{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T15:00:21Z","timestamp":1778684421509,"version":"3.51.4"},"reference-count":80,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2015,8,8]],"date-time":"2015-08-08T00:00:00Z","timestamp":1438992000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2015,8,8]],"date-time":"2015-08-08T00:00:00Z","timestamp":1438992000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["In Silico Pharmacol."],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Purpose<\/jats:title>\n                <jats:p>Ebola virus (EBOV) is such kind of virus which is responsible for 23,825 cases and 9675 deaths worldwide only in 2014 and with an average diseases fatality rate between 25 % and 90\u00a0%. Although, medical technology has tried to handle the problems, there is no Food and Drug Administration (FDA)-approved therapeutics or vaccines available for the prevention, post exposure, or treatment of Ebola virus disease (EVD).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>In the present study, we used the immunoinformatics approach to design a potential epitope-based vaccine against the RNA-dependent RNA polymerase-L of EBOV. BioEdit v7.2.3 sequence alignment editor, Jalview v2 and CLC Sequence Viewer v7.0.2 were used for the initial sequence analysis for securing the conservancy from the sequences. Later the Immune Epitope Database and Analysis Resource (IEDB-AR) was used for the identification of T-cell and B-cellepitopes associated with type I and II major histocompatibility complex molecules analysis. Finally, the population coverage analysis was employed.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The core epitope \u201cFRYEFTAPF\u201d was found to be the most potential one, with 100 % conservancy among all the strains of EBOV. It also interacted with both type I and II major histocompatibility complex molecules and is considered as nonallergenic in nature. Finally, with impressive cumulative population coverage of 99.87 % for the both MHC-I and MHC-II class throughout the world population was found for the proposed epitope.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>To end, the projected peptide gave us a solid stand to propose for vaccine consideration and that might be experimented for its potency in eliciting immunity through humoral and cell mediated immune responses <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s40203-015-0011-4","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T10:10:02Z","timestamp":1438942202000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Highly conserved regions in Ebola virus RNA dependent RNA polymerase may be act as a universal novel peptide vaccine target: a computational approach"],"prefix":"10.1186","volume":"3","author":[{"given":"Arafat Rahman","family":"Oany","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tahmina","family":"Sharmin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Afrin Sultana","family":"Chowdhury","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tahmina Pervin","family":"Jyoti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Md. Anayet","family":"Hasan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2015,8,8]]},"reference":[{"issue":"1","key":"11_CR1","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1093\/nar\/gkh131","volume":"32","author":"R Apweiler","year":"2004","unstructured":"Apweiler R, Bairoch A, Wu CH et al (2004) UniProt: the universal protein knowledgebase. Nucleic Acids Res 32(1):115\u2013119","journal-title":"Nucleic Acids Res"},{"issue":"2","key":"11_CR2","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1093\/bioinformatics\/bti770","volume":"22","author":"K Arnold","year":"2006","unstructured":"Arnold K, Bordoli L, Kopp J et al (2006) The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling. Bioinformatics 22(2):195\u2013201","journal-title":"Bioinformatics"},{"issue":"8","key":"11_CR3","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1016\/j.autrev.2005.04.005","volume":"4","author":"R Bacchetta","year":"2005","unstructured":"Bacchetta R, Gregori S, Roncarolo M-G (2005) CD4+ regulatory T cells: mechanisms of induction and effector function. Autoimmun Rev 4(8):491\u2013496","journal-title":"Autoimmun Rev"},{"issue":"15","key":"11_CR4","doi-asserted-by":"publisher","first-page":"1418","DOI":"10.1056\/NEJMoa1404505","volume":"371","author":"S Baize","year":"2014","unstructured":"Baize S, Pannetier D, Oestereich L et al (2014) Emergence of Zaire Ebola virus disease in Guinea. N Engl J Med 371(15):1418\u20131425","journal-title":"N Engl J Med"},{"issue":"3","key":"11_CR5","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1093\/bioinformatics\/btq662","volume":"27","author":"P Benkert","year":"2011","unstructured":"Benkert P, Biasini M, Schwede T (2011) Toward the estimation of the absolute quality of individual protein structure models. Bioinformatics 27(3):343\u2013350","journal-title":"Bioinformatics"},{"issue":"23","key":"11_CR6","doi-asserted-by":"publisher","first-page":"3195","DOI":"10.1016\/j.vaccine.2004.02.005","volume":"22","author":"M Bhasin","year":"2004","unstructured":"Bhasin M, Raghava G (2004) Prediction of CTL epitopes using QM, SVM and ANN techniques. Vaccine 22(23):3195\u20133204","journal-title":"Vaccine"},{"issue":"5","key":"11_CR7","doi-asserted-by":"publisher","first-page":"552","DOI":"10.1191\/1352458505ms1225oa","volume":"11","author":"DN Bourdette","year":"2005","unstructured":"Bourdette DN, Edmonds E, Smith C et al (2005) A highly immunogenic trivalent T cell receptor peptide vaccine for multiple sclerosis. Mult Scler 11(5):552\u2013561","journal-title":"Mult Scler"},{"issue":"7","key":"11_CR8","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1186\/1471-2105-7-153","volume":"17","author":"HH Bui","year":"2006","unstructured":"Bui HH, Sidney J, Dinh K et al (2006) Predicting population coverage of T-cell epitope-based diagnostics and vaccines. BMC Bioinformatics 17(7):153","journal-title":"BMC Bioinformatics"},{"issue":"1","key":"11_CR9","doi-asserted-by":"publisher","first-page":"361","DOI":"10.1186\/1471-2105-8-361","volume":"8","author":"HH Bui","year":"2007","unstructured":"Bui HH, Sidney J, Li W et al (2007) Development of an epitope conservancy analysis tool to facilitate the design of epitope-based diagnostics and vaccines. BMC Bioinformatics 8(1):361","journal-title":"BMC Bioinformatics"},{"issue":"6","key":"11_CR10","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1007\/s40259-013-0046-1","volume":"27","author":"JH Choi","year":"2013","unstructured":"Choi JH, Croyle MA (2013) Emerging targets and novel approaches to Ebola virus prophylaxis and treatment. BioDrugs 27(6):565\u2013583","journal-title":"BioDrugs"},{"key":"11_CR11","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1146\/annurev.bi.47.070178.001343","volume":"47","author":"PY Chou","year":"1979","unstructured":"Chou PY, Fasman GD (1979) Empirical predictions of protein conformation. Annu Rev Biochem 47:251\u2013276","journal-title":"Annu Rev Biochem"},{"issue":"1","key":"11_CR12","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/j.jtbi.2004.03.006","volume":"229","author":"G Chowell","year":"2004","unstructured":"Chowell G, Hengartner NW, Castillo-Chavez C et al (2004) The basic reproductive number of Ebola and the effects of public health measures: the cases of Congo and Uganda. J Theor Biol 229(1):119\u2013126","journal-title":"J Theor Biol"},{"key":"11_CR13","doi-asserted-by":"publisher","first-page":"210","DOI":"10.1016\/S0042-6822(02)00014-4","volume":"306","author":"S Crary","year":"2003","unstructured":"Crary S, Towner J, Honig J et al (2003) Analysis of the role of predicted RNA secondary structures in Ebola virus replication. Virology 306:210\u2013218","journal-title":"Virology"},{"issue":"1","key":"11_CR14","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1186\/1471-2105-8-4","volume":"8","author":"IA Doytchinova","year":"2007","unstructured":"Doytchinova IA, Flower DR (2007) VaxiJen: a server for prediction of protective antigens, tumour antigens and subunit vaccines. BMC Bioinformatics 8(1):4","journal-title":"BMC