{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T14:20:24Z","timestamp":1765808424283,"version":"3.32.0"},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,12,21]],"date-time":"2024-12-21T00:00:00Z","timestamp":1734739200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,12,21]],"date-time":"2024-12-21T00:00:00Z","timestamp":1734739200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Cell Pediatr"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>In neonates with congenital heart disease (CHD), myocardial remodelling involves activation of inflammatory pathways. The role of hypoxemia related pathways is however unknown. This study was therefore designed to investigate myocardial mRNA expression of interleukin (IL)-6 and hypoxia-inducible factor (HIF)-1\u03b1 in neonates with CHD and analyse its influence on post-operative outcome.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>14 neonates with CHD scheduled for open cardiac surgery were studied. In group 1 (<jats:italic>n<\/jats:italic>\u2009=\u20095), pre-operative transcutaneous arterial oxygen saturation (SaO<jats:sub>2<\/jats:sub>) was \u2264\u200985% and in group 2 (<jats:italic>n<\/jats:italic>\u2009=\u20099)\u2009&gt;\u200985%. Expression of IL-6- and HIF-1\u03b1-mRNA was studied on right atrial biopsy by RT-PCR and corelated to post-operative (po) outcome. Group 1 patients showed higher mean arterial blood pressure (MAP) and lower glycaemia 4\u00a0h po (<jats:italic>p<\/jats:italic>\u2009=\u20090.047 and <jats:italic>p<\/jats:italic>\u2009=\u20090.021, respectively). In the whole cohort, SaO<jats:sub>2<\/jats:sub> correlated negatively with MAP (Pearson R: -0.662, <jats:italic>p<\/jats:italic>\u2009=\u20090.010). mRNA coding for IL-6 and HIF-1\u03b1 was detected in the myocardium of all neonates independently of age, gender, or type of CHD. IL6-mRNA expression was not influenced by pre-operative hypoxemia but was associated with higher lactate levels in early po period (Pearson R: 0,611, <jats:italic>p<\/jats:italic>\u2009=\u20090,020). HIF-1\u03b1-mRNA expression correlated negatively with pre-operative SaO<jats:sub>2<\/jats:sub> (Pearson R: -0.551, <jats:italic>p<\/jats:italic>\u2009=\u20090.04) and with aspartate aminotransferase levels 4\u00a0h po (Pearson R: 0.625, <jats:italic>p<\/jats:italic>\u2009=\u20090.017).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>Our study shows that besides inflammatory pathways, hypoxemia related pathways are activated in the myocardium of neonates with CHD. Myocardial expression of both IL-6-mRNA and HIF-1\u03b1-mRNA relates to biological markers of a worse po outcome.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s40348-024-00187-5","type":"journal-article","created":{"date-parts":[[2024,12,21]],"date-time":"2024-12-21T04:43:40Z","timestamp":1734756220000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Myocardial mRNA expression of interleukin-6 and hypoxia inducible factor-1\u03b1 in neonates with congenital cardiac defects"],"prefix":"10.1186","volume":"11","author":[{"given":"Nesrine","family":"Farhat","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaime","family":"Vazquez-Jimenez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruth","family":"Heying","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie-Christine","family":"Seghaye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,12,21]]},"reference":[{"issue":"2","key":"187_CR1","doi-asserted-by":"publisher","first-page":"601","DOI":"10.1016\/S0003-4975(01)03391-4","volume":"73","author":"HH H\u00f6vels-G\u00fcrich","year":"2002","unstructured":"H\u00f6vels-G\u00fcrich HH, Schumacher K, Vazquez-Jimenez JF, Qing M, H\u00fcffmeier U, Buding B, Messmer BJ, von Bernuth G, Seghaye MC (2002) Cytokine balance in infants undergoing cardiac operation. 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