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We demonstrate that soluble A\u03b2 accumulation is present in the retina of these mice early in life and progresses to A\u03b2 plaque formation by midlife. This rising A\u03b2 burden coincides with local microglia reactivity, astrogliosis, and abnormalities in retinal vein morphology. Electrophysiological recordings revealed signs of neuronal dysfunction yet no overt neurodegeneration was observed and visual performance outcomes were unaffected in the\n                    <jats:italic>App<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>NL<\/jats:italic>\n                      -\n                      <jats:italic>G<\/jats:italic>\n                      -\n                      <jats:italic>F<\/jats:italic>\n                    <\/jats:sup>\n                    mouse. Furthermore, we show that hyperspectral imaging can be used to quantify retinal A\u03b2, underscoring its potential as a biomarker for AD diagnosis and monitoring. 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