{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T23:36:03Z","timestamp":1783640163555,"version":"3.55.0"},"reference-count":43,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T00:00:00Z","timestamp":1714953600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T00:00:00Z","timestamp":1714953600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["One Health Outlook"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Diarrheagenic\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      (\n                      <jats:italic>E. coli<\/jats:italic>\n                      ) is a zoonotic pathogen that contaminates abattoir workers, slaughter environments, slaughter equipment, and carcasses during abattoir processing. Infection with\n                      <jats:italic>E. coli<\/jats:italic>\n                      is associated with the consumption of contaminated food and water, and it is a potential threat to the health and welfare of both humans and animals. Hence, this study aimed to detect diarrheagenic\n                      <jats:italic>E. coli<\/jats:italic>\n                      and assess its antibiogram profile in two abattoir settings, in one health lens.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A cross-sectional study in one health approach was conducted from December 2020 to June 2021. A total of 384 samples from abattoir workers\u2019 hands, carcasses, knives, cattle feces, abattoir water and effluents were collected. Bacterial culture and biochemical tests were conducted to isolate\n                      <jats:italic>E. coli<\/jats:italic>\n                      , while conventional polymerase chain reaction was performed to identify virulence genes. The antibiogram of diarrheagenic\n                      <jats:italic>E. coli<\/jats:italic>\n                      was tested against nine antimicrobials using the Kirby Bauer disk diffusion method.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 115 (29.95%)\n                      <jats:italic>E. coli<\/jats:italic>\n                      were isolated from the 384 samples, and from these isolates, about 17 (14.8%) were confirmed to be diarrheagenic\n                      <jats:italic>E. coli<\/jats:italic>\n                      (DEC). Among the DEC pathotypes, nine (52.94%), five (29.4%), and three (17.65%) were Shiga toxin-producing, enterohemorrhagic, and enterotoxigenic\n                      <jats:italic>E. coli<\/jats:italic>\n                      , respectively. While 14 (82.35%) DEC isolates harbored the\n                      <jats:italic>stx2<\/jats:italic>\n                      gene, five (29.41%) the\n                      <jats:italic>eae<\/jats:italic>\n                      gene, five (29.41%) the\n                      <jats:italic>hlyA<\/jats:italic>\n                      gene and three (17.65%) harbored the\n                      <jats:italic>st<\/jats:italic>\n                      gene. All the DEC isolates were resistant to erythromycin and vancomycin; whereas, they were susceptible to ampicillin, nalidixic acid and norfloxacin. Furthermore, 64.7% of DEC isolates showed resistance to both ceftazidime and kanamycin and 88.24% of the isolates showed multidrug resistance.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study detected DEC isolates having different virulence genes, which showed single and multiple antimicrobial resistance. Given the existing poor hygienic and sanitary practices along the abattoir-to-table food chain, coupled with the habit of raw meat consumption, this result indicates a potential public and animal health risk from the pathogen and antimicrobial resistance.