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This study was undertaken to evaluate larvicidal activities of 80% methanol and n-hexane extracts of four plants that are traditionally used by communities against mosquitoes\n                      <jats:italic>.<\/jats:italic>\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The dried plant parts of\n                      <jats:italic>Ocimum lamiifolium<\/jats:italic>\n                      ,\n                      <jats:italic>Amaranthus hybridus, Premna schimperi<\/jats:italic>\n                      , and\n                      <jats:italic>Lepidium sativum<\/jats:italic>\n                      were extracted with 80% methanol and n-hexane solvents. Bioinsecticidal activities of these extracts were evaluated under laboratory condition in the range of 62.5\u20132000\u00a0ppm against late 3rd to early 4th instar larvae of\n                      <jats:italic>An. arabiensis, An. stephensi<\/jats:italic>\n                      and\n                      <jats:italic>Ae. aegypti<\/jats:italic>\n                      mosquitoes. Larval mortality was observed after 24\u00a0h of exposure. The mortality data were subjected to probit analysis to determine LC\n                      <jats:sub>50<\/jats:sub>\n                      and LC\n                      <jats:sub>90<\/jats:sub>\n                      values.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In the concentration ranges of 62.5\u20132000\u00a0ppm, the LC\n                      <jats:sub>50<\/jats:sub>\n                      and LC\n                      <jats:sub>90<\/jats:sub>\n                      values of the most potent n-hexane extracts tested plants;\n                      <jats:italic>Ocimum lamiifolium<\/jats:italic>\n                      against\n                      <jats:italic>An. arabiensis, An. stephensi<\/jats:italic>\n                      and\n                      <jats:italic>Ae. aegypti<\/jats:italic>\n                      with a general ranges 666.07 to 1278.22, and 1920.82 to 2139.91\n                      <jats:italic>,<\/jats:italic>\n                      and\n                      <jats:italic>Amaranthus hybridus<\/jats:italic>\n                      against\n                      <jats:italic>An. stephensi<\/jats:italic>\n                      and\n                      <jats:italic>Ae. aegypti<\/jats:italic>\n                      412 to 1426.03 and 736.150 to 1222.62\n                      <jats:italic>, Lepidium sativum<\/jats:italic>\n                      and\n                      <jats:italic>Premna schimperi<\/jats:italic>\n                      against\n                      <jats:italic>An. arabiensis<\/jats:italic>\n                      exhibited 100% larvicidal activity with LC\n                      <jats:sub>50<\/jats:sub>\n                      and LC\n                      <jats:sub>90<\/jats:sub>\n                      values ranges 713.25 to 1278.22, and 636.76 to 988.90, respectively. All the n-hexane extracts showed larvicidal activities.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The n-hexane crude extracts of the tested plants have the potential to be used as bioinsecticides against larvae of\n                      <jats:italic>An. arabiensis, An. stephensi<\/jats:italic>\n                      and\n                      <jats:italic>Ae. aegypti<\/jats:italic>\n                      . Therefore, it is necessary to undertake studies that focus on bioassay-guided isolation, purification and structural elucidation of active compound (s) from the most active n-hexane fractions of the tested plants to develop a product that may complement the current existing vector control tools.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s41182-025-00879-2","type":"journal-article","created":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T02:07:33Z","timestamp":1767146853000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Larvicidal effects of selected medicinal plant extracts against Anopheles arabiensis, Anopheles stephensi, and Aedes aegypti"],"prefix":"10.1186","volume":"53","author":[{"given":"Negesse","family":"Gebissa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ketema","family":"Tolossa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Araya","family":"Gebresilassie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esayas","family":"Aklilu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Bisrat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bersissa","family":"Kumsa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sisay","family":"Dugassa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,12,31]]},"reference":[{"key":"879_CR1","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1186\/s13071-025-06761","volume":"18","author":"OC Mbaoma","year":"2025","unstructured":"Mbaoma OC, Thomas SM, Beierkuhnlein C. 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