{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T23:20:50Z","timestamp":1793748050682,"version":"4.6.1"},"reference-count":33,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,5,9]],"date-time":"2021-05-09T00:00:00Z","timestamp":1620518400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,5,9]],"date-time":"2021-05-09T00:00:00Z","timestamp":1620518400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Cell Pediatr"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Ca<jats:sup>2+<\/jats:sup> as a universal second messenger regulates basic biological functions including cell cycle, cell proliferation, cell differentiation, and cell death. Lack of the protein mitochondrial calcium uptake1 (MICU1), which has been regarded as a gatekeeper of Ca ions, leads to the abnormal mitochondrial Ca<jats:sup>2+<\/jats:sup> handling, excessive production of reactive oxygen species (ROS), and increased cell death. Mutations in <jats:italic>MICU1<\/jats:italic> gene causes a very rare neuromuscular disease, myopathy with extrapyramidal signs (MPXPS), due to primary alterations in mitochondrial calcium signaling which demonstrates the key role of mitochondrial Ca<jats:sup>2+<\/jats:sup> uptake. To date, 13 variants have been reported in <jats:italic>MICU1<\/jats:italic> gene in 44 patients presented with the vast spectrum of symptoms.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Case presentation<\/jats:title>\n                <jats:p>Here, we report a 44-year-old Iranian patient presented with learning disability, muscle weakness, easy fatigability, reduced tendon reflexes, ataxia, gait disturbance, elevated hepatic transaminases, elevated serum creatine kinase (CK), and elevated lactate dehydrogenase (LDH). We identified a novel nonsense variant c.385C&gt;T; p.(R129*) in <jats:italic>MICU1<\/jats:italic> gene by whole exome sequencing (WES) and segregation analysis.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Our finding along with previous studies provides more evidence on the clinical presentation of the disease caused by pathogenic mutations in <jats:italic>MICU1<\/jats:italic>. Finding more variants and expanding the spectrum of the disease increases the diagnostic rate of molecular testing in screening of this kind of diseases and in turn improves the quality of counseling for at risk couples and helps them to minimize the risks of having affected children.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s40348-021-00116-w","type":"journal-article","created":{"date-parts":[[2021,5,9]],"date-time":"2021-05-09T17:02:28Z","timestamp":1620579748000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Identification of a novel MICU1 nonsense variant causes myopathy with extrapyramidal signs in an Iranian consanguineous family"],"prefix":"10.1186","volume":"8","author":[{"given":"Fatemeh","family":"Bitarafan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehrnoosh","family":"Khodaeian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elham","family":"Amjadi Sardehaei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatemeh Zahra","family":"Darvishi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Navid","family":"Almadani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yalda","family":"Nilipour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5508-7903","authenticated-orcid":false,"given":"Masoud","family":"Garshasbi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,5,9]]},"reference":[{"issue":"2","key":"116_CR1","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1038\/ng.2851","volume":"46","author":"CV Logan","year":"2014","unstructured":"Logan CV, Szabadkai G, Sharpe JA, Parry DA, Torelli S, Childs AM et al (2014) Loss-of-function mutations in MICU1 cause a brain and muscle disorder linked to primary alterations in mitochondrial calcium signaling. Nat Genet. 46(2):188\u2013193 Available from: https:\/\/doi.org\/10.1038\/ng.2851","journal-title":"Nat Genet."