Bioinformatics"},{"issue":"3","key":"11_CR15","doi-asserted-by":"publisher","first-page":"117","DOI":"10.4314\/ajcem.v15i3.1","volume":"15","author":"A Elisha","year":"2014","unstructured":"Elisha A, Adegboro B (2014) Ebola virus diseases. Afr J Clin Exp Microbiol 15(3):117\u2013121","journal-title":"Afr J Clin Exp Microbiol"},{"issue":"3","key":"11_CR16","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1128\/jvi.55.3.836-839.1985","volume":"55","author":"EA Emini","year":"1985","unstructured":"Emini EA, Hughes JV, Perlow DS et al (1985) Induction of hepatitis A virus-neutralizing antibody by a virus-specific synthetic peptide. J Virol 55(3):836\u2013839","journal-title":"J Virol"},{"issue":"9972","key":"11_CR17","doi-asserted-by":"publisher","first-page":"945","DOI":"10.1016\/S0140-6736(15)60179-9","volume":"385","author":"DK Evans","year":"2015","unstructured":"Evans DK, Popova A (2015) West African Ebola crisis and orphans. Lancet 385(9972):945\u2013946","journal-title":"Lancet"},{"key":"11_CR18","first-page":"159","volume":"15","author":"H Feldmann","year":"1999","unstructured":"Feldmann H, Volchkov VE, Volchkova VA et al (1999) The glycoproteins of Marburg and Ebola virus and their potential roles in pathogenesis. Arch Virol 15:159\u2013169","journal-title":"Arch Virol"},{"key":"11_CR19","doi-asserted-by":"crossref","first-page":"2839","DOI":"10.1099\/0022-1317-82-12-2839","volume":"82","author":"H Feldmann","year":"2001","unstructured":"Feldmann H, Volchkov V, Str\u00f6her U et al (2001) Biosynthesis and role of filovirus glycoprotein. J Gen Virol 82:2839\u20132848","journal-title":"J Gen Virol"},{"issue":"8","key":"11_CR20","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1038\/nri1154","volume":"3","author":"H Feldmann","year":"2003","unstructured":"Feldmann H, Jones S, Klenk HD et al (2003) Ebola virus: from discovery to vaccine. Nat Rev Immunol 3(8):677\u2013685","journal-title":"Nat Rev Immunol"},{"key":"11_CR21","doi-asserted-by":"publisher","first-page":"1753","DOI":"10.1110\/ps.9.9.1753","volume":"9","author":"A Fiser","year":"2000","unstructured":"Fiser A, Do RK, Sali A (2000) Modeling of loops in protein structures. Protein Sci 9:1753\u20131773","journal-title":"Protein Sci"},{"issue":"13","key":"11_CR22","doi-asserted-by":"publisher","first-page":"1177","DOI":"10.1056\/NEJMp1409903","volume":"371","author":"TR Frieden","year":"2014","unstructured":"Frieden TR, Damon I, Bell BP et al (2014) Ebola 2014\u2014new challenges, new global response and responsibility. N Engl J Med 371(13):1177\u20131180","journal-title":"N Engl J Med"},{"key":"11_CR23","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1146\/annurev.immunol.17.1.369","volume":"17","author":"KC Garcia","year":"1999","unstructured":"Garcia KC, Teyton L, Wilson IA (1999) Structural basis of T cell recognition. Annu Rev Immunol 17:369\u2013397","journal-title":"Annu Rev Immunol"},{"issue":"2","key":"11_CR24","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1093\/nar\/gkn211","volume":"36","author":"M Garcia-Boronat","year":"2008","unstructured":"Garcia-Boronat M, Diez-Rivero CM, Reinherz EL et al (2008) PVS: a web server for protein sequence variability analysis tuned to facilitate conserved epitope discovery. Nucleic Acids Res 36(2):35\u201341","journal-title":"Nucleic Acids Res"},{"issue":"8","key":"11_CR25","doi-asserted-by":"publisher","first-page":"1619","DOI":"10.1099\/vir.0.067199-0","volume":"95","author":"D Gatherer","year":"2014","unstructured":"Gatherer D (2014) The 2014 Ebola virus disease outbreak in West Africa. J Gen Virol 95(8):1619\u20131624","journal-title":"J Gen Virol"},{"issue":"14","key":"11_CR26","doi-asserted-by":"publisher","first-page":"7296","DOI":"10.1128\/JVI.00561-09","volume":"83","author":"TW Geisbert","year":"2009","unstructured":"Geisbert TW, Geisbert JB, Leung A et al (2009) Single-injection vaccine protects nonhuman primates against infection with marburg virus and three species of ebola virus. J Virol 83(14):7296\u20137304","journal-title":"J Virol"},{"issue":"2","key":"11_CR27","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1016\/0092-8674(94)90336-0","volume":"76","author":"RN