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Bioprocess."],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Although metal\u2013organic frameworks (MOFs) have been considered as promising matrices for enzyme immobilization, HKUST-1, constructed from copper acetate (CuAc<jats:sub>2<\/jats:sub>) and benzene 1,3,5-tricarboxylate (BTC), has rarely been explored for this application. In this study, mushroom tyrosinase (EC 1.14.18.1) was immobilized in the form of tyrosinase@HKUST-1 following a simple reaction procedure by\u00a0mixing BTC with the enzyme prior to addition of CuAc<jats:sub>2<\/jats:sub>. The resultant biocatalyst was characterized in both structural features and catalytic properties. Upon incorporation into the HKUST-1 frameworks, the enzyme gained a prominent enhancement in stability against pH, temperature and storage: When incubated at 50\u00a0\u00b0C and pH 6.0, tyrosinase@HKUST-1 presented a half-life of 32.6\u00a0h, which is 77-fold and over tenfold higher than that of the free enzyme and its other immobilization forms, respectively; and the catalyst fully maintained its activity for at least 2\u00a0months when stored at 30\u00a0\u00b0C. The applicability of this new biocatalyst was demonstrated by employing it as catalyst for regioselective<jats:italic>ortho<\/jats:italic>-hydroxylation reactions to produce catecholic products with huge pharmacological effects, i.e., hydroxytyrosol and<jats:sc>l<\/jats:sc>-DOPA, with excellent yields and productivities. This study has thus offered a facile immobilization method to prepare a novel biocatalyst with super stability, and tyrosinase@HKUST-1 so formed from crude mushroom extract provides an efficient catalyst which can be applied to the production of catecholic products with health benefits.<\/jats:p><jats:p><jats:bold>Graphical Abstract<\/jats:bold><\/jats:p>","DOI":"10.1186\/s40643-021-00462-7","type":"journal-article","created":{"date-parts":[[2021,10,27]],"date-time":"2021-10-27T03:32:21Z","timestamp":1635305541000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Tyrosinase@HKUST-1: a super stable biocatalyst efficient for catecholic product synthesis"],"prefix":"10.1186","volume":"8","author":[{"given":"Xiao-Feng","family":"L\u00fc","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao-Yun","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuangfei","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guo-Hao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4594-9271","authenticated-orcid":false,"given":"Zhen","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,10,27]]},"reference":[{"key":"462_CR1","doi-asserted-by":"publisher","first-page":"1119","DOI":"10.1007\/s00253-014-6310-6","volume":"99","author":"Y Achmon","year":"2015","unstructured":"Achmon Y, Fishman A (2015) The antioxidant hydroxytyrosol: biotechnological production challenges and opportunities. 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