{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T05:28:12Z","timestamp":1792387692843,"version":"4.4.0"},"reference-count":39,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T00:00:00Z","timestamp":1715126400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T00:00:00Z","timestamp":1715126400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Animal Sci Biotechnol"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Thermal stress in subtropical regions is a major limiting factor in beef cattle production systems with around $369 million being lost annually due to reduced performance. Heat stress causes numerous physiological and behavioral disturbances including reduced feed intake and decreased production levels. Cattle utilize various physiological mechanisms such as sweating to regulate internal heat. Variation in these traits can help identify genetic variants that control sweat gland properties and subsequently allow for genetic selection of cattle with greater thermotolerance.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>This study used 2,401 Brangus cattle from two commercial ranches in Florida. Precise phenotypes that contribute to an animal\u2019s ability to manage heat stress were calculated from skin biopsies and included sweat gland area, sweat gland depth, and sweat gland length. All animals were genotyped with the Bovine GGP F250K, and BLUPF90 software was used to estimate genetic parameters and for Genome Wide Association Study.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Sweat gland phenotypes heritability ranged from 0.17 to 0.42 indicating a moderate amount of the phenotypic variation is due to genetics, allowing producers the ability to select for favorable sweat gland properties. A weighted single-step GWAS using sliding 10\u2009kb windows identified multiple quantitative trait loci (QTLs) explaining a significant amount of genetic variation. QTLs located on BTA7 and BTA12 explained over 1.0% of genetic variance and overlap the <jats:italic>ADGRV1<\/jats:italic> and <jats:italic>CCDC168<\/jats:italic> genes<jats:italic>,<\/jats:italic> respectively. The variants identified in this study are implicated in processes related to immune function and cellular proliferation which could be relevant to heat management. Breed of Origin Alleles (BOA) were predicted using local ancestry in admixed populations (LAMP-LD), allowing for identification of markers\u2019 origin from either Brahman or Angus ancestry. A BOA GWAS was performed to identify regions inherited from particular ancestral breeds that might have a significant impact on sweat gland phenotypes.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>The results of the BOA GWAS indicate that both Brahman and Angus alleles contribute positively to sweat gland traits, as evidenced by favorable marker effects observed from both genetic backgrounds. Understanding and utilizing genetic traits that confer better heat tolerance is a proactive approach to managing the impacts of climate change on livestock farming.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s40104-024-01025-4","type":"journal-article","created":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T00:01:44Z","timestamp":1715126504000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Exploring the genetic control of sweat gland characteristics in beef cattle for enhanced heat tolerance"],"prefix":"10.1186","volume":"15","author":[{"given":"Aakilah S.","family":"Hernandez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel A.","family":"Zayas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo E.","family":"Rodriguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaitlyn M.","family":"Sarlo Davila","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fahad","family":"Rafiq","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea N.","family":"Nunez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristiane Gon\u00e7alves","family":"Titto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2661-1198","authenticated-orcid":false,"given":"Raluca G.","family":"Mateescu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,5,8]]},"reference":[{"issue":"2","key":"1025_CR1","doi-asserted-by":"publisher","first-page":"skaa014","DOI":"10.1093\/jas\/skaa014","volume":"98","author":"RF Cooke","year":"2020","unstructured":"Cooke RF, Daigle CL, Moriel P, Smith SB, Tedeschi LO, Vendramini JMB. Cattle adapted to tropical and subtropical environments: social, nutritional, and carcass quality considerations. J Anim Sci. 2020;98(2):skaa014.","journal-title":"J Anim Sci."