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This pathogen activates multiple regulatory mechanisms in response to stress, and cobalamin biosynthesis might have a potential role in bacterial protection. Low temperature is a strategy used in the food industry to control bacteria proliferation; however, <jats:italic>L. monocytogenes<\/jats:italic> can grow in cold temperatures and overcome different stress conditions. In this study we selected <jats:italic>L. monocytogenes<\/jats:italic> List2-2, a strain with high tolerance to the combination of low temperature\u2009+\u2009copper, to understand whether the cobalamin biosynthesis pathway is part of the tolerance mechanism to this stress condition. For this, we characterized the transcription level of three cobalamin biosynthesis-related genes (<jats:italic>cbiP<\/jats:italic>, <jats:italic>cbiB,<\/jats:italic> and <jats:italic>cysG<\/jats:italic>) and the <jats:italic>eutV<\/jats:italic> gene, a transcriptional regulator encoding gene involved in ethanolamine metabolism, in <jats:italic>L. monocytogenes<\/jats:italic> strain List2-2 growing simultaneously under two environmental stressors: low temperature (8\u00a0\u00b0C)\u2009+\u2009copper (0.5\u00a0mM of CuSO<jats:sub>4<\/jats:sub>\u2009\u00d7\u20095H<jats:sub>2<\/jats:sub>O). In addition, the gene <jats:italic>cbiP<\/jats:italic>, which encodes an essential cobyric acid synthase required in the cobalamin pathway, was deleted by homologous recombination to evaluate the impact of this gene in <jats:italic>L. monocytogenes<\/jats:italic> tolerance to a low temperature (8\u00a0\u00b0C)\u2009+\u2009different copper concentrations.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>By analyzing the KEGG pathway database, twenty-two genes were involved in the cobalamin biosynthesis pathway in <jats:italic>L. monocytogenes<\/jats:italic> List2-2. The expression of genes <jats:italic>cbiP<\/jats:italic>, <jats:italic>cbiB,<\/jats:italic> and <jats:italic>cysG,<\/jats:italic> and <jats:italic>eutV<\/jats:italic> increased 6\u00a0h after the exposure to low temperature\u2009+\u2009copper. The cobalamin <jats:italic>cbiP<\/jats:italic> mutant strain List2-2\u0394<jats:italic>cbiP<\/jats:italic> showed less tolerance to low temperature\u2009+\u2009copper (3\u00a0mM) than the wild-type <jats:italic>L. monocytogenes<\/jats:italic> List2-2. The addition of cyanocobalamin (5\u00a0nM) to the medium reverted the phenotype observed in List2-2\u0394<jats:italic>cbiP<\/jats:italic>.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>These results indicate that cobalamin biosynthesis is necessary for <jats:italic>L. monocytogenes<\/jats:italic> growth under stress and that the <jats:italic>cbiP<\/jats:italic> gene may play a role in the survival and growth of <jats:italic>L. monocytogenes<\/jats:italic> List2-2 at low temperature\u2009+\u2009copper.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s40659-022-00376-4","type":"journal-article","created":{"date-parts":[[2022,3,2]],"date-time":"2022-03-02T10:04:17Z","timestamp":1646215457000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Cobalamin cbiP mutant shows decreased tolerance to low temperature and copper stress in Listeria monocytogenes"],"prefix":"10.1186","volume":"55","author":[{"given":"L.","family":"V\u00e1squez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Parra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. 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