{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T07:23:49Z","timestamp":1793949829756,"version":"4.8.0"},"reference-count":43,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T00:00:00Z","timestamp":1734307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T00:00:00Z","timestamp":1734307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Science research cultivation program of stomatological hospital, Southern medical university","award":["PY2023038"],"award-info":[{"award-number":["PY2023038"]}]},{"name":"Science research cultivation program of stomatological hospital, Southern medical university","award":["PY2023043"],"award-info":[{"award-number":["PY2023043"]}]},{"name":"Guangdong Medical Research Fund","award":["B2023274"],"award-info":[{"award-number":["B2023274"]}]},{"name":"Guangdong Medical Research Fund","award":["B2022013"],"award-info":[{"award-number":["B2022013"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Prog Orthod."],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>White spot lesions (WSLs) may develop in adolescents undergoing clear aligner (CA) therapy with poor oral hygiene. The specific effects of CAs on the microbial composition and functional characteristics of supragingival plaques remain unclear. The present study investigated the shift in the supragingival microbial community induced by CAs in adolescents through metagenomic technology.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Fifteen adolescents (12\u201315 years old) with Invisalign appliances were recruited. Supragingival plaque specimens were obtained twice, before treatment (T\n                      <jats:sub>1<\/jats:sub>\n                      ) and three months after treatment (T\n                      <jats:sub>2<\/jats:sub>\n                      ). All the bacterial plaque specimens were analyzed for microbial communities and functions using metagenomic analyses.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 2,840,242,722 reads disclosed 180 phyla, 3,975 genera, and 16,497 microbiome species. During the first three months, the microbial community was relatively stable. The genus level revealed a higher relative abundance of\n                      <jats:italic>Capnocytophaga<\/jats:italic>\n                      ,\n                      <jats:italic>Neisseria<\/jats:italic>\n                      , and\n                      <jats:italic>Arachnia<\/jats:italic>\n                      in the T\n                      <jats:sub>2<\/jats:sub>\n                      period. Furthermore, the functional analysis suggested that the relative abundances of folate biosynthesis, biotin metabolism and biofilm formation-vibrio cholerae were increased in the T\n                      <jats:sub>2<\/jats:sub>\n                      period compared to the T\n                      <jats:sub>1<\/jats:sub>\n                      period. Finally, virulence factor analysis demonstrated that the relative abundance of genes associated with type IV pili (VF0082) and polar flagella (VF0473) was higher in the T\n                      <jats:sub>2<\/jats:sub>\n                      period than in the T\n                      <jats:sub>1<\/jats:sub>\n                      period.