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Despite advancements, effective strategies for eradicating these biofilms remain elusive. While high-intensity focused ultrasound (HIFU) has been popularized in medicine, its effects on dental implant-attached biofilms remain unclear. This study presents in vitro findings on the effects of HIFU treatment on titanium (Ti)-attached <jats:italic>Streptococcus mutans<\/jats:italic> (<jats:italic>S. mutans<\/jats:italic>) biofilms and evaluates its impacts on the surface roughness and chemical composition of the Ti disc substrates.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>To optimise the HIFU parameters, four quadrants of a pair of Ti discs [machined (M) and alumina grit blasted (AB)] were marked using laser etching (MD Waterlase, US). HIFU beams, generated by a 254\u00a0kHz transducer and operated at intensities of 0 W, 10 W, 20 W, and 30 W, were applied to each quadrant for 2\u00a0min (min) in a water medium. The roughness of the treated surfaces was measured using Atomic Force Microscopy (AFM), and the surface composition was analyzed using Scanning Electron Microscope\u2013Energy Dispersive Spectrometry (SEM\u2013EDS). To investigate the biofilm debridement, 10-day-old <jats:italic>S. mutans<\/jats:italic> cultures were grown on 20 pairs of similar Ti discs, and then the optimized HIFU intensity of 20W was applied to five test pairs. Qualitative analyses were performed using a Dual Fluorescence\/Reflection Confocal Laser Scanning Microscope (FRCLSM) and SEM imaging. Quantitative data on cell viability were collected using crystal violet (CV), (3-[4,5-dimethylthiazol-2-yl]-2,5 2,5-diphenyl tetrazolium bromide) (MTT), and flow cytometry (FCM) assays. Data from these test conditions were analyzed alongside cultures on biofilms that were untreated (control). Statistical data were calculated using ANOVA and appropriate <jats:italic>t<\/jats:italic>-tests for repeated measures.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>The surface roughness of AB Ti discs showed a highest and significant increase (p\u2009&lt;\u20090.05) following HIFU exposure at 20 W through three roughness parameters (Sa, Sq, and Sdr), compared to the controls (1207\u00a0nm, 1455\u00a0nm, 62% compared to 842\u00a0nm, 1042\u00a0nm and 30% respectively). This optimized HIFU treatment not only significantly reduced the bacterial counts of the biofilms (76% of total bacteria from M discs, 59% on AB discs in FCM assays) but also created areas of complete biofilm removal in SEM images.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>This study provides preliminary in vitro evidence that HIFU can remove bacterial biofilms. Further research is required to determine its feasibility as a potential non-surgical approach for the prevention and management of peri-implantitis.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/s40729-025-00645-3","type":"journal-article","created":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T12:09:56Z","timestamp":1756728596000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["High-intensity focused ultrasound for biofilm debridement, an in vitro proof-of-concept using Ti-attached Streptococcus mutans"],"prefix":"10.1186","volume":"11","author":[{"given":"Minh Dien","family":"Tran","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheetal Maria","family":"Rajan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hien Chi","family":"Ngo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amr","family":"Fawzy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,9,1]]},"reference":[{"issue":"Suppl 20","key":"645_CR1","first-page":"S246","volume":"45","author":"F Schwarz","year":"2018","unstructured":"Schwarz F, et al. 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