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Given the immense diversity of environmental microbiomes, selecting appropriate partners from such a vast microbial pool poses a critical challenge for host organisms. To meet this challenge, hosts have evolved sophisticated internal partner-choice mechanisms that ensure stable associations with beneficial microbes. However, because these symbionts primarily inhabit external environments, environmental conditions themselves are also expected to influence the establishment of symbiosis. Despite this expectation, the mechanistic role of external environmental filters in shaping the intended symbiosis remains largely unexplored. Focusing on stink bugs, which acquire their symbiotic bacteria from soil each generation, we investigated how soil properties influence the establishment of gut symbiosis in terrestrial insects.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Microbiome analyses confirmed that\n                      <jats:italic>Burkholderia<\/jats:italic>\n                      sensu lato overwhelmingly dominates a specific gut organ in six stink bug species from the superfamilies Coreoidea and Lygaeoidea, including serious agricultural pests (relative abundance ranging from 74.5 to 100%). Rearing experiments with isolated\n                      <jats:italic>Burkholderia<\/jats:italic>\n                      revealed that insects were strictly dependent on this symbiont; failure to acquire it from soil severely reduced host growth and reproduction, indicating that the availability of symbionts from soil can represent an ecological constraint. Field surveys identified patches of exceptionally high stink bug density in weedy fields with soil pH\u2009&lt;\u20097.0, whereas such aggregations were absent in fields with pH\u2009\u2265\u20097.0. Laboratory experiments with collected field soils showed that the abundance of\n                      <jats:italic>Burkholderia<\/jats:italic>\n                      in soils was negatively correlated with soil pH, and stink bugs readily acquired their symbionts from soils with pH\u2009&lt;\u20097.0 but rarely from soils with pH\u2009\u2265\u20097.0. Experimental manipulations of soil pH followed by rearing experiments confirmed that increasing soil pH to 7\u20138 markedly suppressed symbiont acquisition by the host, likely by impairing symbiont growth and motility.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      We demonstrate that, beyond host-intrinsic mechanisms, a soil chemical property can act as an externally filter that constrains symbiont acquisition prior to colonization inside the host in a stink bug\u2013\n                      <jats:italic>Burkholderia<\/jats:italic>\n                      symbiosis. This finding highlights how local environmental conditions can shape the assembly of environmentally acquired insect\u2013microbe symbioses.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s40168-026-02402-z","type":"journal-article","created":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T23:03:32Z","timestamp":1778108612000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Soil pH as an external filter shaping stink bug\u2013Burkholderia gut symbiosis"],"prefix":"10.1186","volume":"14","author":[{"given":"Hideomi","family":"Itoh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Shimoji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Nakane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seonghan","family":"Jang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshitomo","family":"Kikuchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,5,7]]},"reference":[{"issue":"2","key":"2402_CR1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01395-15","volume":"7","author":"E Rosenberg","year":"2016","unstructured":"Rosenberg E, Zilber-Rosenberg I. 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