{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T20:17:05Z","timestamp":1782505025248,"version":"3.54.5"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T00:00:00Z","timestamp":1698019200000},"content-version":"vor","delay-in-days":295,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T00:00:00Z","timestamp":1698019200000},"content-version":"vor","delay-in-days":295,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Nippon Sigmax Co., Ltd."}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J. exp. orthop."],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>Osteoarthritis (OA) is a combination of degeneration and destruction of articular cartilage due to mechanical stress, secondary synovitis, and bone remodelling. In recent years, early knee OA, a preliminary stage of structural failure in OA, has attracted attention as a potential target for therapy to prevent the onset of OA. Intra\u2010articular administration of monoiodoacetic acid (MIA) induces OA\u2010like symptoms, and low doses of MIA induce early OA like symptoms. In this experiment, a low\u2010dose of MIA was induced to early OA model mice, which were then irradiated with low\u2010intensity pulsed ultrasound (LIPUS) to examine whether LIPUS improves symptoms of early OA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>After 4 weeks of LIPUS irradiation, articular cartilage was observed at 1 and 4 weeks. The Osteoarthritis Research Society International (OARSI) scores were calculated using Safranin\u2010O staining results. Cartilage degeneration was detected using Denatured Collagen Detection Reagent (DCDR).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We observed a significant decrease in OARSI scores in the LIPUS irradiated group at week 4. The non\u2010LIPUS group showed widespread areas of double positivity for Type II collagen and DCDR, whereas the LIPUS group showed only a small number of DCDR\u2010positive areas. In addition, macrophage numbers counted in the articular capsule at week 1 showed a significant decrease in the LIPUS irradiated group. Lubricin detection showed that lubricin positive cell number was significantly increased by LIPUS irradiation at week 4.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results suggest that LIPUS attenuates cartilage degeneration in early OA by relieving inflammation and enhancing the inhibitory effect of lubricin on cartilage degeneration.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s40634-023-00672-2","type":"journal-article","created":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T01:01:46Z","timestamp":1698022906000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Low\u2010intensity pulsed ultrasound irradiation attenuates collagen degradation of articular cartilage in early osteoarthritis\u2010like model mice"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8012-5935","authenticated-orcid":false,"given":"Yoshitsugu","family":"Kojima","sequence":"first","affiliation":[{"name":"Clinical Pharmacology Research Laboratory Yokohama University of Pharmacy 601\u00a0Matanocho Totsukaku 245\u20100066 Yokohama Kanagawa Japan"},{"name":"Planning and Product Development Division Nippon Sigmax Co. Ltd. 7th Floor, 1\u201024\u20101\u00a0Nishi\u2010shinjuku 160\u20100023 Shinjuku\u2010Ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takayuki","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Clinical Pharmacology Research Laboratory Yokohama University of Pharmacy 601\u00a0Matanocho Totsukaku 245\u20100066 Yokohama Kanagawa Japan"},{"name":"Planning and Product Development Division Nippon Sigmax Co. 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