},{"issue":"5","key":"116_CR2","doi-asserted-by":"publisher","first-page":"1274","DOI":"10.1016\/j.celrep.2019.09.063","volume":"29","author":"V Debattisti","year":"2019","unstructured":"Debattisti V, Horn A, Singh R, Seifert EL, Hogarth MW, Mazala DA et al (2019) Dysregulation of mitochondrial Ca2+ uptake and sarcolemma repair underlie muscle weakness and wasting in patients and mice lacking MICU1. Cell Rep 29(5):1274\u20131286.e6. https:\/\/doi.org\/10.1016\/j.celrep.2019.09.063","journal-title":"Cell Rep"},{"issue":"1","key":"116_CR3","doi-asserted-by":"publisher","first-page":"10955","DOI":"10.1038\/ncomms10955","volume":"7","author":"AN Antony","year":"2016","unstructured":"Antony AN, Paillard M, Moffat C, Juskeviciute E, Correnti J, Bolon B, Rubin E, Csord\u00e1s G, Seifert EL, Hoek JB, Hajn\u00f3czky G (2016) MICU1 regulation of mitochondrial Ca2+ uptake dictates survival and tissue regeneration. Nat Commun. 7(1):10955. Available from: http:\/\/www.nature.com\/articles\/ncomms10955. https:\/\/doi.org\/10.1038\/ncomms10955","journal-title":"Nat Commun."},{"issue":"5","key":"116_CR4","doi-asserted-by":"publisher","first-page":"1541","DOI":"10.1016\/j.celrep.2019.04.033","volume":"27","author":"R Tufi","year":"2019","unstructured":"Tufi R, Gleeson TP, von Stockum S, Hewitt VL, Lee JJ, Terriente-Felix A et al (2019) Comprehensive genetic characterization of mitochondrial Ca 2+ uniporter components reveals their different physiological requirements in vivo. Cell Rep 27(5):1541\u20131550.e5. https:\/\/doi.org\/10.1016\/j.celrep.2019.04.033","journal-title":"Cell Rep"},{"issue":"16","key":"116_CR5","doi-asserted-by":"publisher","first-page":"3447","DOI":"10.1113\/JP270254","volume":"593","author":"I Llorente-Folch","year":"2015","unstructured":"Llorente-Folch I, Rueda CB, Pardo B, Szabadkai G, Duchen MR, Satrustegui J (2015) The regulation of neuronal mitochondrial metabolism by calcium. J Physiol. 593(16):3447\u20133462. https:\/\/doi.org\/10.1113\/JP270254","journal-title":"J Physiol."},{"issue":"1","key":"116_CR6","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1756-6606-5-2","volume":"5","author":"RD Burgoyne","year":"2012","unstructured":"Burgoyne RD, Haynes LP (2012) Understanding the physiological roles of the neuronal calcium sensor proteins. Mol Brain. 5(1):1\u201311. https:\/\/doi.org\/10.1186\/1756-6606-5-2","journal-title":"Mol Brain."},{"key":"116_CR7","doi-asserted-by":"publisher","unstructured":"Kawamoto EM, Vivar C, Camandola S. Physiology and pathology of calcium signaling in the brain. Front Pharmacol. 2012;3 APR(April):1\u201317. doi: https:\/\/doi.org\/10.3389\/fphar.2012.00061","DOI":"10.3389\/fphar.2012.00061"},{"issue":"4","key":"116_CR8","doi-asserted-by":"publisher","first-page":"818","DOI":"10.1371\/journal.pbio.0050066","volume":"5","author":"S Ryglewski","year":"2007","unstructured":"Ryglewski S, Pflueger HJ, Duch C (2007) Expanding the neuron\u2019s calcium signaling repertoire: Intracellular calcium release via voltage-induced PLC and IP3R activation. PLoS Biol. 5(4):818\u2013827. https:\/\/doi.org\/10.1371\/journal.pbio.0050066","journal-title":"PLoS Biol."},{"issue":"24","key":"116_CR9","doi-asserted-by":"publisher","first-page":"17610","DOI":"10.1074\/jbc.M112.435206","volume":"288","author":"RR Arvizo","year":"2013","unstructured":"Arvizo RR, Moyano DF, Saha S, Thompson MA, Bhattacharya R, Rotello VM, Prakash YS, Mukherjee P (2013) Probing novel roles of the mitochondrial uniporter in ovarian cancer cells using nanoparticles. J Biol Chem. 288(24):17610\u201317618. https:\/\/doi.org\/10.1074\/jbc.M112.435206","journal-title":"J Biol Chem."},{"issue":"2","key":"116_CR10","doi-asserted-by":"publisher","first-page":"4","DOI":"10.7554\/eLife.00704","volume":"2013","author":"D Chaudhuri","year":"2013","unstructured":"Chaudhuri D, Sancak Y, Mootha VK, Clapham DE (2013) MCU encodes the pore conducting mitochondrial calcium currents. Elife. 2013(2):4\u201311. https:\/\/doi.org\/10.7554\/eLife.00704","journal-title":"Elife."},{"issue":"3","key":"116_CR11","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1016\/j.celrep.2015.12.054","volume":"14","author":"H Vais","year":"2016","unstructured":"Vais H, Mallilankaraman K, Mak DOD, Hoff H, Payne R, Tanis JE, Foskett JK (2016) EMRE is a matrix Ca2+ sensor that governs gatekeeping of the mitochondrial Ca2+ uniporter. Cell Rep. 14(3):403\u2013410 Available from: https:\/\/doi.org\/10.1016\/j.celrep.2015.12.054","journal-title":"Cell Rep."