{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T17:55:50Z","timestamp":1791568550863,"version":"4.3.4"},"reference-count":12,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,11,15]],"date-time":"2024-11-15T00:00:00Z","timestamp":1731628800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,11,15]],"date-time":"2024-11-15T00:00:00Z","timestamp":1731628800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"High-level Talent Innovation Support Program of Dalian Science and Technology Bureau","award":["2021RD02"],"award-info":[{"award-number":["2021RD02"]}]},{"name":"Liaoning Province International Science and Technology Cooperation Program","award":["2024JH2\/101900006"],"award-info":[{"award-number":["2024JH2\/101900006"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Exp Hematol Oncol"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The Patient-Derived Organoids (PDOs) has demonstrated significant potential in personalized medicine. However, the initial establishment of lung cancer organoids (LCOs), and timely therapeutic recommendations face several challenges. Particularly, the current culture systems have not yet achieved the capability to long-term cultivation of all lung tumor sample sources, including malignant pleural effusion (MPE), which poses significant barriers to the rapid clinical translation of PDOs. Here, we established a LCOs biobank derived from various tumor cell origins and investigated the impact of supplementing culture media with MPE supernatant on organoid formation, culture duration, and drug sensitivity. Our findings indicate that MPE can enhance the successful rate of LCOs by extending the passage number and promoting the initial formation of difficult-to-culture samples, such as those derived from MPE or cell lines that were previously unsuccessful in Airway Organoid (AO) medium. MPE also facilitates the rapid proliferation of LCOs, reducing the culture duration by over 50%. Additionally, LCOs exhibit increased chemoresistance in the presence of MPE, which modifies stem cell distribution and reshapes the internal structure of the organoids. Overall, this study highlights the significance of MPE in facilitating the establishment and maintenance of LCOs, and its potential for translational applications in lung cancer research and personalized.<\/jats:p>","DOI":"10.1186\/s40164-024-00581-9","type":"journal-article","created":{"date-parts":[[2024,11,15]],"date-time":"2024-11-15T08:00:27Z","timestamp":1731657627000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Malignant pleural effusion facilitates the establishment and maintenance of tumor organoid biobank with multiple patient-derived lung tumor cell sources"],"prefix":"10.1186","volume":"13","author":[{"given":"Lingwei","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanli","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanhua","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanjiao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiting","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyan","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruoyu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanshan","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,11,15]]},"reference":[{"issue":"1","key":"581_CR1","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1186\/s40164-023-00433-y","volume":"12","author":"Z Fang","year":"2023","unstructured":"Fang Z, Li P, Du F, et al. The role of organoids in cancer research. Experimental Hematol Oncol. 2023;12(1):69. https:\/\/doi.org\/10.1186\/s40164-023-00433-y","journal-title":"Experimental Hematol Oncol"},{"issue":"2","key":"581_CR2","doi-asserted-by":"publisher","first-page":"100911","DOI":"10.1016\/j.xcrm.2022.100911","volume":"4","author":"H Wang","year":"2023","unstructured":"Wang H, Zhang C, Peng K, et al. Using patient-derived organoids to predict locally advanced or metastatic lung cancer tumor response: a real-world study. Cell Rep Med. 2023;4(2):100911. https:\/\/doi.org\/10.1016\/j.xcrm.2022.100911","journal-title":"Cell Rep Med"},{"issue":"1","key":"581_CR3","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1186\/s13045-022-01278-4","volume":"15","author":"H Xu","year":"2022","unstructured":"Xu H, Jiao D, Liu A, et al. Tumor organoids: applications in cancer modeling and potentials in precision medicine. J Hematol Oncol. 