{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T02:27:09Z","timestamp":1774924029105,"version":"3.50.1"},"reference-count":10,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2020,3,2]],"date-time":"2020-03-02T00:00:00Z","timestamp":1583107200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,3,2]],"date-time":"2020-03-02T00:00:00Z","timestamp":1583107200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"The National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["81420108024"],"award-info":[{"award-number":["81420108024"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"The National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["31830087"],"award-info":[{"award-number":["31830087"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000009","name":"Foundation for the National Institutes of Health","doi-asserted-by":"publisher","award":["AI136850"],"award-info":[{"award-number":["AI136850"]}],"id":[{"id":"10.13039\/100000009","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2014A030312016"],"award-info":[{"award-number":["2014A030312016"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Guangzhou Synergy Innovation Key Program for Health","award":["201803040006"],"award-info":[{"award-number":["201803040006"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Infect Dis Poverty"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n<jats:title>Background<\/jats:title>\n<jats:p>Dengue is a re-emerging public health problem and mosquito-borne infectious disease that is transmitted mainly by <jats:italic>Aedes aegypti<\/jats:italic> and <jats:italic>Ae. albopictus<\/jats:italic>. Early diagnosis, isolation, and treatment of patients are critical steps for dengue epidemic control, especially to prevent secondary transmission of dengue virus (DENV). However, little is known about defervescent dengue patients as a source of infection.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Methods<\/jats:title>\n<jats:p>This case study describes 1268 dengue patients hospitalized at Guangzhou Eighth People\u2019s Hospital from June 2013 to December 2014. The viral loads of each individual were measured using real-time reverse transcription-polymerase chain reaction. <jats:italic>Ae. aegypti<\/jats:italic> and <jats:italic>Ae. albopictus<\/jats:italic> were exposed to blood meal with gradated dengue viral loads to characterize the relationship between viremia in dengue patients and the vector competence of vector mosquitoes.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>The viral numbers in the blood were measured, ranging from 10<jats:sup>8<\/jats:sup> to 10<jats:sup>3<\/jats:sup> copies\/ml from day 1 to day 12 after fever onset. Vector competence analysis of <jats:italic>Ae. aegypti<\/jats:italic> and <jats:italic>Ae. albopictus<\/jats:italic> indicated that viremia &gt;\u200910<jats:sup>4<\/jats:sup> copies\/ml can still infect vector mosquitoes, which implied that the defervescent dengue patients might be a source of infection.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusions<\/jats:title>\n<jats:p>The results of this study indicate that some defervescent dengue patients still have sufficient viral load to infect vector mosquitoes. Therefore, the protection against mosquito biting for these people should be extended to prevent secondary transmission events.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s40249-020-0631-8","type":"journal-article","created":{"date-parts":[[2020,3,2]],"date-time":"2020-03-02T00:02:26Z","timestamp":1583107346000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Defervescent dengue patients might be a potential source of infection for vector mosquitoes"],"prefix":"10.1186","volume":"9","author":[{"given":"Ye","family":"Xu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling-Zhai","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya-Zhou","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin-Bao","family":"Gu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kun","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi-Qiang","family":"Peng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Hong","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fu-Chun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9134-1860","authenticated-orcid":false,"given":"Xiao-Guang","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,3,2]]},"reference":[{"issue":"7446","key":"631_CR1","doi-asserted-by":"publisher","first-page":"504","DOI":"10.1038\/nature12060","volume":"496","author":"S Bhatt","year":"2013","unstructured":"Bhatt S, Gething PW, Brady OJ, Messina JP, Farlow AW, Moyes CL, et al. The global distribution and burden of dengue. Nature. 2013;496(7446):504.","journal-title":"Nature"},{"issue":"8","key":"631_CR2","doi-asserted-by":"publisher","first-page":"e1760","DOI":"10.1371\/journal.pntd.0001760","volume":"6","author":"OJ Brady","year":"2012","unstructured":"Brady OJ, Gething PW, Bhatt S, Messina JP, Brownstein JS, Hoen AG, et al. Refining the global spatial limits of dengue virus transmission by evidence-based consensus. PLoS Negl Trop Dis. 2012;6(8):e1760.","journal-title":"PLoS Negl Trop Dis"},{"issue":"22","key":"631_CR3","doi-asserted-by":"publisher","first-page":"9072","DOI":"10.1073\/pnas.1303395110","volume":"110","author":"NM Nguyen","year":"2013","unstructured":"Nguyen NM, Kien DTH, Tuan TV, Quyen NTH, Tran CN, Thi LV, et al. Host and viral features of human dengue cases shape the population of infected and infectious Aedes aegypti mosquitoes. 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Guidelines for diagnosis and treatment of dengue. 2014. http:\/\/www.nhc.gov.cn\/yzygj\/s3593g\/201410\/d417aa2e783949e48f8a7366d7fdfacc.shtml.\u00a0Accessed 12 July 2019."},{"issue":"7","key":"631_CR7","doi-asserted-by":"publisher","first-page":"1085","DOI":"10.3201\/eid2307.161528","volume":"23","author":"Z Liu","year":"2017","unstructured":"Liu Z, Zhou T, Lai Z, Zhang Z, Jia Z, Zhou G, et al. Competence of Aedes aegypti, Ae. albopictus, and Culex quinquefasciatus mosquitoes as Zika virus vectors, China. Emerg Infect Dis. 2017;23(7):1085.","journal-title":"Emerg Infect Dis"},{"issue":"10","key":"631_CR8","doi-asserted-by":"publisher","first-page":"e3188","DOI":"10.1371\/journal.pntd.0003188","volume":"8","author":"JP Ledermann","year":"2014","unstructured":"Ledermann JP, Guillaumot L, Yug L, Saweyog SC, Tided M, Machieng P, et al. Aedes hensilli as a potential vector of Chikungunya and Zika viruses. 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