},{"issue":"6","key":"1025_CR2","doi-asserted-by":"publisher","first-page":"322","DOI":"10.3390\/ani9060322","volume":"9","author":"AM Lees","year":"2019","unstructured":"Lees AM, Sejian V, Wallage AL, Steel CC, Mader TL, Lees JC, et al. The impact of heat load on cattle. Animals (Basel). 2019;9(6):322.","journal-title":"Animals (Basel)."},{"key":"1025_CR3","doi-asserted-by":"publisher","first-page":"E52","DOI":"10.3168\/jds.S0022-0302(03)74040-5","volume":"86","author":"NR St-Pierre","year":"2003","unstructured":"St-Pierre NR, Cobanov B, Schnitkey G. Economic losses from heat stress by US livestock industries. J Dairy Sci. 2003;86:E52\u2013774.","journal-title":"J Dairy Sci."},{"key":"1025_CR4","doi-asserted-by":"publisher","first-page":"513","DOI":"10.1146\/annurev-animal-022114-110659","volume":"3","author":"RJ Collier","year":"2015","unstructured":"Collier RJ, Gebremedhin KG. Thermal biology of domestic animals. Ann Rev Anim Biosci. 2015;3:513\u201332.","journal-title":"Ann Rev Anim Biosci."},{"issue":"5","key":"1025_CR5","doi-asserted-by":"publisher","first-page":"1224","DOI":"10.2527\/jas1980.5151224x","volume":"51","author":"KE Gregory","year":"1980","unstructured":"Gregory KE, Cundiff LV. Crossbreeding in beef cattle: evaluation of systems. J Anim Sci. 1980;51(5):1224\u201342.","journal-title":"J Anim Sci."},{"issue":"2","key":"1025_CR6","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1017\/S1751731117001380","volume":"12","author":"DE Goszczynski","year":"2018","unstructured":"Goszczynski DE, Corbi-Botto CM, Durand HM, Rogberg-Mu\u00f1oz A, Munilla S, Peral-Garcia P, et al. Evidence of positive selection towards Zebuine haplotypes in the BoLA region of Brangus cattle. Animal. 2018;12(2):215\u201323.","journal-title":"Animal."},{"issue":"2","key":"1025_CR7","doi-asserted-by":"publisher","first-page":"531","DOI":"10.2527\/jas1986.622531x","volume":"62","author":"VA Finch","year":"1986","unstructured":"Finch VA. Body temperature in beef cattle: its control and relevance to production in the tropics. J Anim Sci. 1986;62(2):531\u201342.","journal-title":"J Anim Sci."},{"key":"1025_CR8","doi-asserted-by":"publisher","first-page":"1107468","DOI":"10.3389\/fgene.2023.1107468","volume":"14","author":"RG Mateescu","year":"2023","unstructured":"Mateescu RG, Sarlo Davila KM, Hernandez AS, Andrade AN, Zayas GA, Rodriguez EE, et al. Impact of Brahman genetics on skin histology characteristics with implications for heat tolerance in cattle. Front Genet. 2023;14:1107468.","journal-title":"Front Genet."},{"issue":"7","key":"1025_CR9","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/nmeth.2019","volume":"9","author":"J Schindelin","year":"2012","unstructured":"Schindelin J, Arganda-Carreras I, Frise E, Kaynig V, Longair M, Pietzsch T, et al. Fiji: an open-source platform for biological-image analysis. Nat Methods. 2012;9(7):676\u201382.","journal-title":"Nat Methods."},{"key":"1025_CR10","unstructured":"Misztal I, Lourenco D, Aguilar I, Legarra A, Vitezica Z. Manual for BLUPF90 family of programs. Athens: University of Georgia; 2014. http:\/\/nce.ads.uga.edu\/wiki\/doku.php."},{"issue":"11","key":"1025_CR11","doi-asserted-by":"publisher","first-page":"4414","DOI":"10.3168\/jds.2007-0980","volume":"91","author":"PM VanRaden","year":"2008","unstructured":"VanRaden PM. Efficient methods to compute genomic predictions. J Dairy Sci. 2008;91(11):4414\u201323.","journal-title":"J Dairy Sci."