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Bioprocess."],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Tobacco aging is a critical for developing desirable flavor profiles, driven primarily by microbial and enzymatic activities. This study systematically evaluated the effects of\n                    <jats:italic>Bacillus clausii<\/jats:italic>\n                    inoculation (FJ) and\n                    <jats:italic>B. clausii<\/jats:italic>\n                    -cellulase co-treatment (JM) on the surface microbial communities and aroma compounds of Yunyan 87 tobacco leaves during 9\u00a0months of aging, with natural aging as the control (CK). High-throughput 16S rRNA sequencing revealed that the JM treatment significantly increased microbial diversity and enhanced the structural similarity of microbial communities across different aging stages compared with the CK and FJ treatments, while also promoting the proliferation of beneficial taxa (e.g.,\n                    <jats:italic>Bacteroidota<\/jats:italic>\n                    ,\n                    <jats:italic>Cyanobacteria<\/jats:italic>\n                    ). PICRUSt functional prediction revealed that JM enriched metabolic pathways related to carbohydrate metabolism (18.2\u201320.6%), amino acid metabolism (15.1\u201316.7%), and metabolism of cofactors and vitamins (16.5\u201317.7%)\u2013key pathways for flavor precursor conversion. A total of 29 key aroma compounds (odor activity value\u2009&gt;\u20091) were significantly influenced the quality of tobacco leaves, with JM significantly increasing total volatile content (317.1\u2013388.8\u00a0\u03bcg\/g vs. 124.7\u2013143.0\u00a0\u03bcg\/g in CK and 283.2\u2013300.8\u00a0\u03bcg\/g in FJ). Notably, JM promoted the accumulation of esters (ethyl palmitate, ethyl linoleate) and ketones (4,7,9-megastigmatrien-3-one, damascenone), contributing desirable fruity, floral, and sweet notes. Correlation analysis linked\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    ,\n                    <jats:italic>Bacillus<\/jats:italic>\n                    , and\n                    <jats:italic>Acinetobacter<\/jats:italic>\n                    to the formation of key volatiles. These findings demonstrate that microbial-enzyme co-fermentation enriched desirable aromas, and stabilized microbial communities, providing a practical and efficient strategy for industrial production of high-quality aged tobacco.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Graphical abstract<\/jats:bold>\n                  <\/jats:p>","DOI":"10.1186\/s40643-026-01052-1","type":"journal-article","created":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T01:51:17Z","timestamp":1776217877000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Microbial-enzyme synergistic treatment stabilizes surface microbial communities and enhances flavor quality during tobacco leaf aging"],"prefix":"10.1186","volume":"13","author":[{"given":"Chunping","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yizhe","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuntao","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qu","family":"Lili","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meizhou","family":"Ding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma","family":"Rong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,4,15]]},"reference":[{"issue":"1","key":"1052_CR1","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1186\/s40643-025-00969-3","volume":"12","author":"W Cai","year":"2025","unstructured":"Cai W, Zhang QY, Zhu PC, Hu WR, An HY, Huo DQ, Li DL (2025) Study on dynamic changes and correlations of microbial diversity, key enzyme activity and conventional components in tobacco leaves during the aging process. 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