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Bioprocess."],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Blasting extrusion pretreatment (BEP) was evaluated for obtaining sugar enriched saccharified extracts from sweet sorghum bagasse (SSB) for ethanol fermentation. A factorial design was used for studying the effect of last barrel zone temperature (T\n                    <jats:sub>LBZ<\/jats:sub>\n                    ) and screw configuration (SC) on carbohydrate release and extraction yield. The experiment with a higher total carbohydrate release was selected (BEP\u2009+\u2009ES) for a posterior 72\u00a0h fermentation and compared against enzymatic saccharified (ES) SSB without using BEP. Fermentation was performed at 28\u00a0\u00b0C, pH 5.0 and 10% of solids loading (BEP\u2009+\u2009ES and ES) using the stress tolerant yeast\n                    <jats:italic>Pichia kudriavzevii<\/jats:italic>\n                    ATCC 203,081 for ethanol production. In BEP experiments, screw configuration with one polygon element and a shear zone consisting of 7 reverse elements operating at a T\n                    <jats:sub>LBZ<\/jats:sub>\n                    of 190\u00a0\u00b0C resulted in the highest carbohydrate release after enzymatic saccharification, achieving a 3-fold increase compared to control. BEP enhanced carbohydrate availability and lignocellulosic biomass degradation activity (enzymatic saccharification and\n                    <jats:italic>P. kudravzevii<\/jats:italic>\n                    fermentation). Ethanol production usining\n                    <jats:italic>P. kudriavzevii<\/jats:italic>\n                    with BEP\u2009+\u2009ES was 8.65-fold higher than the ES control after 72-h fermentation. Higher initial total carbohydrate (3-fold) and fermentable amino nitrogen (FAN) in BEP\u2009+\u2009ES (12.41-fold higher) improved SSB fermentability and thus ethanol yield. Glucose was fully consumed in the BEP\u2009+\u2009ES fermentation, while 91.62% was consumed in the ES experiment. Xylose and mannose\/arabinose consumption varied by treatment, but\n                    <jats:italic>P. kudriavzevvi<\/jats:italic>\n                    displayed the ability to co-utilize pentoses and hexoses during fermentation. Compared to previous traditional twin-screw extrusion and ES, BEP enhanced SSB carbohydrate release during enzymatic saccharification, and carbohydrate consumption and ethanol production during fermentation.\n                  <\/jats:p>","DOI":"10.1186\/s40643-025-00905-5","type":"journal-article","created":{"date-parts":[[2025,6,19]],"date-time":"2025-06-19T05:48:31Z","timestamp":1750312111000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Blasting extrusion pretreatment of sweet sorghum bagasse for enhanced enzymatic saccharification and ethanol production using Pichia kudriavzevii ATCC 20,381"],"prefix":"10.1186","volume":"12","author":[{"given":"Benjam\u00edn","family":"V\u00e1zquez-Rodr\u00edguez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erick","family":"Heredia-Olea","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana","family":"Alamilla-Morales","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2636-6281","authenticated-orcid":false,"given":"Esther","family":"P\u00e9rez-Carrillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Perez-Perez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio O.","family":"Serna-Sald\u00edvar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,6,19]]},"reference":[{"key":"905_CR2","doi-asserted-by":"publisher","unstructured":"\u00c1lvaro HM, Moura J, Rodrigues EAB, Kleingesinds D, Rodrigues EK, R.C.L.B (2022) A residue-free and effective corncob extrusion pretreatment for the enhancement of high solids loading enzymatic hydrolysis to produce sugars. 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