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>In adolescents undergoing CA therapy with poor plaque control, caries progresses quickly within three months and noticeable WSLs develop on the tooth surface. Although the microbial community remained relatively steady and CA therapy did not cause significant changes in the overall functional gene composition in the first three months, virulence factors, including type IV pili and flagella, were more abundant and actively contributed to microorganism adhesion and biofilm formation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s40510-024-00547-x","type":"journal-article","created":{"date-parts":[[2024,12,15]],"date-time":"2024-12-15T19:01:58Z","timestamp":1734289318000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["The microbiome alterations of supragingival plaque among adolescents using clear aligners: a metagenomic sequencing analysis"],"prefix":"10.1186","volume":"25","author":[{"given":"Chunlin","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuyu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6389-8360","authenticated-orcid":false,"given":"Hai","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,12,16]]},"reference":[{"issue":"2","key":"547_CR1","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1016\/j.ajodo.2017.10.009","volume":"153","author":"A Chhibber","year":"2018","unstructured":"Chhibber A, Agarwal S, Yadav S, Kuo CL, Upadhyay M. Which orthodontic appliance is best for oral hygiene? A randomized clinical trial. Am J Orthod Dentofac Orthop. 2018;153(2):175\u201383.","journal-title":"Am J Orthod Dentofac Orthop"},{"issue":"5","key":"547_CR2","doi-asserted-by":"publisher","first-page":"594","DOI":"10.1016\/j.ajodo.2020.01.004","volume":"157","author":"Z Albhaisi","year":"2020","unstructured":"Albhaisi Z, Al-Khateeb SN, Abu Alhaija ES. Enamel demineralization during clear aligner orthodontic treatment compared with fixed appliance therapy, evaluated with quantitative light-induced fluorescence: a randomized clinical trial. Am J Orthod Dentofac Orthop. 2020;157(5):594\u2013601.","journal-title":"Am J Orthod Dentofac Orthop"},{"issue":"5","key":"547_CR3","doi-asserted-by":"publisher","first-page":"603","DOI":"10.1016\/j.identj.2023.03.012","volume":"73","author":"M Rouzi","year":"2023","unstructured":"Rouzi M, Zhang X, Jiang Q, Long H, Lai W, Li X. Impact of clear aligners on oral health and oral Microbiome during Orthodontic Treatment. Int Dent J. 2023;73(5):603\u201311.","journal-title":"Int Dent J"},{"key":"547_CR4","doi-asserted-by":"crossref","unstructured":"Liu Q, Song Z. Incidence, severity, and risk factors for white spot lesions in adolescent patients treated with clear aligners. Orthod Craniofac Res. 2024.","DOI":"10.1111\/ocr.12791"},{"issue":"1","key":"547_CR5","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1016\/0889-5406(88)90453-2","volume":"94","author":"B Ogaard","year":"1988","unstructured":"Ogaard B, R\u00f8lla G, Arends J. Orthodontic appliances and enamel demineralization. Part 1. Lesion development. Am J Orthod Dentofac Orthop. 1988;94(1):68\u201373.","journal-title":"Am J Orthod Dentofac Orthop"},{"key":"547_CR6","doi-asserted-by":"publisher","first-page":"1119616","DOI":"10.3389\/fcimb.2023.1119616","volume":"13","author":"Z Song","year":"2023","unstructured":"Song Z, Fang S, Guo T, Wen Y, Liu Q, Jin Z. Microbiome and metabolome associated with white spot lesions in patients treated with clear aligners. Front Cell Infect Microbiol. 2023;13:1119616.","journal-title":"Front Cell Infect Microbiol"},{"issue":"1","key":"547_CR7","doi-asserted-by":"publisher","first-page":"268","DOI":"10.1186\/s12864-019-5641-1","volume":"20","author":"JL Sevigny","year":"2019","unstructured":"Sevigny JL, Rothenheber D, Diaz KS, Zhang Y, Agustsson K, Bergeron RD, et al. Marker genes as predictors of shared genomic function. BMC Genomics. 2019;20(1):268.","journal-title":"BMC Genomics"},{"issue":"5","key":"547_CR8","doi-asserted-by":"publisher","first-page":"e475","DOI":"10.1016\/j.ajodo.2021.10.015","volume":"161","author":"B Shokeen","year":"2022","unstructured":"Shokeen B, Viloria E, Duong E, Rizvi M, Murillo G, Mullen J, et al. The impact of fixed orthodontic appliances and clear aligners on the oral microbiome and the association with clinical parameters: a longitudinal comparative study. Am J Orthod Dentofac Orthop. 