Germain","year":"1994","unstructured":"Germain RN (1994) MHC-dependent antigen processing and peptide presentation: providing ligands for T lymphocyte activation. Cell 76(2):287\u2013299","journal-title":"Cell"},{"issue":"9","key":"11_CR28","first-page":"442","volume":"72","author":"M Goeijenbier","year":"2014","unstructured":"Goeijenbier M, van Kampen JJ, Reusken CB (2014) Ebola virus disease: a review on epidemiology, symptoms, treatment and pathogenesis. Neth J Med 72(9):442\u2013448","journal-title":"Neth J Med"},{"key":"11_CR29","first-page":"95","volume":"41","author":"TA Hall","year":"1999","unstructured":"Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT. Nucleic Acids Symp Ser 41:95\u201398","journal-title":"Nucleic Acids Symp Ser"},{"key":"11_CR30","doi-asserted-by":"publisher","first-page":"347","DOI":"10.4137\/BBI.S13402","volume":"7","author":"MA Hasan","year":"2013","unstructured":"Hasan MA, Hossain M, Alam MJ (2013) A computational assay to design an epitope-based Peptide vaccine against Saint Louis encephalitis virus. Bioinform Biol Insights 7:347\u2013355","journal-title":"Bioinform Biol Insights"},{"key":"11_CR31","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1016\/j.molimm.2014.12.013","volume":"65","author":"MA Hasan","year":"2015","unstructured":"Hasan MA, Khan MA, Datta A et al (2015) A comprehensive immunoinformatics and target site study revealedthe corner-stone toward Chikungunya virus treatment. Mol Immunol 65:189\u2013204","journal-title":"Mol Immunol"},{"issue":"4","key":"11_CR32","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1177\/1043659605278935","volume":"16","author":"BL Hewlett","year":"2005","unstructured":"Hewlett BL, Hewlett BS (2005) Providing care and facing death: nursing during Ebola outbreaks in central Africa. J Transcult Nurs 16(4):289\u2013297","journal-title":"J Transcult Nurs"},{"issue":"7","key":"11_CR33","doi-asserted-by":"publisher","first-page":"859","DOI":"10.1517\/14712598.2012.685152","volume":"12","author":"T Hoenen","year":"2012","unstructured":"Hoenen T, Groseth A, Feldmann H (2012) Current Ebola vaccines. Expert Opin Biol Ther 12(7):859\u2013872","journal-title":"Expert Opin Biol Ther"},{"issue":"1","key":"11_CR34","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1023\/A:1007989407305","volume":"16","author":"J Holland","year":"1998","unstructured":"Holland J, Domingo E (1998) Origin and evolution of viruses. Virus Genes 16(1):13\u201321","journal-title":"Virus Genes"},{"key":"11_CR35","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s00251-008-0341-z","volume":"61","author":"I Hoof","year":"2009","unstructured":"Hoof I, Peters B, Sidney J et al (2009) NetMHCpan, a method for MHC class I binding prediction beyond humans. Immunogenetics 61:1\u201313","journal-title":"Immunogenetics"},{"issue":"1","key":"11_CR36","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1586\/14760584.3.1.23","volume":"3","author":"JU Igietseme","year":"2004","unstructured":"Igietseme JU, Eko FO, He Q, Black CM (2004) Antibody regulation of T-cell immunity: implications for vaccine strategies against intracellular pathogens. Expert Rev Vaccines 3(1):23\u201334","journal-title":"Expert Rev Vaccines"},{"issue":"7450","key":"11_CR37","doi-asserted-by":"publisher","first-page":"1229","DOI":"10.1136\/bmj.38069.518137.F6","volume":"328","author":"J Keiser","year":"2004","unstructured":"Keiser J, Utzinger J, Tanner M, Singer BH (2004) Representation of authors and editors from countries with different human development indexes in the leading literature on tropical medicine: survey of current evidence. BMJ 328(7450):1229\u20131232","journal-title":"BMJ"},{"issue":"1","key":"11_CR38","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1016\/j.molimm.2014.04.009","volume":"61","author":"MK Khan","year":"2014","unstructured":"Khan MK, Zaman S, Chakraborty S et al (2014) In silico predicted mycobacterial epitope elicits in vitro T-cell responses. Mol Immunol 61(1):16\u201322","journal-title":"Mol Immunol"},{"issue":"17","key":"11_CR39","doi-asserted-by":"publisher","first-page":"11457","DOI":"10.1128\/JVI.79.17.11457-11466.2005","volume":"79","author":"RS