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s42522-024-00102-y","type":"journal-article","created":{"date-parts":[[2024,5,5]],"date-time":"2024-05-05T19:02:53Z","timestamp":1714935773000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Detection and antibiogram profile of diarrheagenic Escherichia coli isolated from two abattoir settings in northwest Ethiopia: a one health perspective"],"prefix":"10.1186","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-9464-9029","authenticated-orcid":false,"given":"Solomon Lulie","family":"Abey","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mersha","family":"Teka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5043-1528","authenticated-orcid":false,"given":"Abebe Belete","family":"Bitew","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wassie","family":"Molla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mebrat","family":"Ejo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8773-2698","authenticated-orcid":false,"given":"Gashaw Getaneh","family":"Dagnaw","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takele","family":"Adugna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seleshe","family":"Nigatu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bemrew Admassu","family":"Mengistu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mebrie Zemene","family":"Kinde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adugna","family":"Berju","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1951-2562","authenticated-orcid":false,"given":"Mequanint Addisu","family":"Belete","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wudu","family":"Temesgen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shimelis","family":"Dagnachew","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4103-5029","authenticated-orcid":false,"given":"Tesfaye Sisay","family":"Tesema","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,5,6]]},"reference":[{"key":"102_CR1","doi-asserted-by":"crossref","unstructured":"Pizarro MA, Orozco JH, Degarbo SM, Calder\u00f3n AE, Nardello AL, Laciar A et al. Virulence profiles of Shiga Toxin-Producing Escherichia coli and other potentially diarrheagenic E.coli of bovine origin, in Mendoza, Argentina. Brazilian J Microbiol 2013, 44(4).","DOI":"10.1590\/S1517-83822014005000010"},{"key":"102_CR2","doi-asserted-by":"crossref","unstructured":"Bako E, Kagambega A, Traore KA, Bagre TS, Ibrahim HB, Bouda SC et al. Characterization of Diarrheagenic Escherichia coli Isolated in Organic Waste Products (Cattle Fecal Matter, Manure and, Slurry) from Cattle\u2019s Markets in Ouagadougou, Burkina Faso. Int J Environ Res Public Health 2017, 14(10).","DOI":"10.3390\/ijerph14101100"},{"key":"102_CR3","unstructured":"WHO. World Health Organization. Food Safety and Food Borne Illness, Fact sheet 237. In. Geneva, Switzerland. 2007: 20\u201323."},{"key":"102_CR4","doi-asserted-by":"crossref","unstructured":"Tanih NF, Sekwadi E, Ndip RN, Bessong PO. Detection of pathogenic Escherichia coli and Staphylococcus aureus from cattle and pigs slaughtered in abattoirs in Vhembe District, South Africa. ScientificWorldJournal 2015, 2015:195972.","DOI":"10.1155\/2015\/195972"},{"key":"102_CR5","doi-asserted-by":"crossref","unstructured":"Hassen A, Mumad B, Hiko A, Abraha A. Pathogenic Bacteria Contamination along the beef line at Asella Municipal Abattoir, Arsi Zone Oromia Regional State, Ethiopia. Research Square; 2020.","DOI":"10.21203\/rs.3.rs-54175\/v1"},{"issue":"2","key":"102_CR6","doi-asserted-by":"publisher","first-page":"190","DOI":"10.1016\/j.ijfoodmicro.2012.08.008","volume":"162","author":"C Farrokh","year":"2013","unstructured":"Farrokh C, Jordan K, Auvray F, Glass K, Oppegaard H, Raynaud S, et al. Review of Shiga-toxin-producing Escherichia coli (STEC) and their significance in dairy production. Int J Food Microbiol. 