2022;161(5):e475\u201385.","journal-title":"Am J Orthod Dentofac Orthop"},{"issue":"1","key":"547_CR9","doi-asserted-by":"publisher","first-page":"5029","DOI":"10.1038\/s41467-019-13036-1","volume":"10","author":"JS Johnson","year":"2019","unstructured":"Johnson JS, Spakowicz DJ, Hong BY, Petersen LM, Demkowicz P, Chen L, et al. Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis. Nat Commun. 2019;10(1):5029.","journal-title":"Nat Commun"},{"issue":"2","key":"547_CR10","doi-asserted-by":"publisher","first-page":"566","DOI":"10.1007\/s00284-020-02308-5","volume":"78","author":"D Yan","year":"2021","unstructured":"Yan D, Liu Y, Che X, Mi S, Jiao Y, Guo L, et al. Changes in the Microbiome of the Inner Surface of Clear aligners after different usage periods. Curr Microbiol. 2021;78(2):566\u201375.","journal-title":"Curr Microbiol"},{"issue":"2","key":"547_CR11","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1111\/ocr.12427","volume":"24","author":"L Lombardo","year":"2021","unstructured":"Lombardo L, Palone M, Scapoli L, Siciliani G, Carinci F. Short-term variation in the subgingival microbiota in two groups of patients treated with clear aligners and vestibular fixed appliances: a longitudinal study. Orthod Craniofac Res. 2021;24(2):251\u201360.","journal-title":"Orthod Craniofac Res"},{"issue":"2","key":"547_CR12","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1111\/odi.13175","volume":"26","author":"R Zhao","year":"2020","unstructured":"Zhao R, Huang R, Long H, Li Y, Gao M, Lai W. The dynamics of the oral microbiome and oral health among patients receiving clear aligner orthodontic treatment. Oral Dis. 2020;26(2):473\u201383.","journal-title":"Oral Dis"},{"key":"547_CR13","doi-asserted-by":"crossref","unstructured":"Wang Y, Wang S, Wu C, Chen X, Duan Z, Xu Q et al. Oral microbiome alterations Associated with Early Childhood Caries highlight the importance of Carbohydrate metabolic activities. mSystems. 2019;4(6).","DOI":"10.1128\/msystems.00450-19"},{"issue":"1","key":"547_CR14","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1186\/s40168-021-01056-3","volume":"9","author":"K Altabtbaei","year":"2021","unstructured":"Altabtbaei K, Maney P, Ganesan SM, Dabdoub SM, Nagaraja HN, Kumar PS. Anna Karenina and the subgingival microbiome associated with periodontitis. Microbiome. 2021;9(1):97.","journal-title":"Microbiome"},{"key":"547_CR15","doi-asserted-by":"crossref","unstructured":"Purushothaman S, Meola M, Egli A. Combination of whole genome sequencing and Metagenomics for Microbiological Diagnostics. Int J Mol Sci. 2022;23(17).","DOI":"10.3390\/ijms23179834"},{"issue":"11","key":"547_CR16","doi-asserted-by":"publisher","first-page":"292","DOI":"10.1007\/s11274-023-03738-6","volume":"39","author":"N Goel","year":"2023","unstructured":"Goel N, Zaidi S, Khare SK. Whole genome sequencing and functional analysis of a novel biofilm-eradicating strain Nocardiopsis lucentensis EMB25. World J Microbiol Biotechnol. 2023;39(11):292.","journal-title":"World J Microbiol Biotechnol"},{"key":"547_CR17","doi-asserted-by":"publisher","first-page":"361","DOI":"10.3389\/fcimb.2018.00361","volume":"8","author":"Y Zheng","year":"2018","unstructured":"Zheng Y, Zhang M, Li J, Li Y, Teng F, Jiang H, et al. Comparative analysis of the Microbial profiles in Supragingival Plaque samples obtained from twins with discordant caries phenotypes and their mothers. Front Cell Infect Microbiol. 