Klein","year":"2005","unstructured":"Klein RS, Lin E, Zhang B et al (2005) Neuronal CXCL10 directs CD8+ T-cell recruitment and control of West Nile virus encephalitis. J Virol 79(17):11457\u201311466","journal-title":"J Virol"},{"issue":"1\u20132","key":"11_CR40","doi-asserted-by":"publisher","first-page":"172","DOI":"10.1016\/0014-5793(90)80535-Q","volume":"276","author":"AS Kolaskar","year":"1990","unstructured":"Kolaskar AS, Tongaonkar PC (1990) A semi-empirical method for prediction of antigenic determinants on protein antigens. FEBS Lett 276(1\u20132):172\u2013174","journal-title":"FEBS Lett"},{"issue":"12","key":"11_CR41","doi-asserted-by":"publisher","first-page":"2083","DOI":"10.1007\/s00705-010-0814-x","volume":"155","author":"JH Kuhn","year":"2010","unstructured":"Kuhn JH, Becker S, Ebihara H et al (2010) Proposal for a revised taxonomy of the family Filoviridae: classification, names of taxa and viruses, and virus abbreviations. Arch Virol 155(12):2083\u20132103","journal-title":"Arch Virol"},{"issue":"1","key":"11_CR42","doi-asserted-by":"publisher","first-page":"424","DOI":"10.1186\/1471-2105-8-424","volume":"8","author":"MV Larsen","year":"2007","unstructured":"Larsen MV, Lundegaard C, Lamberth K et al (2007) Large-scale validation of methods for cytotoxic T-lymphocyte epitope prediction. BMC Bioinformatics 8(1):424","journal-title":"BMC Bioinformatics"},{"issue":"4","key":"11_CR43","doi-asserted-by":"publisher","first-page":"477","DOI":"10.1007\/BF00228148","volume":"8","author":"RA Laskowski","year":"1996","unstructured":"Laskowski RA, Rullmann JAC, MacArthur MW et al (1996) AQUA and PROCHECK-NMR: programs for checking the quality of protein structures solved by NMR. J Biomol NMR 8(4):477\u2013486","journal-title":"J Biomol NMR"},{"issue":"5656","key":"11_CR44","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1126\/science.1092528","volume":"303","author":"EM Leroy","year":"2004","unstructured":"Leroy EM, Rouquet P, Formenty P et al (2004) Multiple Ebola virus transmission events and rapid decline of central African wildlife. Science 303(5656):387\u2013390","journal-title":"Science"},{"issue":"06","key":"11_CR45","doi-asserted-by":"publisher","first-page":"1138","DOI":"10.1017\/S0950268813002215","volume":"142","author":"YH Li","year":"2014","unstructured":"Li YH, Chen SP (2014) Evolutionary history of Ebola virus. Epidemiol Infect 142(06):1138\u20131145","journal-title":"Epidemiol Infect"},{"issue":"6","key":"11_CR46","doi-asserted-by":"publisher","first-page":"857","DOI":"10.1089\/106652703322756113","volume":"10","author":"L Liao","year":"2003","unstructured":"Liao L, Noble WS (2003) Combining pairwise sequence similarity and support vector machines for detecting remote protein evolutionary and structural relationships. J Comput Biol 10(6):857\u2013868","journal-title":"J Comput Biol"},{"issue":"7","key":"11_CR47","doi-asserted-by":"publisher","first-page":"1989","DOI":"10.1002\/1521-4141(200107)31:7<1989::AID-IMMU1989>3.0.CO;2-M","volume":"31","author":"JA L\u00f3pez","year":"2001","unstructured":"L\u00f3pez JA, Weilenman C, Audran R et al (2001) A synthetic malaria vaccine elicits a potent CD8+ and CD4+ T lymphocyte immune response in humans. Implications for vaccination strategies. Eur J Immunol 31(7):1989\u20131998","journal-title":"Eur J Immunol"},{"issue":"6","key":"11_CR48","doi-asserted-by":"publisher","first-page":"985","DOI":"10.2105\/AJPH.94.6.985","volume":"94","author":"TM McKeever","year":"2004","unstructured":"McKeever TM, Lewis SA, Smith C et al (2004) Vaccination and allergic disease: a birth cohort study. Am J Public Health 94(6):985","journal-title":"Am J Public Health"},{"issue":"6","key":"11_CR49","doi-asserted-by":"publisher","first-page":"5861","DOI":"10.1371\/journal.pone.0005861","volume":"4","author":"HC Muh","year":"2009","unstructured":"Muh HC, Tong JC, Tammi MT (2009) AllerHunter: a SVM-pairwise system for assessment of allergenicity and allergic cross-reactivity in proteins. PLoS One 4(6):5861","journal-title":"PLoS