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AD is characterised by the formation of plaques containing extracellular amyloid-\u03b2 (A\u03b2) and neurofibrillary tangles composed of hyper-phosphorylated tau proteins (pTau). A\u03b2 gradually accumulates in the brain up to 20\u00a0years before the clinical onset of dementia, making it a compelling candidate for early detection of AD. It has been shown that there is increased deposition of A\u03b2s in AD patients\u2019 retinas. However, little is known about microglia\u2019s ability to function and clear A\u03b2 within the retina of AD and control eyes. We labelled microglia with ionised calcium-binding adaptor molecule 1 (IBA-1) in AD and age-matched control donor retinas. We then used interactive machine learning to segment individual microglia in 3D. In the temporal mid-peripheral region, we found that the number of microglia was significantly lower in AD retinas compared to controls. Unexpectedly, the size of the microglia was significantly larger in the AD retinas compared to controls. We also labelled retinal microglia for Cluster of Differentiation 68 (CD68), a transmembrane glycoprotein expressed by cells in the monocyte lineage and a marker of phagocytic activity and activated microglia. The size of CD68\u2009+\u2009cells was statistically different between AD and control microglial, with CD68\u2009+\u2009cells being larger in AD. In contrast, there was no difference in either size or shape for CD68- microglia between the two groups, suggesting an important difference in the active states of CD68\u2009+\u2009microglia in AD retina. There was also significantly increased CD68 immunoreactivity in individual microglia within the AD group. 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