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However, there are limitation facing the use of this method including timely availability of the test cassettes. The aim of this study was to determine the reliability of dried urine spot (DUS) method for collection of urine and detection of<jats:italic>S. mansoni<\/jats:italic>using Schisto POC-CCA cassettes in a resource-limited settings.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A cross-sectional study was conducted between October and November 2022 among 250 primary school children in Sengerema District, northwestern Tanzania.<jats:italic>S. mansoni<\/jats:italic>CCA was detected in filter paper-based DUS, liquid urine using DUS Schisto POC-CCA (index), and direct urine Schisto POC-CCA (comparator) methods respectively.<jats:italic>S. mansoni<\/jats:italic>eggs in stool were detected using duplicate Kato-Katz (KK) method. The measures of accuracy were computed and compared between the index and comparator methods. The strength of agreement between inter-raters precisions was tested using Cohen\u2019s kappa (<jats:italic>k<\/jats:italic>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>This study revealed<jats:italic>S. mansoni<\/jats:italic>prevalence rates of 28.8%, 54.0% and 50.8% by duplicate KK, direct urine Schisto POC-CCA and DUS Schisto POC-CCA methods respectively. The mean intensity of infection among infected participants was 86.3 eggs per gram of stool (EPG) ranging from 12.0 EPG to 824.0 EPG. The sensitivity of DUS Schisto POC-CCA and direct urine Schisto POC-CCA was 94.44% (95%<jats:italic>CI<\/jats:italic>: 89.15\u201399.74%) and 97.22% (95%<jats:italic>CI<\/jats:italic>: 93.43\u2013100.00%) respectively. The DUS Schisto POC-CCA method had slightly higher specificity (66.85%) than direct urine Schisto POC-CCA method (63.48%). The accuracy of the DUS Schisto POC-CCA was found to be slightly high (74.80%, 95%<jats:italic>CI<\/jats:italic>: 68.94\u201379.06%) compared to that of direct urine Schisto POC-CCA (73.20%, 95%<jats:italic>CI<\/jats:italic>: 67.25\u201378.59%). There was good agreement between two laboratory technologists who performed the DUS Schisto POC-CCA method on similar samples (<jats:italic>k<\/jats:italic>\u2009=\u20090.80, 95%<jats:italic>CI<\/jats:italic>: 0.59\u20130.95).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The DUS Schisto POC-CCA method had comparable<jats:italic>S. mansoni<\/jats:italic>detection accuracy to direct urine Schisto POC-CCA. This suggests that the method could be a potential alternative to direct urine Schisto POC-CCA for screening<jats:italic>S. mansoni<\/jats:italic>in resource-limited situations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Graphical Abstract<\/jats:title><\/jats:sec>","DOI":"10.1186\/s40249-024-01183-7","type":"journal-article","created":{"date-parts":[[2024,2,18]],"date-time":"2024-02-18T09:02:07Z","timestamp":1708246927000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Accuracy and precision of dried urine spot method for the detection of Schistosoma mansoni circulating cathodic antigens in resource-limited settings"],"prefix":"10.1186","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9380-6158","authenticated-orcid":false,"given":"Abdallah","family":"Zacharia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clemence","family":"Kinabo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Twilumba","family":"Makene","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huda","family":"Omary","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George","family":"Ogweno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faraja","family":"Lyamuya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Billy","family":"Ngasala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,2,18]]},"reference":[{"key":"1183_CR1","unstructured":"Centers for Disease Control and Prevention. 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Permission to conduct the study in the Mwanza region was requested from President Office, Regional Administration and Local Government, Regional Administrative Secretary of Mwanza, and the District Executive Director of Sengerema district. At the beginning of the study, school head teachers were asked to provide written informed consent. Children were asked to provide assent (verbal) after being informed about the study. Children infected with <i>S. mansoni<\/i> by any of the diagnostic tests were referred to the nearby health facility for treatment.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"15"}}