One"},{"key":"11_CR50","doi-asserted-by":"crossref","first-page":"8756","DOI":"10.1128\/JVI.72.11.8756-8764.1998","volume":"72","author":"E M\u00fchlberger","year":"1998","unstructured":"M\u00fchlberger E, Lotfering B, Klenk H-D et al (1998) Three of the four nucleocapsid proteins of Marburg virus, NP, VP35, and L, are sufficient to mediate replication and transcription of Marburg virus-specific monocistronic minigenomes. J Virol 72:8756\u20138764","journal-title":"J Virol"},{"key":"11_CR51","doi-asserted-by":"crossref","first-page":"2333","DOI":"10.1128\/JVI.73.3.2333-2342.1999","volume":"73","author":"E M\u00fchlberger","year":"1999","unstructured":"M\u00fchlberger E, Weik M, Volchkov V et al (1999) Comparison of the transcription and replication strategies of Marburg virus and Ebola virus by using artificial replication systems. J Virol 73:2333\u20132342","journal-title":"J Virol"},{"issue":"5","key":"11_CR52","doi-asserted-by":"publisher","first-page":"2371","DOI":"10.4049\/jimmunol.168.5.2371","volume":"168","author":"DT Nair","year":"2002","unstructured":"Nair DT, Singh K, Siddiqui Z et al (2002) Epitope recognition by diverse antibodies suggests conformational convergence in an antibody response. J Immunol 168(5):2371\u20132382","journal-title":"J Immunol"},{"key":"11_CR53","doi-asserted-by":"publisher","first-page":"796","DOI":"10.1371\/journal.pone.0000796","volume":"2","author":"M Nielsen","year":"2007","unstructured":"Nielsen M, Lundegaard C, Blicher T et al (2007) NetMHCpan, a method for quantitative predictions of peptide binding to any HLA-A and -B locus protein of known sequence. PLoS One 2:796","journal-title":"PLoS One"},{"key":"11_CR54","first-page":"1139","volume":"8","author":"AR Oany","year":"2008","unstructured":"Oany AR, Emran AA, Jyoti TP (2008) Design of an epitope-based peptide vaccine against spike protein of human corona virus: an in silico approach. Drug Des Devel Ther 8:1139\u20131149","journal-title":"Drug Des Devel Ther"},{"key":"11_CR55","doi-asserted-by":"publisher","first-page":"1","DOI":"10.2147\/AABC.S75250","volume":"8","author":"AR Oany","year":"2015","unstructured":"Oany AR, Ahmad SAI, Hossain MU, Jyoti TP (2015) Identification of highly conserved regions in L-segment of Crimean\u2013Congo hemorrhagic fever virus and immunoinformatic prediction about potential novel vaccine. Adv Appl Bioinformatics Chem 8:1\u201310","journal-title":"Adv Appl Bioinformatics Chem"},{"key":"11_CR56","doi-asserted-by":"publisher","first-page":"5425","DOI":"10.1021\/bi00367a013","volume":"25","author":"JM Parker","year":"1986","unstructured":"Parker JM, Guo D, Hodges RS (1986) New hydrophilicity scale derived from high-performance liquid chromatography peptide retention data: correlation of predicted surface residues with antigenicity and X-ray-derived accessible sites. Biochemistry 25:5425\u20135432","journal-title":"Biochemistry"},{"issue":"1","key":"11_CR57","first-page":"ix","volume":"179","author":"CJ Peters","year":"1999","unstructured":"Peters CJ, Peters JW (1999) An introduction to Ebola: the virus and the disease. J Infect Dis 179(1):ix\u2013xvi","journal-title":"J Infect Dis"},{"issue":"1","key":"11_CR58","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1186\/1471-2105-6-132","volume":"6","author":"B Peters","year":"2005","unstructured":"Peters B, Sette A (2005) Generating quantitative models describing the sequence specificity of biological processes with the stabilized matrix method. BMC Bioinformatics 6(1):132","journal-title":"BMC Bioinformatics"},{"issue":"3","key":"11_CR59","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1046\/j.1440-1711.2002.01093.x","volume":"80","author":"N Petrovsky","year":"2002","unstructured":"Petrovsky N, Brusic V (2002) Computational immunology: the coming of age. Immunol Cell Biol 80(3):248\u2013254","journal-title":"Immunol Cell Biol"},{"key":"11_CR60","doi-asserted-by":"publisher","first-page":"1153","DOI":"10.1099\/0022-1317-71-5-1153","volume":"71","author":"O Poch","year":"1990","unstructured":"Poch O, Blumberg BM, Bougueleret L et al (1990) Sequence comparison of five polymerases (L proteins) of unsegmented negative-strand RNA viruses: theoretical assignment of functional domains. J Gen Virol 71:1153\u20131162","journal-title":"J Gen Virol"},{"issue":"5","key":"11_CR61","doi-asserted-by":"publisher","first-page":"837","DOI":"10.2217\/pgs.09.25","volume":"10","author":"GA Poland","year":"2009","unstructured":"Poland GA, Ovsyannikova IG, Jacobson RM (2009) Application of pharmacogenomics to vaccines. Pharmacogenomics 10(5):837\u2013852","journal-title":"Pharmacogenomics"},{"issue":"5047","key":"11_CR62","doi-asserted-by":"publisher","first-page":"959","DOI":"10.1126\/science.1546293","volume":"255","author":"JM Rini","year":"1992","unstructured":"Rini JM, Schulze-Gahmen U, Wilson IA (1992) Structural evidence for induced fit as a mechanism for antibody-antigen recognition. Science 255(5047):959\u2013965","journal-title":"Science"},{"key":"11_CR63","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1038\/nm1212","volume":"11","author":"HL Robinson","year":"2005","unstructured":"Robinson HL, Amara RR (2005) T cell vaccines for microbial infections. Nat Med 11:25\u201332","journal-title":"Nat Med"},{"issue":"2","key":"11_CR64","doi-asserted-by":"publisher","first-page":"283","DOI":"10.3201\/eid1102.040533","volume":"11","author":"P Rouquet","year":"2005","unstructured":"Rouquet P, Froment JM, Bermejo M (2005) Wild animal mortality monitoring and human Ebola outbreaks, Gabon and Republic of Congo, 2001\u20132003. Emerg Infect Dis 11(2):283\u2013290","journal-title":"Emerg Infect Dis"},{"key":"11_CR65","doi-asserted-by":"publisher","first-page":"779","DOI":"10.1006\/jmbi.1993.1626","volume":"234","author":"A Sali","year":"1993","unstructured":"Sali A, Blundell TL (1993) Comparative protein modelling by satisfaction of spatial restraints. J Mol Biol 234:779\u2013815","journal-title":"J Mol Biol"},{"issue":"3","key":"11_CR66","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1002\/prot.340230306","volume":"23","author":"A \u0160ali","year":"1995","unstructured":"\u0160ali A, Potterton L, Yuan F et al (1995) Evaluation of comparative protein modeling by MODELLER. Proteins 23(3):318\u2013326","journal-title":"Proteins"},{"issue":"4","key":"11_CR67","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1016\/S0952-7915(03)00083-9","volume":"15","author":"A Sette","year":"2003","unstructured":"Sette A, Fikes J (2003) Epitope-based vaccines: an update on epitope identification, vaccine design and delivery. Curr Opin Immunol 15(4):461\u2013470","journal-title":"Curr Opin Immunol"},{"issue":"6","key":"11_CR68","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1034\/j.1399-0039.2002.590601.x","volume":"59","author":"A Sette","year":"2002","unstructured":"Sette A, Newman M, Livingston B et al (2002) Optimizing vaccine design for cellular processing, MHC binding and TCR recognition. Tissue Antigens 59(6):443\u2013451","journal-title":"Tissue Antigens"},{"issue":"15","key":"11_CR69","doi-asserted-by":"publisher","first-page":"8312","DOI":"10.1128\/JVI.78.15.8312-8321.2004","volume":"78","author":"B Shrestha","year":"2004","unstructured":"Shrestha B, Diamond MS (2004) Role of CD8+ T cells in control of West Nile virus infection. J Virol 78(15):8312\u20138321","journal-title":"J Virol"},{"key":"11_CR70","doi-asserted-by":"publisher","first-page":"605","DOI":"10.1038\/35046108","volume":"408","author":"NJ Sullivan","year":"2000","unstructured":"Sullivan NJ, Sanchez A, Rollin PE, Yang ZY, Nabel GJ (2000) Development of a preventive vaccine for Ebola virus infection in primates. Nature 408:605\u2013609","journal-title":"Nature"},{"issue":"26","key":"11_CR71","doi-asserted-by":"publisher","first-page":"14764","DOI":"10.1073\/pnas.94.26.14764","volume":"94","author":"A Takada","year":"1997","unstructured":"Takada A, Robison C, Goto H et al (1997) A system for functional analysis of Ebola virus glycoprotein. Proc Natl Acad Sci 94(26):14764\u201314769","journal-title":"Proc Natl Acad Sci"},{"issue":"22","key":"11_CR72","doi-asserted-by":"publisher","first-page":"4673","DOI":"10.1093\/nar\/22.22.4673","volume":"22","author":"JD Thompson","year":"1994","unstructured":"Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22(22):4673\u20134680","journal-title":"Nucleic