},{"issue":"5","key":"116_CR12","doi-asserted-by":"publisher","first-page":"1364","DOI":"10.1016\/j.celrep.2019.04.013","volume":"27","author":"D Flicker","year":"2019","unstructured":"Flicker D, Sancak Y, Mick E, Goldberger O, Mootha VK (2019) Exploring the in vivo role of the mitochondrial calcium uniporter in brown fat bioenergetics. Cell Rep 27(5):1364\u20131375.e5. https:\/\/doi.org\/10.1016\/j.celrep.2019.04.013","journal-title":"Cell Rep"},{"issue":"1","key":"116_CR13","doi-asserted-by":"publisher","first-page":"12897","DOI":"10.1038\/ncomms12897","volume":"7","author":"CT Madreiter-Sokolowski","year":"2016","unstructured":"Madreiter-Sokolowski CT, Klec C, Parichatikanond W, Stryeck S, Gottschalk B, Pulido S, Rost R, Eroglu E, Hofmann NA, Bondarenko AI, Madl T, Waldeck-Weiermair M, Malli R, Graier WF (2016) PRMT1-mediated methylation of MICU1 determines the UCP2\/3 dependency of mitochondrial Ca2+ uptake in immortalized cells. Nat Commun. 7(1):12897 Available from: https:\/\/doi.org\/10.1038\/ncomms12897","journal-title":"Nat Commun."},{"issue":"6","key":"116_CR14","doi-asserted-by":"publisher","first-page":"976","DOI":"10.1016\/j.cmet.2013.04.020","volume":"17","author":"G Csord\u00e1s","year":"2013","unstructured":"Csord\u00e1s G, Golen\u00e1r T, Seifert EL, Kamer KJ, Sancak Y, Perocchi F, Moffat C, Weaver D, Perez SF, Bogorad R, Koteliansky V, Adijanto J, Mootha VK, Hajn\u00f3czky G (2013) MICU1 controls both the threshold and cooperative activation of the mitochondrial Ca2+ uniporter. Cell Metab. 17(6):976\u2013987. https:\/\/doi.org\/10.1016\/j.cmet.2013.04.020","journal-title":"Cell Metab."},{"issue":"9","key":"116_CR15","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1038\/nrm4039","volume":"16","author":"KJ Kamer","year":"2015","unstructured":"Kamer KJ, Mootha VK (2015) The molecular era of the mitochondrial calcium uniporter. Nat Rev Mol Cell Biol. 16(9):545\u2013553 Available from: https:\/\/doi.org\/10.1038\/nrm4039","journal-title":"Nat Rev Mol Cell Biol."},{"issue":"6","key":"116_CR16","doi-asserted-by":"publisher","first-page":"1561","DOI":"10.1016\/j.celrep.2016.07.011","volume":"16","author":"JC Liu","year":"2016","unstructured":"Liu JC, Liu J, Holmstr\u00f6m KM, Menazza S, Parks RJ, Fergusson MM, Yu ZX, Springer DA, Halsey C, Liu C, Murphy E, Finkel T (2016) MICU1 serves as a molecular gatekeeper to prevent in vivo mitochondrial calcium overload. Cell Rep. 16(6):1561\u20131573. https:\/\/doi.org\/10.1016\/j.celrep.2016.07.011","journal-title":"Cell Rep."},{"issue":"1\u20132","key":"116_CR17","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1007\/s11010-015-2554-4","volume":"410","author":"T Furuno","year":"2015","unstructured":"Furuno T, Shinkai N, Inoh Y, Nakanishi M (2015) Impaired expression of the mitochondrial calcium uniporter suppresses mast cell degranulation. Mol Cell Biochem. 410(1\u20132):215\u2013221. https:\/\/doi.org\/10.1007\/s11010-015-2554-4","journal-title":"Mol Cell Biochem."},{"issue":"6","key":"116_CR18","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1002\/embj.201386523","volume":"33","author":"L Wang","year":"2014","unstructured":"Wang L, Yang X, Li S, Wang Z, Liu Y, Feng J, Zhu Y, Shen Y (2014) Structural and mechanistic insights into MICU1 regulation of mitochondrial calcium uptake. EMBO J. 33(6):594\u2013604 Available from: http:\/\/emboj.embopress.org\/cgi\/doi\/10.1002\/embj.201386523","journal-title":"EMBO J."},{"issue":"6","key":"116_CR19","doi-asserted-by":"publisher","first-page":"1009","DOI":"10.1016\/j.bbamcr.2017.01.015","volume":"1864","author":"G Bhosale","year":"2017","unstructured":"Bhosale G, Sharpe JA, Koh A, Kouli A, Szabadkai G, Duchen MR (2017) Pathological consequences of MICU1 mutations on mitochondrial calcium signalling and bioenergetics. Biochim Biophys Acta - Mol Cell Res. 1864(6):1009\u20131017. https:\/\/doi.org\/10.1016\/j.bbamcr.2017.01.015","journal-title":"Biochim Biophys Acta - Mol Cell Res."