},{"issue":"2","key":"1025_CR12","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1017\/S0016672312000274","volume":"94","author":"H Wang","year":"2012","unstructured":"Wang H, Misztal I, Aguilar I, Legarra A, Muir WM. Genome-wide association mapping including phenotypes from relatives without genotypes. Genet Res. 2012;94(2):73\u201383.","journal-title":"Genet Res."},{"key":"1025_CR13","volume-title":"R: a language and environment for statistical computing [internet]","author":"R Core Team","year":"2021","unstructured":"R Core Team. R: a language and environment for statistical computing [internet]. Vienna, Austria: R Foundation for Statistical Computing; 2021."},{"issue":"D1","key":"1025_CR14","doi-asserted-by":"publisher","first-page":"D988","DOI":"10.1093\/nar\/gkab1049","volume":"50","author":"F Cunningham","year":"2022","unstructured":"Cunningham F, Allen JE, Allen J, Alvarez-Jarreta J, Amode MR, Armean IM, et al. Ensembl 2022. Nucleic Acids Res. 2022;50(D1):D988\u201395.","journal-title":"Nucleic Acids Res."},{"issue":"4","key":"1025_CR15","doi-asserted-by":"publisher","first-page":"R42","DOI":"10.1186\/gb-2009-10-4-r42","volume":"10","author":"AV Zimin","year":"2009","unstructured":"Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, et al. A whole-genome assembly of the domestic cow, Bos taurus. Genome Biol. 2009;10(4):R42.","journal-title":"Genome Biol."},{"issue":"10","key":"1025_CR16","doi-asserted-by":"publisher","first-page":"1359","DOI":"10.1093\/bioinformatics\/bts144","volume":"28","author":"Y Baran","year":"2012","unstructured":"Baran Y, Pasaniuc B, Sankararaman S, Torgerson DG, Gignoux C, Eng C, et al. Fast and accurate inference of local ancestry in Latino populations. Bioinformat. 2012;28(10):1359\u201367.","journal-title":"Bioinformat."},{"issue":"2","key":"1025_CR17","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1159\/000145118","volume":"105","author":"SF Amakiri","year":"1979","unstructured":"Amakiri SF, Adepoju JJ. Changes in sweat glands morphology in cattle before and during heat stimulation. Acta Anat (Basel). 1979;105(2):140\u201350.","journal-title":"Acta Anat (Basel)."},{"key":"1025_CR18","doi-asserted-by":"crossref","unstructured":"Rashamol VP, Sejian V, Bagath M, Krishnan G, Archana PR, Bhatta R. Physiological adaptability of livestock to heat stress: an updated review. J Anim Behav Biometeorol. 2018; 6:62\u201371.","DOI":"10.31893\/2318-1265jabb.v6n3p62-71"},{"issue":"4","key":"1025_CR19","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1071\/BI9750417","volume":"28","author":"DM Jenkinson","year":"1975","unstructured":"Jenkinson DM, Mason IL, Nay T. Inheritance of some sweat gland and hair follicle characteristics in cattle. Aust J Biol Sci. 1975;28(4):417\u201324.","journal-title":"Aust J Biol Sci."},{"issue":"5","key":"1025_CR20","first-page":"1659","volume":"141","author":"PE Sugier","year":"2018","unstructured":"Sugier PE, Brossard M, Sarnowski C, Vaysse A, Morin A, Pain L, et al. A novel role for ciliary function in atopy: ADGRV1 and DNAH5 interactions. Aust J Biol Sci. 2018;141(5):1659\u20131667.e11.","journal-title":"Aust J Biol Sci."},{"issue":"10","key":"1025_CR21","doi-asserted-by":"publisher","first-page":"8218","DOI":"10.3168\/jds.2022-22087","volume":"105","author":"N S\u00f6lzer","year":"2022","unstructured":"S\u00f6lzer N, May K, Yin T, K\u00f6nig S. Genomic analyses of claw disorders in Holstein cows: genetic parameters, trait associations, and genome-wide associations considering interactions of SNP and heat stress. J Dairy Sci. 2022;105(10):8218\u201336.","journal-title":"J Dairy Sci."},{"issue":"2","key":"1025_CR22","doi-asserted-by":"publisher","first-page":"294","DOI":"10.1002\/path.4843","volume":"241","author":"HM Mitchison","year":"2017","unstructured":"Mitchison HM, Valente EM. Motile and non-motile cilia in human pathology: from function to phenotypes. J Pathol. 2017;241(2):294\u2013309.","journal-title":"J Pathol."