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The binding of the viral Spike glycoprotein to the human cell receptor angiotensin-converting enzyme 2 (ACE2) initiates viral infection. The development of efficient treatments to combat coronavirus disease is considered essential.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>An in silico approach was employed to design amino acid peptide inhibitor against the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein. The designed inhibitor (SARS-CoV-2 PEP 49) consists of amino acids with the \u03b11 helix and the \u03b24 - \u03b25 sheets of ACE2. The PEP-FOLD3 web tool was used to create the 3D structures of the peptide amino acids. Analyzing the interaction between ACE2 and the RBD of the Spike protein for three protein data bank entries (6M0J, 7C8D, and 7A95) indicated that the interacting amino acids were contained inside two regions of ACE2: the \u03b11 helical protease domain (PD) and the \u03b24 - \u03b25 sheets.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Molecular docking analysis of the designed inhibitor demonstrated that SARS-CoV-2 PEP 49 attaches directly to the ACE2 binding site of the Spike protein with a binding affinity greater than the ACE2, implying that the SARS-CoV-2 PEP 49 model may be useful as a potential RBD binding blocker.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s40360-022-00627-w","type":"journal-article","created":{"date-parts":[[2022,12,2]],"date-time":"2022-12-02T08:34:11Z","timestamp":1669970051000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Potential antiviral peptides targeting the SARS-CoV-2 spike protein"],"prefix":"10.1186","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5722-7906","authenticated-orcid":false,"given":"Ibrahim","family":"Khater","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5542-9387","authenticated-orcid":false,"given":"Aaya","family":"Nassar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,12,2]]},"reference":[{"key":"627_CR1","volume-title":"Features, evaluation and treatment coronavirus (COVID-19), StatPearls","author":"M Cascella","year":"2020","unstructured":"Cascella M, Rajnik M, Cuomo A, Dulebohn SC, Di Napoli R. 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