2013;162(2):190\u2013212.","journal-title":"Int J Food Microbiol"},{"issue":"2","key":"102_CR7","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1038\/nrmicro818","volume":"2","author":"JB Kaper","year":"2004","unstructured":"Kaper JB, Nataro JP, Mobley HL. Pathogenic Escherichia coli. Nat Rev Microbiol. 2004;2(2):123\u201340.","journal-title":"Nat Rev Microbiol"},{"key":"102_CR8","unstructured":"FAO\/WHO. Development of Practical Risk Management Strategies based on Microbiological Risk Assessment Outputs. In: Case study: Escherichia coli O157:H7 in fresh raw ground beef. Edited by Butler F, Geraldine Duffy, Dan Engeljohn, Anna M. Lammerding, Tompkin RB, Kiel. Germany; 2006."},{"issue":"627","key":"102_CR9","first-page":"1","volume":"7","author":"JA Odwar","year":"2014","unstructured":"Odwar JA, Kikuvi G, Kariuki JN, Kariuki S. A cross-sectional study on the microbiological quality and safety of raw chicken meats sold in Nairobi, Kenya. BMC Res Notes. 2014;7(627):1\u20138.","journal-title":"BMC Res Notes"},{"issue":"Suppl 1Suppl 1","key":"102_CR10","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/j.bjm.2016.10.015","volume":"47","author":"TA Gomes","year":"2016","unstructured":"Gomes TA, Elias WP, Scaletsky IC, Guth BE, Rodrigues JF, Piazza RM, et al. Diarrheagenic Escherichia coli. Braz J Microbiol. 2016;47(Suppl 1Suppl 1):3\u201330.","journal-title":"Braz J Microbiol"},{"key":"102_CR11","unstructured":"Xia X. PATHOGENIC ESCHERICHIA COLI IN RETAIL MEATS. PhD thesis University of Maryland; 2010."},{"issue":"1","key":"102_CR12","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1016\/j.ijfoodmicro.2013.03.020","volume":"164","author":"A Canizalez-Roman","year":"2013","unstructured":"Canizalez-Roman A, Gonzalez-Nunez E, Vidal JE, Flores-Villasenor H, Leon-Sicairos N. Prevalence and antibiotic resistance profiles of diarrheagenic Escherichia coli strains isolated from food items in northwestern Mexico. Int J Food Microbiol. 2013;164(1):36\u201345.","journal-title":"Int J Food Microbiol"},{"issue":"4","key":"102_CR13","first-page":"255","volume":"20","author":"ZM Taha","year":"2019","unstructured":"Taha ZM, Yassin NA. Prevalence of diarrheagenic Escherichia coli in animal products in Duhok province, Iraq. Iran J Veterinary Res. 2019;20(4):255\u201362.","journal-title":"Iran J Veterinary Res"},{"issue":"1","key":"102_CR14","doi-asserted-by":"publisher","first-page":"305","DOI":"10.21608\/bvmj.2018.54255","volume":"34","author":"SM Saad","year":"2018","unstructured":"Saad SM, Salem AM, Nada S. Hygienic considerations of Pathogenic Escherichia Coli Contamination on Cattle Carcass Surfaces in Egypt. BENHA VETERINARY Med J. 2018;34(1):305\u201313.","journal-title":"BENHA VETERINARY Med J"},{"issue":"4","key":"102_CR15","doi-asserted-by":"publisher","first-page":"464","DOI":"10.7883\/yoken.JJID.2016.486","volume":"70","author":"L Wang","year":"2017","unstructured":"Wang L, Zhang S, Zheng D, Fujihara S, Wakabayashi A, Okahata K, et al. Prevalence of Diarrheagenic Escherichia coli in Foods and fecal specimens obtained from cattle, pigs, chickens, Asymptomatic Carriers, and patients in Osaka and Hyogo, Japan. Jpn J Infect Dis. 2017;70(4):464\u20139.","journal-title":"Jpn J Infect Dis"},{"issue":"3","key":"102_CR16","doi-asserted-by":"publisher","first-page":"282","DOI":"10.1016\/j.ijfoodmicro.2010.01.034","volume":"138","author":"LC Rugeles","year":"2010","unstructured":"Rugeles LC, Bai J, Martinez AJ, Vanegas MC, Gomez-Duarte OG. Molecular characterization of diarrheagenic Escherichia coli strains from stools samples and food products in Colombia. Int J Food Microbiol. 