamp","year":"2020","unstructured":"Middelkamp S, van Tol HTA, Spierings DCJ, Boymans S, Guryev V, Roelen BAJ, et al. Sperm DNA damage causes genomic instability in early embryonic development. Sci Adv. 2020;6(16):eaaz7602.","journal-title":"Sci Adv"},{"issue":"5","key":"3402_CR28","doi-asserted-by":"publisher","first-page":"616","DOI":"10.1016\/S1472-6483(10)61188-1","volume":"12","author":"YJ Menezo","year":"2006","unstructured":"Menezo YJ. Paternal and maternal factors in preimplantation embryogenesis: interaction with the biochemical environment. Reprod Biomed Online. 2006;12(5):616\u201321.","journal-title":"Reprod Biomed Online"},{"issue":"6","key":"3402_CR29","doi-asserted-by":"publisher","DOI":"10.1016\/j.beem.2020.101477","volume":"34","author":"L Rodrigo","year":"2020","unstructured":"Rodrigo L. Sperm genetic abnormalities and their contribution to embryo aneuploidy & miscarriage. Best Pract Res Clin Endocrinol Metab. 2020;34(6):101477.","journal-title":"Best Pract Res Clin Endocrinol Metab"},{"issue":"6","key":"3402_CR30","doi-asserted-by":"publisher","first-page":"955","DOI":"10.1016\/j.rbmo.2019.09.013","volume":"39","author":"X Liang","year":"2019","unstructured":"Liang X, Mao Y, Wang Y, Liu S, Yan J. Female age affects the utility of sperm DNA fragmentation in predicting IVF and ICSI outcomes. Reprod Biomed Online. 2019;39(6):955\u201362.","journal-title":"Reprod Biomed Online"},{"key":"3402_CR31","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.918746","volume":"26","author":"Y Qiu","year":"2020","unstructured":"Qiu Y, Yang H, Li C, Xu C. Progress in research on sperm DNA fragmentation. Med Sci Monit. 2020;26:e918746.","journal-title":"Med Sci Monit"},{"issue":"1","key":"3402_CR32","doi-asserted-by":"publisher","first-page":"F29","DOI":"10.1530\/REP-17-0683","volume":"156","author":"DK Griffin","year":"2018","unstructured":"Griffin DK, Ogur C. Chromosomal analysis in IVF: just how useful is it? Reproduction. 2018;156(1):F29\u201350.","journal-title":"Reproduction"},{"key":"3402_CR33","doi-asserted-by":"publisher","DOI":"10.3390\/ijms252011296","author":"E Moustakli","year":"2024","unstructured":"Moustakli E, Gkountis A, Dafopoulos S, Zikopoulos A, Sotiriou S, Zachariou A, et al. Comparative analysis of fluorescence in situ hybridization and next-generation sequencing in sperm evaluation: implications for preimplantation genetic testing and male infertility. Int J Mol Sci. 2024. https:\/\/doi.org\/10.3390\/ijms252011296.","journal-title":"Int J Mol Sci"},{"issue":"1","key":"3402_CR34","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1007\/s10815-018-1347-6","volume":"36","author":"KL Palmerola","year":"2019","unstructured":"Palmerola KL, Vitez SF, Amrane S, Fischer CP, Forman EJ. Minimizing mosaicism: assessing the impact of fertilization method on rate of mosaicism after next-generation sequencing (NGS) preimplantation genetic testing for aneuploidy (PGT-A). J Assist Reprod Genet. 2019;36(1):153\u20137.","journal-title":"J Assist Reprod Genet"},{"issue":"1","key":"3402_CR35","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1007\/s43032-023-01301-0","volume":"31","author":"C Morales","year":"2024","unstructured":"Morales C. Current applications and controversies in preimplantation genetic testing for aneuploidies (PGT-A) in in vitro fertilization. Reprod Sci. 2024;31(1):66\u201380.","journal-title":"Reprod Sci"},{"issue":"1","key":"3402_CR36","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1016\/j.fertnstert.2019.03.003","volume":"112","author":"DB McQueen","year":"2019","unstructured":"McQueen DB, Zhang J, Robins JC. Sperm DNA fragmentation and recurrent pregnancy loss: a systematic review and meta-analysis. Fertil Steril. 2019;112(1):54\u201360.","journal-title":"Fertil Steril"},{"issue":"4","key":"3402_CR37","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1016\/j.rbmo.2013.06.014","volume":"27","author":"SE Lewis","year":"2013","unstructured":"Lewis SE, John Aitken R, Conner SJ, Iuliis GD, Evenson DP, Henkel R, et al. The impact of sperm DNA damage in assisted conception and beyond: recent advances in diagnosis and treatment. Reprod Biomed Online. 2013;27(4):325\u201337.","journal-title":"Reprod Biomed Online"},{"issue":"3","key":"3402_CR38","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1097\/GCO.0000000000000271","volume":"28","author":"A Agarwal","year":"2016","unstructured":"Agarwal A, Cho CL, Esteves SC. Should we evaluate and treat sperm DNA fragmentation? Curr Opin Obstet Gynecol. 2016;28(3):164\u201371.","journal-title":"Curr Opin Obstet Gynecol"},{"key":"3402_CR39","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2023.1150951","volume":"14","author":"M Romano","year":"2023","unstructured":"Romano M, Cirillo F, Spadaro D, Busnelli A, Castellano S, Albani E, et al. High sperm DNA fragmentation: do we have robust evidence to support antioxidants and testicular sperm extraction to improve fertility outcomes? A narrative review. Front Endocrinol. 2023;14:1150951.","journal-title":"Front Endocrinol"}],"container-title":["European Journal of Medical Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s40001-025-03402-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s40001-025-03402-9\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s40001-025-03402-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T00:05:43Z","timestamp":1763510743000},"score":1,"resource":{"primary":{"URL":"https:\/\/eurjmedres.biomedcentral.com\/articles\/10.1186\/s40001-025-03402-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,18]]},"references-count":39,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["3402"],"URL":"https:\/\/doi.org\/10.1186\/s40001-025-03402-9","relation":{},"ISSN":["2047-783X"],"issn-type":[{"value":"2047-783X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,18]]},"assertion":[{"value":"19 August 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 October 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 November 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"1138"}}