},{"issue":"1","key":"116_CR20","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1093\/nar\/gkg129","volume":"31","author":"D Karolchik","year":"2003","unstructured":"Karolchik D, Baertsch R, Diekhans M, Furey TS, Hinrichs A, Lu YT, Roskin KM, Schwartz M, Sugnet CW, Thomas DJ, Weber RJ, Haussler D, Kent WJ, University of California Santa Cruz (2003) The UCSC Genome Browser Database. Nucleic Acids Res. 31(1):51\u201354. https:\/\/doi.org\/10.1093\/nar\/gkg129","journal-title":"Nucleic Acids Res."},{"issue":"5","key":"116_CR21","doi-asserted-by":"publisher","first-page":"405","DOI":"10.1038\/gim.2015.30","volume":"17","author":"S Richards","year":"2015","unstructured":"Richards S, Aziz N, Bale S, Bick D, Das S, Gastier-Foster J et al (2015) Standards and guidelines for the interpretation of sequence variants: a joint consensus recommendation of the American College of Medical Genetics and Genomics and the Association for Molecular Pathology. Genet Med. 17(5):405\u2013424. https:\/\/doi.org\/10.1038\/gim.2015.30","journal-title":"Genet Med."},{"issue":"20","key":"116_CR22","doi-asserted-by":"publisher","first-page":"3359","DOI":"10.1093\/bioinformatics\/btv362","volume":"31","author":"W Liu","year":"2015","unstructured":"Liu W, Xie Y, Ma J, Luo X, Nie P, Zuo Z, Lahrmann U, Zhao Q, Zheng Y, Zhao Y, Xue Y, Ren J (2015) IBS: An illustrator for the presentation and visualization of biological sequences. Bioinformatics. 31(20):3359\u20133361. https:\/\/doi.org\/10.1093\/bioinformatics\/btv362","journal-title":"Bioinformatics."},{"issue":"3","key":"116_CR23","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1136\/jmg.33.3.212","volume":"33","author":"E Hoodfar","year":"1996","unstructured":"Hoodfar E, Teebi AS (1996) Genetic referrals of Middle Eastern origin in a western city: inbreeding and disease profile. J Med Genet. 33(3):212\u2013215. https:\/\/doi.org\/10.1136\/jmg.33.3.212","journal-title":"J Med Genet."},{"issue":"2","key":"116_CR24","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1212\/NXG.0000000000000059","volume":"2","author":"D Lewis-Smith","year":"2016","unstructured":"Lewis-Smith D, Kamer KJ, Griffin H, Childs AM, Pysden K, Titov D, Duff J, Pyle A, Taylor RW, Yu-Wai-Man P, Ramesh V, Horvath R, Mootha VK, Chinnery PF (2016) Homozygous deletion in MICU1 presenting with fatigue and lethargy in childhood. Neurol Genet. 2(2):1\u20136. https:\/\/doi.org\/10.1212\/NXG.0000000000000059","journal-title":"Neurol Genet."},{"issue":"6","key":"116_CR25","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1002\/ajmg.a.61126","volume":"179","author":"N Al-Dewik","year":"2019","unstructured":"Al-Dewik N, Mohd H, Al-Mureikhi M, Ali R, Al-Mesaifri F, Mahmoud L et al (2019) Clinical exome sequencing in 509 Middle Eastern families with suspected Mendelian diseases: the Qatari experience. Am J Med Genet Part A. 179(6):927\u2013935 Available from: https:\/\/onlinelibrary.wiley.com\/doi\/abs\/10.1002\/ajmg.a.61126","journal-title":"Am J Med Genet Part A."},{"key":"116_CR26","doi-asserted-by":"publisher","unstructured":"Alfares A, Alfadhel M, Wani T, Alsahli S, Alluhaydan I, Al F et al (2017) A multicenter clinical exome study in unselected cohorts from a consanguineous population of Saudi Arabia demonstrated a high diagnostic yield. Mol Genet Metab. https:\/\/doi.org\/10.1016\/j.ymgme.2017.04.002","DOI":"10.1016\/j.ymgme.2017.04.002"},{"key":"116_CR27","doi-asserted-by":"publisher","DOI":"10.1007\/8904","volume-title":"Research report a Middle Eastern Founder mutation expands the genotypic and phenotypic spectrum of mitochondrial MICU1 deficiency\u00a0: a report of 13 patients","author":"S Musa","year":"2018","unstructured":"Musa S, Eyaid W, Kamer K (2018) Research report a Middle Eastern Founder mutation expands the genotypic and phenotypic spectrum of mitochondrial MICU1 deficiency\u00a0: a report of 13 patients. https:\/\/doi.org\/10.1007\/8904"},{"issue":"1","key":"116_CR28","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1002\/ana.24687","volume":"80","author":"GL O\u2019Grady","year":"2016","unstructured":"O\u2019Grady GL, Lek M, Lamande SR, Waddell L, Oates EC, Punetha J et al (2016) Diagnosis and etiology of congenital muscular dystrophy: we are halfway there. Ann Neurol. 