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In this study, the epidemiological characteristics of human brucellosis were analyzed, and the genotypic profile and antimicrobial susceptibilities of\n                      <jats:italic>Brucella melitensis<\/jats:italic>\n                      strains isolated from humans in Hinggan League were investigated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The epidemic characteristics were described using case number, constituent ratio, and rate. The 418 human blood samples were collected and tested by bacteriology, and suspect colonies were isolated and identified by conventional biotyping assays, the VITEK 2.0 microbial identification system, and AMOS (\n                      <jats:italic>Brucella abortus<\/jats:italic>\n                      ,\n                      <jats:italic>B. melitensis<\/jats:italic>\n                      ,\n                      <jats:italic>B. ovis<\/jats:italic>\n                      , and\n                      <jats:italic>B. suis<\/jats:italic>\n                      )-PCR. Subsequently, all strains were genotyped using multiple-locus variable-number tandem repeat analysis (MLVA) assays, and the antimicrobial susceptibility pattern of\n                      <jats:italic>Brucella<\/jats:italic>\n                      strains against the 10 most commonly used antibiotics was determined by microdilution method.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 22\u2009848 cases of human brucellosis were reported from 2004 to 2019, with an annual average incidence of 87.2\/100\u00a0000. The incidence rates in developed areas of animal husbandry (Horqin Youyi Qianqi [161.2\/100\u00a0000] and Horqin Youyi Zhongqi [112.1\/100\u00a0000]) were significantly higher than those in forest areas (Arxan [19.2\/100\u00a0000]) (\n                      <jats:italic>\u03c7<\/jats:italic>\n                      <jats:sup>2<\/jats:sup>\n                      \u2009=\u200932.561,\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.001). In addition, peak morbidity occurred during May\u2013August, accounting for 72.6% (16582\/22\u00a0848) of cases. The highest number of cases occurred in the 40+ age group, accounting for 44.4% (10\u2009137\/22484) of cases, and morbidity in males was significantly higher than that in females in all age groups (\u03c7\n                      <jats:sup>2<\/jats:sup>\n                      \u2009=\u2009299.97,\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.001), the most common occupation was farmers. A total of 54\n                      <jats:italic>B. melitensis<\/jats:italic>\n                      strains were divided into 37 genotypes (GT1\u201337) with 80\u2013100% genetic similarity. All 25 strains were sensitive to seven tested antibiotics, phenotypic resistance to cotrimoxazole and azithromycin was observed in 5 (20%) and 25 (100%) of the isolates, respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Human brucellosis exhibited a significant increasing trend and\n                      <jats:italic>B. melitensis<\/jats:italic>\n                      is the main pathogen responsible for human brucellosis in this region. Improved surveillance of infected animals (sheep) and limiting their transfer and trade are optional strategies for decreasing the incidence of this disease.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s40249-020-00697-0","type":"journal-article","created":{"date-parts":[[2020,6,29]],"date-time":"2020-06-29T08:04:05Z","timestamp":1593417845000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Epidemiologically characteristics of human brucellosis and antimicrobial susceptibility pattern of Brucella melitensis in Hinggan League of the Inner Mongolia Autonomous Region, China"],"prefix":"10.1186","volume":"9","author":[{"given":"Hai-Tao","family":"Yuan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng-Ling","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4904-4878","authenticated-orcid":false,"given":"Li-Na","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen-Jun","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-Guo","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,6,29]]},"reference":[{"issue":"1","key":"697_CR1","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1186\/s12864-018-4762-2","volume":"19","author":"SV Pisarenko","year":"2018","unstructured":"Pisarenko SV, Kovalev DA, Volynkina AS, Ponomarenko DG, Rusanova DV, Zharinova NV, et al. 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The study protocol was approved by the Ethics Committees of the Hinggan League Central for Disease Control and Prevention. Informed consent was obtained from all of the patients prior to diagnosis. <i>Brucella<\/i> spp. was isolated from patients\u2019 blood samples following confirmation of their consent.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Written informed consent for publication was obtained from all participants.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"79"}}