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Bioprocess."],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The biomass pretreatment strategies using organic acids facilitate lignin removal and enhance the enzymatic digestion of cellulose. However, lignin always suffers a severe and irreversible condensation. The newly generated C\u2013C bonds dramatically affect its further upgrading. In this study, we used a recyclable hydrotrope (\n                    <jats:italic>p<\/jats:italic>\n                    -Toluenessulfonic acid,\n                    <jats:italic>p<\/jats:italic>\n                    -TsOH) to dissolve lignin under mild condition and stabilized lignin with a quenching agent (formaldehyde, FA) during extraction, achieving both value-added lignin extraction and efficient enzymatic saccharification of cellulose. Approximately 63.7% of lignin was dissolved by 80% (wt. %)\n                    <jats:italic>p<\/jats:italic>\n                    -TsOH with 1.5% FA addition at 80\u00a0\u00b0C, 30\u00a0min. The obtained lignin was characterized by FTIR spectroscopy, TGA, 2D HSQC NMR spectroscopy, and GPC. The results indicated that the extracted lignin exhibited excellent properties, such as light color, a low molecular weight (\n                    <jats:italic>M<\/jats:italic>\n                    w, 5371\u00a0g\/mol), and a narrow polydispersity (\n                    <jats:italic>M<\/jats:italic>\n                    <jats:sub>w<\/jats:sub>\n                    \/\n                    <jats:italic>M<\/jats:italic>\n                    <jats:sub>n<\/jats:sub>\n                    , 1.63). The pretreated substrate was converted to ethanol via a quasi-simultaneous saccharification and fermentation process (Q-SSF). After fermentation of 60\u00a0h, the ethanol concentration reached 38.7\u2009\u00b1\u20093.3\u00a0g\/L which was equivalent to a theoretical ethanol yield of 82.9\u2009\u00b1\u20092.2% based on the glucan content, while the residual glucose concentration was only 4.69\u2009\u00b1\u20091.4\u00a0g\/L. In short, this pretreatment strategy protected lignin to form new C\u2013C linkages and improved the enzymatic saccharification of glucan for high-titer ethanol production.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Graphical Abstract<\/jats:bold>\n                  <\/jats:p>","DOI":"10.1186\/s40643-022-00530-6","type":"journal-article","created":{"date-parts":[[2022,4,4]],"date-time":"2022-04-04T01:02:53Z","timestamp":1649034173000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["A hydrotrope pretreatment for stabilized lignin extraction and high titer ethanol production"],"prefix":"10.1186","volume":"9","author":[{"given":"Hairui","family":"Ji","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Furong","family":"Tao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhipeng","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuezhi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cuihua","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqiang","family":"Pang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,4,4]]},"reference":[{"issue":"4","key":"530_CR1","doi-asserted-by":"publisher","first-page":"501","DOI":"10.1007\/s00449-017-1884-x","volume":"41","author":"GG Chong","year":"2018","unstructured":"Chong GG, Huang XJ, Di JH, Xu DZ, He YC, Pei YN, Tang YJ, Ma CL (2018) Biodegradation of alkali lignin by a newly isolated Rhodococcus pyridinivorans CCZU-B16. 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