},{"issue":"4","key":"1025_CR23","doi-asserted-by":"publisher","first-page":"e2002240","DOI":"10.1371\/journal.pbio.2002240","volume":"15","author":"P Sengupta","year":"2017","unstructured":"Sengupta P. Cilia and sensory signaling: the journey from \u201canimalcules\u201d to human disease. PLoS Biol. 2017;15(4):e2002240.","journal-title":"PLoS Biol."},{"issue":"4","key":"1025_CR24","doi-asserted-by":"publisher","first-page":"104167","DOI":"10.1016\/j.isci.2022.104167","volume":"25","author":"P Beck","year":"2022","unstructured":"Beck P, Selle B, Madenach L, Jones DTW, Vokuhl C, Gopisetty A, et al. The genomic landscape of pediatric renal cell carcinomas. iScience. 2022;25(4):104167.","journal-title":"iScience."},{"issue":"4","key":"1025_CR25","doi-asserted-by":"publisher","first-page":"636","DOI":"10.3390\/biom10040636","volume":"10","author":"J Kim","year":"2020","unstructured":"Kim J, Ryu C, Ha J, Lee J, Kim D, Ji M, et al. Structural and quantitative characterization of mucin-type o-glycans and the identification of o-glycosylation sites in bovine submaxillary mucin. Biomolecules. 2020;10(4):636.","journal-title":"Biomolecules."},{"issue":"11","key":"1025_CR26","doi-asserted-by":"publisher","first-page":"1471","DOI":"10.1016\/0300-9084(88)90284-2","volume":"70","author":"P Roussel","year":"1988","unstructured":"Roussel P, Lamblin G, Lhermitte M, Houdret N, Lafitte JJ, Perini JM, et al. The complexity of mucins. Biochimie. 1988;70(11):1471\u201382.","journal-title":"Biochimie."},{"issue":"8","key":"1025_CR27","doi-asserted-by":"publisher","first-page":"675","DOI":"10.1080\/14728222.2018.1498845","volume":"22","author":"A Aithal","year":"2018","unstructured":"Aithal A, Rauth S, Kshirsagar P, Shah A, Lakshmanan I, Junker WM, et al. MUC16 as a novel target for cancer therapy. Expert Opin Ther Targets. 2018;22(8):675\u201386.","journal-title":"Expert Opin Ther Targets."},{"issue":"16","key":"1025_CR28","doi-asserted-by":"publisher","first-page":"5047","DOI":"10.1021\/jp212495t","volume":"116","author":"Y Znamenskaya","year":"2012","unstructured":"Znamenskaya Y, Sotres J, Engblom J, Arnebrant T, Kocherbitov V. Effect of hydration on structural and thermodynamic properties of pig gastric and bovine submaxillary gland mucins. J Phys Chem B. 2012;116(16):5047\u201355.","journal-title":"J Phys Chem B."},{"issue":"1","key":"1025_CR29","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1095\/biolreprod.115.132951","volume":"94","author":"SG Moore","year":"2016","unstructured":"Moore SG, Pryce JE, Hayes BJ, Chamberlain AJ, Kemper KE, Berry DP, et al. Differentially expressed genes in endometrium and corpus luteum of Holstein cows selected for high and low fertility are enriched for sequence variants associated with fertility. Biol Reprod. 2016;94(1):1\u201311.","journal-title":"Biol Reprod."},{"issue":"16","key":"1025_CR30","doi-asserted-by":"publisher","first-page":"2052","DOI":"10.1080\/15384101.2018.1513764","volume":"17","author":"S Srivastava","year":"2018","unstructured":"Srivastava S, Panda D. A centrosomal protein STARD9 promotes microtubule stability and regulates spindle microtubule dynamics. Cell Cycle. 2018;17(16):2052\u201368. https:\/\/doi.org\/10.1080\/15384101.2018.1513764.","journal-title":"Cell Cycle"},{"issue":"9","key":"1025_CR31","doi-asserted-by":"publisher","first-page":"903","DOI":"10.1002\/bies.201600062","volume":"38","author":"L Truebestein","year":"2016","unstructured":"Truebestein L, Leonard TA. Coiled-coils: The long and short of it. Bioessays. 2016;38(9):903\u201316. https:\/\/doi.org\/10.1002\/bies.201600062.","journal-title":"Bioessays"},{"issue":"6","key":"1025_CR32","doi-asserted-by":"publisher","first-page":"965","DOI":"10.3390\/genes13060965","volume":"13","author":"Y Huang","year":"2022","unstructured":"Huang Y, Yuan C, Zhao Y, Li C, Cao M, Li H, et al. Identification and regulatory network analysis of genes related to reproductive performance in the hypothalamus and pituitary of Angus cattle. Genes (Basel). 2022;13(6):965.","journal-title":"Genes (Basel)."