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Microarray studies have linked many genes to pancreatic cancer, but the available data have not been extensively mined for potential insights into PDAC. This study attempted to identify PDAC\u2010associated genes and signaling pathways based on six microarray\u2010based profiles of gene expression in pancreatic cancer deposited in the gene expression omnibus database.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p xml:lang=\"en\">\n                      Pathway network methods were used to analyze core pathways in six publicly available pancreatic cancer gene (GSE71989, GSE15471, GSE16515, GSE32676, GSE41368 and GSE28735) expression profiles. Genes potentially linked to PDAC were assessed for potential impact on survival time based on data in The Cancer Genome Atlas and International Cancer Genome Consortium databases, and the expression of one candidate gene (\n                      <jats:italic toggle=\"yes\">CKS2<\/jats:italic>\n                      ) and its association with survival was examined in 102 patients with PDAC from our hospital. Effects of\n                      <jats:italic toggle=\"yes\">CKS2<\/jats:italic>\n                      knockdown were explored in the PDAC cell lines BxPC\u20103 and CFPAC\u20101.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p xml:lang=\"en\">\n                      The KEGG signaling pathway called \u201cpathway in cancer\u201d may play an important role in pancreatic cancer development and progression. Five genes (\n                      <jats:italic toggle=\"yes\">BIRC5<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">CKS2<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">ITGA3<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">ITGA6<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">RALA<\/jats:italic>\n                      ) in this pathway were significantly associated with survival time in patients with PDAC.\n                      <jats:italic toggle=\"yes\">CKS2<\/jats:italic>\n                      was overexpressed in PDAC samples from our hospital, and higher\n                      <jats:italic toggle=\"yes\">CKS2<\/jats:italic>\n                      expression in these patients was associated with shorter survival time.\n                      <jats:italic toggle=\"yes\">CKS2<\/jats:italic>\n                      knockdown substantially inhibited PDAC cell proliferation in vitro.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p xml:lang=\"en\">Analysis integrating existing microarray datasets allowed identification of the \u201cpathway in cancer\u201d as an important signaling pathway in PDAC. This integrative approach may be powerful for identifying genes and pathways involved in cancer.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s40880-018-0289-9","type":"journal-article","created":{"date-parts":[[2018,4,26]],"date-time":"2018-04-26T23:08:52Z","timestamp":1524784132000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Integrative analysis of gene expression profiles reveals specific signaling pathways associated with pancreatic duct adenocarcinoma"],"prefix":"10.34133","volume":"38","author":[{"given":"Jun","family":"Li","sequence":"first","affiliation":[{"name":"Chinese Academy of Medical Sciences and Peking Union Medical College","place":["Beijing, P.R. China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenle","family":"Tan","sequence":"additional","affiliation":[{"name":"Chinese Academy of Medical Sciences and Peking Union Medical College","place":["Beijing, P.R. China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linna","family":"Peng","sequence":"additional","affiliation":[{"name":"Chinese Academy of Medical Sciences and Peking Union Medical College","place":["Beijing, P.R. China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jialiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Sun Yat\u2010sen University Cancer Center","place":["Guangzhou, P.R. China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xudong","family":"Huang","sequence":"additional","affiliation":[{"name":"Chinese Academy of Medical Sciences and Peking Union Medical College","place":["Beijing, P.R. China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qionghua","family":"Cui","sequence":"additional","affiliation":[{"name":"Chinese Academy of Medical Sciences and Peking Union Medical College","place":["Beijing, P.R. China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Zheng","sequence":"additional","affiliation":[{"name":"Sun Yat\u2010sen University Cancer Center","place":["Guangzhou, P.R. China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen","family":"Tan","sequence":"additional","affiliation":[{"name":"Chinese Academy of Medical Sciences and Peking Union Medical College","place":["Beijing, P.R. China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Wu","sequence":"additional","affiliation":[{"name":"Chinese Academy of Medical Sciences and Peking Union Medical College","place":["Beijing, P.R. China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongxin","family":"Lin","sequence":"additional","affiliation":[{"name":"Chinese Academy of Medical Sciences and Peking Union Medical College","place":["Beijing, P.R. China"]},{"name":"Sun Yat\u2010sen University Cancer Center","place":["Guangzhou, P.R. China"]},{"name":"Nanjing Medical University","place":["Nanjing, P.R. China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","published-online":{"date-parts":[[2018,4,27]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13000\u2010016\u20100497\u2010z"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21332"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21338"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki022"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102164\u201015\u201013"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1136678"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/bfgp\/elv017"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u201062703\u2010721\u20101_9"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.211"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0074250"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000658\u2010200107000\u201000003"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13402\u2010016\u20100283\u20107"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.15068"},{"key":"e_1_3_2_15_2","first-page":"2016","article-title":"Combined gene expression analysis of whole\u2010tissue and microdissected pancreatic ductal adenocarcinoma identifies genes specifically overexpressed in tumor epithelia","volume":"55","author":"Badea L","year":"2008","unstructured":"Badea L, Herlea V, Dima SO, Dumitrascu T, Popescu I. 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