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Growing evidence has supported that the same protein can induce remarkably distinct pathological progresses and disease phenotypes, suggesting the existence of strain difference among \u03b1-syn fibrils. Previous studies have shown that \u03b1-syn pathology can propagate from the peripheral nervous system (PNS) to the\u00a0central nervous system (CNS) in a \u201cprion-like\u201d manner. However, the difference of the propagation potency from the periphery to CNS among different \u03b1-syn strains remains unknown and the effect of different generation processes of these strains on the potency of seeding and propagation remains to be revealed in more detail.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Three strains of preformed \u03b1-syn fibrils (PFFs) were generated in different buffer conditions which varied in pH and ionic concentrations. The \u03b1-syn PFFs were intramuscularly (IM) injected into a novel bacterial artificial chromosome (BAC) transgenic mouse line that expresses wild-type human \u03b1-syn, and the efficiency of seeding and propagation of these PFFs from the PNS to the CNS was evaluated.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The three strains of \u03b1-syn PFFs triggered distinct propagation patterns. The fibrils generated in mildly acidic buffer led to the most severe \u03b1-syn pathology, degeneration of motor neurons and microgliosis in the spinal cord.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>The different \u03b1-syn conformers generated in different conditions exhibited strain-specific pathology and propagation patterns from the periphery to the CNS, which further supports the view that \u03b1-syn strains may be responsible for the heterogeneity of pathological features and disease progresses among synucleinopathies.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s40035-021-00242-5","type":"journal-article","created":{"date-parts":[[2021,6,21]],"date-time":"2021-06-21T03:32:21Z","timestamp":1624246341000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Differential seeding and propagating efficiency of \u03b1-synuclein strains generated in different conditions"],"prefix":"10.1186","volume":"10","author":[{"given":"Di","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian-Jun","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji-Hui","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Svanbergsson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Haikal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gunnar","family":"Gouras","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7770-7376","authenticated-orcid":false,"given":"Jia-Yi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,6,21]]},"reference":[{"key":"242_CR1","doi-asserted-by":"crossref","unstructured":"Spillantini MG, Goedert M. 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