{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T03:00:45Z","timestamp":1780282845826,"version":"3.54.0"},"reference-count":32,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Infect Dis Poverty"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Human papillomavirus (HPV)-associated anal squamous cell carcinoma (SCC) is strongly linked to persistent infection with high-risk HPV genotypes, particularly in people living with human immunodeficiency virus (HIV), who experience higher rates of viral persistence and oncogenic progression. The burden of HPV-related malignancies is disproportionately higher in low-resource settings, where limited access to screening, histopathology, and specialist care contributes to delayed diagnosis and advanced-stage presentation. In such contexts, rapid molecular diagnostic tools may help overcome diagnostic delays and support earlier clinical decision-making.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Case presentation<\/jats:title>\n                    <jats:p>\n                      We describe an atypically presenting HPV-associated anal squamous cell carcinoma in a man living with HIV from the Peruvian Amazon, a region characterized by geographic and structural barriers to healthcare access. The patient presented with a large exophytic perianal lesion measuring 16\u2009\u00d7\u20097\u2009\u00d7\u20094\u00a0cm\n                      <jats:sup>3<\/jats:sup>\n                      , exceeding sizes typically reported in the literature and reflecting prolonged disease progression. High-risk HPV type 16 was detected using a GeneXpert\u00ae assay applied to solid tissue, an approach rarely described, as most available evidence and current guidelines focus on swab-based specimens. Histopathological confirmation of HPV-associated squamous cell carcinoma was delayed due to limited local pathology services. In the interim, molecular HPV testing contributed to early diagnostic orientation and clinical planning. Imaging findings raised suspicion for advanced disease, although definitive metastatic staging could not be established due to incomplete diagnostic workup.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This case highlights the potential utility of tissue-based GeneXpert HPV testing as a rapid adjunctive diagnostic tool in resource-limited settings where access to histopathology is delayed. Early molecular identification of high-risk HPV may support timely clinical decision-making and facilitate linkage to care. However, its clinical impact remains constrained in the context of advanced disease at presentation, underscoring the importance of addressing structural barriers to early diagnosis and sustained HIV and cancer care in underserved regions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s40249-026-01457-2","type":"journal-article","created":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T02:10:36Z","timestamp":1780279836000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Repurposing GeneXpert\u00ae for rapid diagnosis of HPV-associated anal cancer: a case report of an unusually large lesion"],"prefix":"10.1186","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-5297-225X","authenticated-orcid":false,"given":"Livia","family":"Bresciani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline","family":"Concha-Jaimes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alonso","family":"D\u00edaz-Sheen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge Miguel","family":"Sabina-Vela","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heidy","family":"Sanchez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Rojas-Blasquez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristopher","family":"Pinto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Vassalini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cesar Johnny","family":"Ramal-Asayag","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,6,1]]},"reference":[{"key":"1457_CR1","doi-asserted-by":"publisher","first-page":"795","DOI":"10.1097\/QAD.0000000000001765","volume":"32","author":"G Liu","year":"2018","unstructured":"Liu G, Sharma M, Tan N, et al. 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Documentation of consent is available upon request.Photographic documentation of the lesion was obtained to support clinical assessment and education. All images were fully anonymized, excluding any identifiable features of the patient, in compliance with publication standards.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Written informed consent for the publication of this case report and associated clinical images was obtained from the patient. A signed copy of the consent form is available and can be provided upon request to the journal or relevant authorities.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publications"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"63"}}