80(1):101\u2013111. https:\/\/doi.org\/10.1002\/ana.24687","journal-title":"Ann Neurol."},{"key":"116_CR29","doi-asserted-by":"publisher","unstructured":"Wilton KM, Morales-rosado JA, Selcen D, Klee EW, Morava MHE (2020) Developmental brain abnormalities and acute encephalopathy in a patient with myopathy with extrapyramidal signs secondary to pathogenic variants in MICU1. (February):22\u201328. https:\/\/doi.org\/10.1002\/jmd2.12114","DOI":"10.1002\/jmd2.12114"},{"issue":"2017","key":"116_CR30","doi-asserted-by":"publisher","first-page":"S117","DOI":"10.1016\/j.nmd.2017.06.094","volume":"27","author":"A Roos","year":"2017","unstructured":"Roos A, Topf A, Phan V, Gonzalez Coraspe J, Hauessler M, Weis J et al (2017) Insights into the genesis of a brain and muscle disorder caused by a novel mutation in MICU1. Neuromuscul Disord 27(2017):S117. https:\/\/doi.org\/10.1016\/j.nmd.2017.06.094","journal-title":"Neuromuscul Disord"},{"issue":"3","key":"116_CR31","doi-asserted-by":"publisher","first-page":"567","DOI":"10.1111\/cge.13102","volume":"93","author":"E Ch\u00e9rot","year":"2018","unstructured":"Ch\u00e9rot E, Keren B, Dubourg C, Carr\u00e9 W, Fradin M, Lavillaureix A, Afenjar A, Burglen L, Whalen S, Charles P, Marey I, Heide S, Jacquette A, Heron D, Doummar D, Rodriguez D, Billette de Villemeur T, Moutard ML, Gu\u00ebt A, Xavier J, P\u00e9risse D, Cohen D, Demurger F, Qu\u00e9lin C, Depienne C, Odent S, Nava C, David V, Pasquier L, Mignot C (2018) Using medical exome sequencing to identify the causes of neurodevelopmental disorders: experience of 2 clinical units and 216 patients. Clin Genet. 93(3):567\u2013576. Available from:. https:\/\/doi.org\/10.1111\/cge.13102","journal-title":"Clin Genet."},{"issue":"1","key":"116_CR32","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1186\/s12881-020-01016-y","volume":"21","author":"M Mojbafan","year":"2020","unstructured":"Mojbafan M, Nojehdeh ST, Rahiminejad F, Nilipour Y, Tonekaboni SH, Zeinali S (2020) Reporting a rare form of myopathy, myopathy with extrapyramidal signs, in an Iranian family using next generation sequencing: a case report. BMC Med Genet. 21(1):4\u20139. https:\/\/doi.org\/10.1186\/s12881-020-01016-y","journal-title":"BMC Med Genet."},{"issue":"2","key":"116_CR33","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1080\/03014460310001652211","volume":"31","author":"M Saadat","year":"2004","unstructured":"Saadat M, Ansari-Lari M, Farhud DD (2004) Consanguineous marriage in Iran. Ann Hum Biol. 31(2):263\u2013269. https:\/\/doi.org\/10.1080\/03014460310001652211","journal-title":"Ann Hum Biol."}],"container-title":["Molecular and Cellular Pediatrics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s40348-021-00116-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s40348-021-00116-w\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s40348-021-00116-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,5,9]],"date-time":"2021-05-09T17:02:32Z","timestamp":1620579752000},"score":1,"resource":{"primary":{"URL":"https:\/\/molcellped.springeropen.com\/articles\/10.1186\/s40348-021-00116-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,9]]},"references-count":33,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["116"],"URL":"https:\/\/doi.org\/10.1186\/s40348-021-00116-w","relation":{},"ISSN":["2194-7791"],"issn-type":[{"value":"2194-7791","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,9]]},"assertion":[{"value":"3 January 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 April 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 May 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have conducted the project ethically in accordance with the World Medical Association Declaration of Helsinki. The written, informed consent was obtained from all participants or their respective guardians. Participants also provided the written informed consent for publication of their related information included in this paper.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Written informed consent was provided for publication of all the available participants included in this paper.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"6"}}