2018;8:361.","journal-title":"Front Cell Infect Microbiol"},{"issue":"6","key":"547_CR18","doi-asserted-by":"publisher","first-page":"Suppl610","DOI":"10.1902\/jop.1967.38.6.610","volume":"38","author":"H L\u00f6e","year":"1967","unstructured":"L\u00f6e H. The Gingival Index, the Plaque Index and the Retention Index systems. J Periodontol. 1967;38(6):Suppl610\u20136.","journal-title":"J Periodontol"},{"issue":"17","key":"547_CR19","doi-asserted-by":"publisher","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","volume":"34","author":"S Chen","year":"2018","unstructured":"Chen S, Zhou Y, Chen Y, Gu J. Fastp: an ultra-fast all-in-one FASTQ preprocessor. Bioinformatics. 2018;34(17):i884\u201390.","journal-title":"Bioinformatics"},{"issue":"14","key":"547_CR20","doi-asserted-by":"publisher","first-page":"1754","DOI":"10.1093\/bioinformatics\/btp324","volume":"25","author":"H Li","year":"2009","unstructured":"Li H, Durbin R. Fast and accurate short read alignment with Burrows-Wheeler transform. Bioinformatics. 2009;25(14):1754\u201360.","journal-title":"Bioinformatics"},{"issue":"10","key":"547_CR21","doi-asserted-by":"publisher","first-page":"1674","DOI":"10.1093\/bioinformatics\/btv033","volume":"31","author":"D Li","year":"2015","unstructured":"Li D, Liu CM, Luo R, Sadakane K, Lam TW. MEGAHIT: an ultra-fast single-node solution for large and complex metagenomics assembly via succinct de bruijn graph. Bioinformatics. 2015;31(10):1674\u20136.","journal-title":"Bioinformatics"},{"key":"547_CR22","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1186\/1471-2105-11-119","volume":"11","author":"D Hyatt","year":"2010","unstructured":"Hyatt D, Chen GL, Locascio PF, Land ML, Larimer FW, Hauser LJ. Prodigal: prokaryotic gene recognition and translation initiation site identification. BMC Bioinformatics. 2010;11:119.","journal-title":"BMC Bioinformatics"},{"issue":"19","key":"547_CR23","doi-asserted-by":"publisher","first-page":"5623","DOI":"10.1093\/nar\/gkl723","volume":"34","author":"H Noguchi","year":"2006","unstructured":"Noguchi H, Park J, Takagi T. MetaGene: prokaryotic gene finding from environmental genome shotgun sequences. Nucleic Acids Res. 2006;34(19):5623\u201330.","journal-title":"Nucleic Acids Res"},{"issue":"23","key":"547_CR24","doi-asserted-by":"publisher","first-page":"3150","DOI":"10.1093\/bioinformatics\/bts565","volume":"28","author":"L Fu","year":"2012","unstructured":"Fu L, Niu B, Zhu Z, Wu S, Li W. CD-HIT: accelerated for clustering the next-generation sequencing data. Bioinformatics. 2012;28(23):3150\u20132.","journal-title":"Bioinformatics"},{"issue":"5","key":"547_CR25","doi-asserted-by":"publisher","first-page":"713","DOI":"10.1093\/bioinformatics\/btn025","volume":"24","author":"R Li","year":"2008","unstructured":"Li R, Li Y, Kristiansen K, Wang J. SOAP: short oligonucleotide alignment program. Bioinformatics. 2008;24(5):713\u20134.","journal-title":"Bioinformatics"},{"issue":"1","key":"547_CR26","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1038\/nmeth.3176","volume":"12","author":"B Buchfink","year":"2015","unstructured":"Buchfink B, Xie C, Huson DH. Fast and sensitive protein alignment using DIAMOND. Nat Methods. 2015;12(1):59\u201360.","journal-title":"Nat Methods"},{"issue":"1","key":"547_CR27","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1186\/s12967-021-02882-7","volume":"19","author":"W Zhou","year":"2021","unstructured":"Zhou W, Zhang D, Li Z, Jiang H, Li J, Ren R, et al. The fecal microbiota of patients with pancreatic ductal adenocarcinoma and autoimmune pancreatitis characterized by metagenomic sequencing. J Transl Med. 2021;19(1):215.","journal-title":"J Transl Med"},{"issue":"5","key":"547_CR28","doi-asserted-by":"publisher","first-page":"598","DOI":"10.1016\/j.ajodo.2011.11.021","volume":"141","author":"C T\u00fcrk\u00f6z","year":"2012","unstructured":"T\u00fcrk\u00f6z C, Canig\u00fcr Bavbek N, Kale Varlik S, Ak\u00e7a G. Influence of thermoplastic retainers on Streptococcus mutans and Lactobacillus adhesion. Am J Orthod Dentofac Orthop. 