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However, <jats:italic>Brucella<\/jats:italic> species in humans have not been investigated and the origin of the disease remains poorly known in wide parts of \u0421entral Asia. Thus, molecular characterization of the circulating strains is a critical first step in understanding <jats:italic>Brucella<\/jats:italic> diversity in the country.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>In this study, isolates were collected from patients with suspected brucellosis from different regions in Kyrgyzstan between 2019 and 2020. The detection and identification of <jats:italic>Brucella<\/jats:italic> was carried out by Bruce-ladder PCR. Next generation sequencing was used to sequence the 89 <jats:italic>Brucella<\/jats:italic> isolates, which were genotyped by cgSNP and cgMLST to identify epidemiological connection between <jats:italic>Brucella<\/jats:italic> isolates as well as placing them in the context of the global <jats:italic>Brucella<\/jats:italic> phylogeny.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The <jats:italic>Brucella<\/jats:italic> strains isolated from all regions of Kyrgyzstan were identified as <jats:italic>B. melitensis.<\/jats:italic> Based on cgSNP analysis, 18 sequence types were differentiated. The highest numbers of different sequence types were found in Batken (<jats:italic>n<\/jats:italic>\u2009=\u20098), Osh (<jats:italic>n<\/jats:italic>\u2009=\u20098) and Jalal-Abad (<jats:italic>n<\/jats:italic>\u2009=\u20096) oblasts. According to cgSNP and cgMLST analyses, different <jats:italic>B. melitensis<\/jats:italic> lineages circulate in Kyrgyzstan, all of them belonging to the Eastern Mediterranean group of the global <jats:italic>Brucella<\/jats:italic> phylogeny with the highest similarity to strains from Turkmenistan, Iran and Turkey.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>In the present study, <jats:italic>B. melitensis<\/jats:italic> was identified as a causative agent of human brucellosis in Kyrgyzstan and different lineages could be identified. Since this study focused on isolates of human origin, the identity of <jats:italic>Brucella<\/jats:italic> species and lineages circulating among animal populations remains elusive. Implementing culture techniques and use of most recent molecular, bioinformatic and epidemiological tools are needed to set up a One Health approach to combat brucellosis in Kyrgyzstan. Further, other \u0421entral Asian countries need to take part in this effort as brucellosis is a transboundary disease in these regions.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Graphical Abstract<\/jats:title>\n                \n              <\/jats:sec>","DOI":"10.1186\/s40249-022-01044-1","type":"journal-article","created":{"date-parts":[[2022,12,8]],"date-time":"2022-12-08T12:02:59Z","timestamp":1670500979000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["First record of the human infection of Brucella melitensis in Kyrgyzstan: evidence from whole-genome sequencing-based analysis"],"prefix":"10.1186","volume":"11","author":[{"given":"Kalysbek","family":"Kydyshov","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nurbolot","family":"Usenbaev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stalbek","family":"Berdiev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aigul","family":"Dzhaparova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aziza","family":"Abidova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nuraiym","family":"Kebekbaeva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Murat","family":"Abdyraev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gamal","family":"Wareth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanka","family":"Brangsch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Falk","family":"Melzer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heinrich","family":"Neubauer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mathias W.","family":"Pletz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,12,1]]},"reference":[{"issue":"10","key":"1044_CR1","doi-asserted-by":"publisher","first-page":"e1865","DOI":"10.1371\/journal.pntd.0001865","volume":"6","author":"AS Dean","year":"2012","unstructured":"Dean AS, Crump L, Greter H, Schelling E, Zinsstag J. 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All strains were collected as part of the standard clinical investigation of patients with suspected brucellosis and the strains were anonymized. All <i>Brucella<\/i> strains used in this study were obtained from a collection kept at the Republican Center for Quarantine and Highly Dangerous Infections of the Ministry of Health, Bishkek, Kyrgyzstan.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"Written informed consent was obtained from the patient for the publication of this report and any accompanying images.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed consent"}},{"value":"Not applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing of interests.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"120"}}