2022;15(1):58. https:\/\/doi.org\/10.1186\/s13045-022-01278-4","journal-title":"J Hematol Oncol"},{"issue":"1","key":"581_CR4","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1186\/s40364-022-00356-6","volume":"10","author":"MA Foo","year":"2022","unstructured":"Foo MA, You M, Chan SL, et al. Clinical translation of patient-derived tumour organoids- bottlenecks and strategies. Biomark Res. 2022;10(1):10. https:\/\/doi.org\/10.1186\/s40364-022-00356-6","journal-title":"Biomark Res"},{"issue":"4","key":"581_CR5","doi-asserted-by":"publisher","first-page":"e100300","DOI":"10.15252\/embj.2018100300","volume":"38","author":"N Sachs","year":"2019","unstructured":"Sachs N, Papaspyropoulos A, Zomer-van Ommen DD, et al. Long-term expanding human airway organoids for disease modeling. EMBO J. 2019;38(4):e100300. https:\/\/doi.org\/10.15252\/embj.2018100300","journal-title":"EMBO J"},{"issue":"1","key":"581_CR6","doi-asserted-by":"publisher","first-page":"3991","DOI":"10.1038\/s41467-019-11867-6","volume":"10","author":"M Kim","year":"2019","unstructured":"Kim M, Mun H, Sung CO, et al. Patient-derived lung cancer organoids as in vitro cancer models for therapeutic screening. Nat Commun. 2019;10(1):3991. https:\/\/doi.org\/10.1038\/s41467-019-11867-6","journal-title":"Nat Commun"},{"issue":"10","key":"581_CR7","doi-asserted-by":"publisher","first-page":"4671","DOI":"10.1021\/pr2004743","volume":"10","author":"CJ Yu","year":"2011","unstructured":"Yu CJ, Wang CL, Wang CI, et al. Comprehensive proteome analysis of malignant pleural effusion for lung cancer biomarker discovery by using multidimensional protein identification technology. J Proteome Res. 2011;10(10):4671\u201382. https:\/\/doi.org\/10.1021\/pr2004743","journal-title":"J Proteome Res"},{"issue":"20","key":"581_CR8","doi-asserted-by":"publisher","first-page":"9825","DOI":"10.1158\/0008-5472.Can-07-1064","volume":"67","author":"G Stathopoulos","year":"2007","unstructured":"Stathopoulos G, Kollintza A, Moschos C, et al. Tumor necrosis factor-alpha promotes malignant pleural effusion. Cancer Res. 2007;67(20):9825\u201334. https:\/\/doi.org\/10.1158\/0008-5472.Can-07-1064","journal-title":"Cancer Res"},{"issue":"1","key":"581_CR9","doi-asserted-by":"publisher","first-page":"312","DOI":"10.1186\/s13046-022-02519-7","volume":"41","author":"S Schuth","year":"2022","unstructured":"Schuth S, Le Blanc S, Krieger T, et al. Patient-specific modeling of stroma-mediated chemoresistance of pancreatic cancer using a three-dimensional organoid-fibroblast co-culture system. 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Front Pharmacol. 2023;13:1083017. https:\/\/doi.org\/10.3389\/fphar.2022.1083017","journal-title":"Front Pharmacol"}],"container-title":["Experimental Hematology &amp; Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s40164-024-00581-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s40164-024-00581-9\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s40164-024-00581-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,15]],"date-time":"2024-11-15T08:03:41Z","timestamp":1731657821000},"score":1,"resource":{"primary":{"URL":"https:\/\/ehoonline.biomedcentral.com\/articles\/10.1186\/s40164-024-00581-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,15]]},"references-count":12,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["581"],"URL":"https:\/\/doi.org\/10.1186\/s40164-024-00581-9","relation":{},"ISSN":["2162-3619"],"issn-type":[{"value":"2162-3619","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,15]]},"assertion":[{"value":"13 September 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 November 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 November 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The study methods with the patient-derived tissues were approved by the Ethics Committee of Affiliated Zhongshan Hospital of Dalian University (2022020-1) and conformed to the standards set by the Declaration of Helsinki. All the experiments were undertaken with the written consent of each patient.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"All authors approve the final form of the manuscript.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"115"}}