},{"issue":"3","key":"1025_CR33","doi-asserted-by":"publisher","first-page":"254","DOI":"10.1136\/jmedgenet-2021-108406","volume":"60","author":"R Zheng","year":"2023","unstructured":"Zheng R, Wang Y, Li Y, Guo J, Wen Y, Jiang C, et al. FSIP2 plays a role in the acrosome development during spermiogenesis. J Med Genet. 2023;60(3):254\u201364.","journal-title":"J Med Genet."},{"issue":"17","key":"1025_CR34","doi-asserted-by":"publisher","first-page":"2819","DOI":"10.7150\/ijms.48971","volume":"17","author":"Y Zhang","year":"2020","unstructured":"Zhang Y, Zhu X, Qiao X, Sun L, Tian Y, Yang Y, et al. FSIP2 can serve as a predictive biomarker for clear cell renal cell carcinoma prognosis. Int J Med Sci. 2020;17(17):2819\u201325.","journal-title":"Int J Med Sci."},{"issue":"1","key":"1025_CR35","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1530\/REP-19-0037","volume":"158","author":"RL Piersanti","year":"2019","unstructured":"Piersanti RL, Horlock AD, Block J, Santos JEP, Sheldon IM, Bromfield JJ. Persistent effects on bovine granulosa cell transcriptome after resolution of uterine disease. Reproduction. 2019;158(1):35\u201346. https:\/\/doi.org\/10.1530\/REP-19-0037.","journal-title":"Reproduction"},{"issue":"5","key":"1025_CR36","doi-asserted-by":"publisher","first-page":"482","DOI":"10.1071\/AR9560482","volume":"7","author":"T Nay","year":"1956","unstructured":"Nay T, Hayman RH, Nay T, Hayman RH. Sweat glands in zebu and European cattle. I. Size of individual glands, the denseness of their population, and their depth below the skin surface. Australian Jour Agric Res. 1956;7(5):482\u201394.","journal-title":"Australian Jour Agric Res."},{"key":"1025_CR37","doi-asserted-by":"publisher","first-page":"1569","DOI":"10.4102\/jsava.v89i0.1569","volume":"89","author":"D Moyo","year":"2018","unstructured":"Moyo D, Gomes M, Erlwanger KH. Comparison of the histology of the skin of the Windsnyer, Kolbroek and Large White pigs. J S Afr Vet Assoc. 2018;89:1569.","journal-title":"J S Afr Vet Assoc."},{"issue":"3","key":"1025_CR38","doi-asserted-by":"publisher","first-page":"254","DOI":"10.1136\/jmedgenet-2021-108406","volume":"60","author":"R Zheng","year":"2023","unstructured":"Zheng R, Wang Y, Li Y, Guo J, Wen Y, Jiang C, et al. FSIP2 plays a role in the acrosome development during spermiogenesis. J Med Genet. 2023;60(3):254\u201364. https:\/\/doi.org\/10.1136\/jmedgenet-2021-108406.","journal-title":"J Med Genet"},{"issue":"4","key":"1025_CR39","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1111\/bcpt.13869","volume":"133","author":"J Linnert","year":"2023","unstructured":"Linnert J, G\u00fcler BE, Krzysko J, Wolfrum U. The adhesion G protein-coupled receptor VLGR1\/ADGRV1 controls autophagy. Basic Clin Pharmacol Toxicol. 2023;133(4):313\u201330. https:\/\/doi.org\/10.1111\/bcpt.13869.","journal-title":"Basic Clin Pharmacol Toxicol"}],"container-title":["Journal of Animal Science and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s40104-024-01025-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s40104-024-01025-4\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s40104-024-01025-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T00:02:05Z","timestamp":1715126525000},"score":1,"resource":{"primary":{"URL":"https:\/\/jasbsci.biomedcentral.com\/articles\/10.1186\/s40104-024-01025-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,8]]},"references-count":39,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["1025"],"URL":"https:\/\/doi.org\/10.1186\/s40104-024-01025-4","relation":{},"ISSN":["2049-1891"],"issn-type":[{"value":"2049-1891","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,8]]},"assertion":[{"value":"26 December 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 March 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 May 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The project was approved by the Institutional Animal Care and Use Committee\u00a0of the University of Florida (201910547).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not Applicable","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"66"}}