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As a major Ca<jats:sup>2+<\/jats:sup> release channel of endoplasmic reticulum (ER), ryanodine receptor 1 (RyR1) key mutations are main causes of severe congenital myopathies. The role of RyR1 in myogenic differentiation has attracted intense research interest but remains unclear.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>In the present study, both RyR1-knockdown myoblasts and CRISPR\/Cas9-based RyR1-knockout myoblasts were employed to explore the role of RyR1 in myogenic differentiation, myotube formation as well as the potential mechanism of RyR1-related myopathies. We observed that RyR1 expression was dramatically increased during the late stage of myogenic differentiation, accompanied by significantly elevated cytoplasmic Ca<jats:sup>2+<\/jats:sup> concentration. Inhibition of RyR1 by siRNA-mediated knockdown or chemical inhibitor, dantrolene, significantly reduced cytosolic Ca<jats:sup>2+<\/jats:sup> and blocked multinucleated myotube formation. The elevation of cytoplasmic Ca<jats:sup>2+<\/jats:sup> concentration can effectively relieve myogenic differentiation stagnation by RyR1 inhibition, demonstrating that RyR1 modulates myogenic differentiation via regulation of Ca<jats:sup>2+<\/jats:sup> release channel. However, RyR1-knockout-induced Ca<jats:sup>2+<\/jats:sup> leakage led to the severe ER stress and excessive unfolded protein response, and drove myoblasts into apoptosis.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Therefore, we concluded that Ca<jats:sup>2+<\/jats:sup> release mediated by dramatic increase in RyR1 expression is required for the late stage of myogenic differentiation and fusion. This study contributes to a novel understanding of the role of RyR1 in myogenic differentiation and related congenital myopathies, and provides a potential target for regulation of muscle characteristics and meat quality.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s40104-021-00668-x","type":"journal-article","created":{"date-parts":[[2022,2,11]],"date-time":"2022-02-11T00:02:56Z","timestamp":1644537776000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Ryanodine receptor RyR1-mediated elevation of Ca2+ concentration is required for the late stage of myogenic differentiation and fusion"],"prefix":"10.1186","volume":"13","author":[{"given":"Kai","family":"Qiu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yubo","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doudou","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linjuan","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enfa","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4623-0568","authenticated-orcid":false,"given":"Jingdong","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,2,11]]},"reference":[{"issue":"6258","key":"668_CR1","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1038\/343559a0","volume":"343","author":"DH Maclennan","year":"1990","unstructured":"Maclennan DH, Duff C, Zorzato F, Fujii J, Phillips M, Korneluk RG, et al. 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