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However, the impact of this combination is still under investigation. We compared the effects of the combination of mild hypothermia or normothermia with propofol or dexmedetomidine on brain, lung, and kidney in experimental AIS. AIS-induced Wistar rats (<jats:italic>n<\/jats:italic>\u2009=\u200930) were randomly assigned, after 24 h, to normothermia or mild hypothermia (32\u201335\u00a0\u00b0C) with propofol or dexmedetomidine. Histologic injury score and molecular biomarkers were evaluated not only in brain, but also in lung and kidney. Hemodynamics, ventilatory parameters, and carotid Doppler ultrasonography were analyzed for 60 min.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>In brain: (1) hypothermia compared to normothermia, regardless of sedative, decreased tumor necrosis factor (TNF)-\u03b1 expression and histologic injury score; (2) normothermia\u2009+\u2009dexmedetomidine reduced TNF-\u03b1 and histologic injury score compared to normothermia\u2009+\u2009propofol; (3) hypothermia\u2009+\u2009dexmedetomidine increased zonula occludens-1 expression compared to normothermia\u2009+\u2009dexmedetomidine. In lungs: (1) hypothermia\u2009+\u2009propofol compared to normothermia\u2009+\u2009propofol reduced TNF-\u03b1 and histologic injury score; (2) hypothermia\u2009+\u2009dexmedetomidine compared to normothermia\u2009+\u2009dexmedetomidine reduced histologic injury score. In kidneys: (1) hypothermia\u2009+\u2009dexmedetomidine compared to normothermia\u2009+\u2009dexmedetomidine decreased syndecan expression and histologic injury score; (2) hypothermia\u2009+\u2009dexmedetomidine compared to hypothermia\u2009+\u2009propofol decreased histologic injury score.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>In experimental AIS, the combination of mild hypothermia with dexmedetomidine reduced brain, lung, and kidney damage.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s40635-022-00481-4","type":"journal-article","created":{"date-parts":[[2022,12,19]],"date-time":"2022-12-19T00:04:33Z","timestamp":1671408273000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Mild hypothermia combined with dexmedetomidine reduced brain, lung, and kidney damage in experimental acute focal ischemic stroke"],"prefix":"10.1186","volume":"10","author":[{"given":"Denise","family":"Battaglini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana Lopes","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathane Santanna","family":"Felix","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gisele","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Alves","family":"Antunes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazareth Novaes","family":"Rocha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera Luiza","family":"Capelozzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo Marcos","family":"Morales","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda Ferreira","family":"Cruz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Robba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro Leme","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Pelosi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1412-7136","authenticated-orcid":false,"given":"Patricia Rieken Macedo","family":"Rocco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,12,19]]},"reference":[{"key":"481_CR1","doi-asserted-by":"publisher","first-page":"1247","DOI":"10.1016\/S0140-6736(18)31874-9","volume":"392","author":"C Zerna","year":"2018","unstructured":"Zerna C, Thomalla G, Campbell BCV et al (2018) Current practice and future directions in the diagnosis and acute treatment of ischaemic stroke. 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