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These systems use fluids in microchannels (1 to 300\u00a0microns) to analyze extremely small volumes of sample and reagent, allowing precise delivery and mixing while maintaining accurate results. Parkinson's disease (PD) poses significant diagnostic challenges, with early detection being critical to improved treatment outcomes. A key pathological feature of PD is the presence of Lewy bodies composed of \u03b1-synuclein (\u03b1Syn) fibrils. Recent research has shown that \u03b1Syn oligomers can be toxic and contribute to neuronal loss. Therefore, microfluidics offers a promising approach for the diagnosis of different stages of \u03b1Syn pathology. This review comprehensively analyzes the application of microfluidics in single-cell analysis and protein aggregation studies. We discuss the concept of lab-on-a-chip analysis and examine different substrates for \u03b1Syn detection, citing relevant studies and expected protein concentrations and their correlations with disease progression and severity.<\/jats:p>","DOI":"10.1186\/s40035-025-00526-0","type":"journal-article","created":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T02:53:42Z","timestamp":1766544822000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Microfluidic platform for understanding Parkinson\u2019s disease and \u03b1-synuclein conformation"],"prefix":"10.1186","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3650-6121","authenticated-orcid":false,"given":"Tregub","family":"Pavel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0825-8909","authenticated-orcid":false,"given":"Zembatov","family":"Georgy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3725-6360","authenticated-orcid":false,"given":"Namiot","family":"Eugenia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7853-6222","authenticated-orcid":false,"given":"Kolotyeva","family":"Natalia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6821-904X","authenticated-orcid":false,"given":"Yurchenko","family":"Stanislav","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2704-6282","authenticated-orcid":false,"given":"Illarioshkin","family":"Sergey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4012-6348","authenticated-orcid":false,"given":"Salmina","family":"Alla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,12,24]]},"reference":[{"key":"526_CR1","doi-asserted-by":"publisher","first-page":"939","DOI":"10.1016\/S1474-4422(18)30295-3","volume":"17","author":"ER Dorsey","year":"2018","unstructured":"Dorsey ER, Elbaz A, Nichols E, Abd-Allah F, Abdelalim A, Adsuar JC, et al. 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