Acids Res"},{"issue":"2","key":"11_CR73","doi-asserted-by":"publisher","first-page":"398","DOI":"10.1016\/j.virol.2006.10.033","volume":"360","author":"NB Trainor","year":"2007","unstructured":"Trainor NB, Crill WD, Roberson JA et al (2007) Mutation analysis of the fusion domain region of St. Louis encephalitis virus envelope protein. Virology 360(2):398\u2013406","journal-title":"Virology"},{"issue":"1","key":"11_CR74","doi-asserted-by":"publisher","first-page":"122","DOI":"10.1093\/molbev\/msg010","volume":"20","author":"SS Twiddy","year":"2003","unstructured":"Twiddy SS, Holmes EC, Rambaut A (2003) Inferring the rate and time-scale of dengue virus evolution. Mol Biol Evol 20(1):122\u2013129","journal-title":"Mol Biol Evol"},{"issue":"2","key":"11_CR75","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1099\/0022-1317-80-2-355","volume":"80","author":"VE Volchkov","year":"1999","unstructured":"Volchkov VE et al (1999) Characterization of the L gene and 5\u2032trailer region of Ebola virus. J Gen Virol 80(2):355\u2013362","journal-title":"J Gen Virol"},{"issue":"4","key":"11_CR76","doi-asserted-by":"publisher","first-page":"e1000048","DOI":"10.1371\/journal.pcbi.1000048","volume":"4","author":"P Wang","year":"2008","unstructured":"Wang P, Sidney J, Dow C et al (2008) A systematic assessment of MHC class II peptide binding predictions and evaluation of a consensus approach. PLoS Comput Biol 4(4):e1000048","journal-title":"PLoS Comput Biol"},{"issue":"1","key":"11_CR77","doi-asserted-by":"publisher","first-page":"568","DOI":"10.1186\/1471-2105-11-568","volume":"11","author":"P Wang","year":"2010","unstructured":"Wang P, Sidney J, Kim Y et al (2010) Peptide binding predictions for HLA DR, DP and DQ molecules. BMC Bioinformatics 11(1):568","journal-title":"BMC Bioinformatics"},{"issue":"13","key":"11_CR78","doi-asserted-by":"publisher","first-page":"2138","DOI":"10.1093\/bioinformatics\/bth195","volume":"20","author":"JJ Ward","year":"2004","unstructured":"Ward JJ, McGuffin LJ, Bryson K et al (2004) The DISOPRED server for the prediction of protein disorder. Bioinformatics 20(13):2138\u20132139","journal-title":"Bioinformatics"},{"issue":"9","key":"11_CR79","doi-asserted-by":"publisher","first-page":"1189","DOI":"10.1093\/bioinformatics\/btp033","volume":"25","author":"AM Waterhouse","year":"2009","unstructured":"Waterhouse AM, Procter JB, Martin DM et al (2009) Jalview version 2 \u2013 a multiple sequence alignment editor and analysis workbench. Bioinformatics 25(9):1189\u20131191","journal-title":"Bioinformatics"},{"issue":"10","key":"11_CR80","doi-asserted-by":"publisher","first-page":"5611","DOI":"10.4049\/jimmunol.171.10.5611","volume":"171","author":"CC Wilson","year":"2003","unstructured":"Wilson CC, McKinney D, Anders M et al (2003) Development of a DNA vaccine designed to induce cytotoxic T lymphocyte responses to multiple conserved epitopes in HIV-1. J Immunol 171(10):5611\u20135623","journal-title":"J Immunol"}],"container-title":["In Silico Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s40203-015-0011-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s40203-015-0011-4\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s40203-015-0011-4","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s40203-015-0011-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,17]],"date-time":"2022-11-17T16:15:05Z","timestamp":1668701705000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1186\/s40203-015-0011-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,8,8]]},"references-count":80,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2015,12]]}},"alternative-id":["11"],"URL":"https:\/\/doi.org\/10.1186\/s40203-015-0011-4","relation":{},"ISSN":["2193-9616"],"issn-type":[{"value":"2193-9616","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,8,8]]},"assertion":[{"value":"7 April 2015","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 July 2015","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 August 2015","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"7"}}