2012;141(5):598\u2013603.","journal-title":"Am J Orthod Dentofac Orthop"},{"key":"547_CR29","doi-asserted-by":"publisher","first-page":"740981","DOI":"10.3389\/fcimb.2021.740981","volume":"11","author":"L Pang","year":"2021","unstructured":"Pang L, Wang Y, Ye Y, Zhou Y, Zhi Q, Lin H. Metagenomic Analysis of Dental Plaque on pit and Fissure sites with and without caries among adolescents. Front Cell Infect Microbiol. 2021;11:740981.","journal-title":"Front Cell Infect Microbiol"},{"issue":"5","key":"547_CR30","doi-asserted-by":"publisher","first-page":"577","DOI":"10.1093\/ejo\/cjq126","volume":"33","author":"B Low","year":"2011","unstructured":"Low B, Lee W, Seneviratne CJ, Samaranayake LP, H\u00e4gg U. Ultrastructure and morphology of biofilms on thermoplastic orthodontic appliances in \u2018fast\u2019 and \u2018slow\u2019 plaque formers. Eur J Orthod. 2011;33(5):577\u201383.","journal-title":"Eur J Orthod"},{"issue":"4","key":"547_CR31","doi-asserted-by":"publisher","first-page":"e0228798","DOI":"10.1371\/journal.pone.0228798","volume":"15","author":"S Mummolo","year":"2020","unstructured":"Mummolo S, Nota A, Albani F, Marchetti E, Gatto R, Marzo G, et al. Salivary levels of Streptococcus mutans and lactobacilli and other salivary indices in patients wearing clear aligners versus fixed orthodontic appliances: an observational study. PLoS ONE. 2020;15(4):e0228798.","journal-title":"PLoS ONE"},{"key":"547_CR32","doi-asserted-by":"publisher","first-page":"442","DOI":"10.3389\/fcimb.2018.00442","volume":"8","author":"Q Jiang","year":"2018","unstructured":"Jiang Q, Liu J, Chen L, Gan N, Yang D. The oral Microbiome in the Elderly with Dental Caries and Health. Front Cell Infect Microbiol. 2018;8:442.","journal-title":"Front Cell Infect Microbiol"},{"issue":"1","key":"547_CR33","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1111\/j.1600-0765.2011.01409.x","volume":"47","author":"FR Teles","year":"2012","unstructured":"Teles FR, Teles RP, Uzel NG, Song XQ, Torresyap G, Socransky SS, et al. Early microbial succession in redeveloping dental biofilms in periodontal health and disease. J Periodontal Res. 2012;47(1):95\u2013104.","journal-title":"J Periodontal Res"},{"key":"547_CR34","doi-asserted-by":"publisher","first-page":"423","DOI":"10.3389\/fcimb.2018.00423","volume":"8","author":"C Zhu","year":"2018","unstructured":"Zhu C, Yuan C, Ao S, Shi X, Chen F, Sun X, et al. The predictive potentiality of salivary microbiome for the recurrence of early childhood caries. Front Cell Infect Microbiol. 2018;8:423.","journal-title":"Front Cell Infect Microbiol"},{"issue":"1","key":"547_CR35","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1177\/0022034515609554","volume":"95","author":"I Johansson","year":"2016","unstructured":"Johansson I, Witkowska E, Kaveh B, Lif Holgerson P, Tanner AC. The Microbiome in populations with a low and high prevalence of Caries. J Dent Res. 2016;95(1):80\u20136.","journal-title":"J Dent Res"},{"issue":"2","key":"547_CR36","doi-asserted-by":"publisher","first-page":"127","DOI":"10.33073\/pjm-2014-018","volume":"63","author":"I Struzycka","year":"2014","unstructured":"Struzycka I. The oral microbiome in dental caries. Pol J Microbiol. 2014;63(2):127\u201335.","journal-title":"Pol J Microbiol"},{"issue":"10","key":"547_CR37","doi-asserted-by":"publisher","first-page":"878","DOI":"10.1177\/154405910608501002","volume":"85","author":"AF Paes Leme","year":"2006","unstructured":"Paes Leme AF, Koo H, Bellato CM, Bedi G, Cury JA. The role of sucrose in cariogenic dental biofilm formation\u2013new insight. J Dent Res. 2006;85(10):878\u201387.","journal-title":"J Dent Res"},{"issue":"5","key":"547_CR38","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1007\/s11274-024-03971-7","volume":"40","author":"JR Zhao","year":"2024","unstructured":"Zhao JR, Zuo SQ, Xiao F, Guo FZ, Chen LY, Bi K, et al. Advances in biotin biosynthesis and biotechnological production in microorganisms. World J Microbiol Biotechnol. 2024;40(5):163.","journal-title":"World J Microbiol Biotechnol"},{"key":"547_CR39","doi-asserted-by":"publisher","first-page":"503","DOI":"10.1146\/annurev-micro-111021-053553","volume":"76","author":"JK Teschler","year":"2022","unstructured":"Teschler JK, Nadell CD, Drescher K, Yildiz FH. Mechanisms underlying Vibrio cholerae Biofilm formation and dispersion. Annu Rev Microbiol. 2022;76:503\u201332.","journal-title":"Annu Rev Microbiol"},{"key":"547_CR40","doi-asserted-by":"publisher","first-page":"169628","DOI":"10.1016\/j.scitotenv.2023.169628","volume":"913","author":"R Xiao","year":"2024","unstructured":"Xiao R, Hu Y, Wang Y, Li J, Guo C, Bai J, et al. Pathogen profile of Baiyangdian Lake sediments using metagenomic analysis and their correlation with environmental factors. Sci Total Environ. 2024;913:169628.","journal-title":"Sci Total Environ"},{"key":"547_CR41","doi-asserted-by":"publisher","first-page":"172242","DOI":"10.1016\/j.scitotenv.2024.172242","volume":"927","author":"X Chen","year":"2024","unstructured":"Chen X, Yang G, Quan X, Zhu S, Qin B, Shou D, et al. Significance of a minor pilin PilV in biofilm cohesion of Geobacter sulfurreducens. Sci Total Environ. 2024;927:172242.","journal-title":"Sci Total Environ"},{"key":"547_CR42","doi-asserted-by":"publisher","first-page":"127149","DOI":"10.1016\/j.micres.2022.127149","volume":"264","author":"L Ma","year":"2022","unstructured":"Ma L, Feng J, Zhang J, Lu X. Campylobacter biofilms. Microbiol Res. 2022;264:127149.","journal-title":"Microbiol Res"},{"issue":"1","key":"547_CR43","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1002\/jobm.201100335","volume":"53","author":"Q Duan","year":"2013","unstructured":"Duan Q, Zhou M, Zhu L, Zhu G. Flagella and bacterial pathogenicity. J Basic Microbiol. 2013;53(1):1\u20138.","journal-title":"J Basic Microbiol"}],"container-title":["Progress in Orthodontics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s40510-024-00547-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s40510-024-00547-x\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s40510-024-00547-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,15]],"date-time":"2024-12-15T20:01:48Z","timestamp":1734292908000},"score":1,"resource":{"primary":{"URL":"https:\/\/progressinorthodontics.springeropen.com\/articles\/10.1186\/s40510-024-00547-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,16]]},"references-count":43,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["547"],"URL":"https:\/\/doi.org\/10.1186\/s40510-024-00547-x","relation":{},"ISSN":["2196-1042"],"issn-type":[{"value":"2196-1042","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,16]]},"assertion":[{"value":"5 June 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 November 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 December 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This study followed the STROBE guidelines after obtaining approval from the Research Ethics Committee at the Stomatological Hospital, School of Stomatology, Southern Medical University (Ref No: EC-CT-[2022]23).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors certify that they have no commercial or associative interest in connection with the manuscript.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"48"}}