2010;138(3):282\u20136.","journal-title":"Int J Food Microbiol"},{"issue":"3","key":"102_CR17","doi-asserted-by":"publisher","first-page":"196","DOI":"10.1016\/j.ijfoodmicro.2009.06.013","volume":"134","author":"GY Lee","year":"2009","unstructured":"Lee GY, Jang HI, Hwang IG, Rhee MS. Prevalence and classification of pathogenic Escherichia coli isolated from fresh beef, poultry, and pork in Korea. Int J Food Microbiol. 2009;134(3):196\u2013200.","journal-title":"Int J Food Microbiol"},{"issue":"1\u20132","key":"102_CR18","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1016\/j.ijfoodmicro.2011.10.032","volume":"153","author":"A Kagambega","year":"2012","unstructured":"Kagambega A, Martikainen O, Lienemann T, Siitonen A, Traore AS, Barro N, et al. Diarrheagenic Escherichia coli detected by 16-plex PCR in raw meat and beef intestines sold at local markets in Ouagadougou, Burkina Faso. Int J Food Microbiol. 2012;153(1\u20132):154\u20138.","journal-title":"Int J Food Microbiol"},{"key":"102_CR19","unstructured":"CLSI. Performance Standards for Antimicrobial Susceptibility Testing: USA;. 2021."},{"issue":"1","key":"102_CR20","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1186\/s12866-019-1590-8","volume":"19","author":"S Abreham","year":"2019","unstructured":"Abreham S, Teklu A, Cox E, Sisay Tessema T. Escherichia coli O157:H7: distribution, molecular characterization, antimicrobial resistance patterns and source of contamination of sheep and goat carcasses at an export abattoir, Mojdo, Ethiopia. BMC Microbiol. 2019;19(1):215.","journal-title":"BMC Microbiol"},{"key":"102_CR21","doi-asserted-by":"crossref","unstructured":"Haile AF, Kebede D, Wubshet AK. Prevalence and antibiogram of <\u2009i\u2009>\u2009Escherichia coli\u2009O157 isolated from bovine in Jimma, Ethiopia: abattoirbased survey. Ethiop Veterinary J 2017, 21(2).","DOI":"10.4314\/evj.v21i2.8"},{"key":"102_CR22","doi-asserted-by":"crossref","unstructured":"Gutema FD, Abdi RD, Agga GE, Firew S, Rasschaert G, Mattheus W et al. Assessment of beef carcass contamination with Salmonella and E. Coli O 157 in slaughterhouses in Bishoftu, Ethiopia. Int J Food Contam 2021, 8(1).","DOI":"10.1186\/s40550-021-00082-1"},{"key":"102_CR23","doi-asserted-by":"crossref","unstructured":"Ayenew HY, Mitiku BA, Tesema TS. Occurrence of Virulence Genes and Antimicrobial Resistance of E. coli O157:H7 Isolated from the Beef Carcass of Bahir Dar City, Ethiopia. Vet Med Int 2021, 2021:8046680.","DOI":"10.1155\/2021\/8046680"},{"key":"102_CR24","unstructured":"CSA. Centeral Statistical Agency Population Projections for Ethiopia 2007\u20132037. In. Addis Ababa, Ethiopia; 2007."},{"key":"102_CR25","unstructured":"CSA. Centeral Statistical Agency Federal democratic republic of Ethiopia central statistical agency agricultural sample survey. Report on livestock and livestock characteristic. In. Addis Ababa, Ethiopia; 2021."},{"key":"102_CR26","unstructured":"Arsham H. Questionnaire Design and Surveys Sampling. 2020."},{"issue":"12","key":"102_CR27","first-page":"25","volume":"5","author":"MA Zinnah","year":"2007","unstructured":"Zinnah MA, Bari MR, Islam MT, Hossain MT, Rahman MT, Haque MH, et al. CHARACTERIZATION OF ESCHERICHIA COLI ISOLATED FROM SAMPLES OF DIFFERENT BIOLOGICAL AND ENVIRONMENTAL SOURCES. Bangl J Vet Med. 2007;5(12):25\u201332.","journal-title":"Bangl J Vet Med"},{"issue":"7\u20138","key":"102_CR28","first-page":"e33","volume":"57","author":"E Seker","year":"2010","unstructured":"Seker E, Kuyucuoglu Y, Sareyyupoglu B, Yardimci H. PCR detection of Shiga toxins, enterohaemolysin and intimin virulence genes of Escherichia coli O157:H7 strains isolated from faeces of anatolian water buffaloes in Turkey. Zoonoses Public Health. 2010;57(7\u20138):e33\u201337.","journal-title":"Zoonoses Public Health"},{"key":"102_CR29","doi-asserted-by":"crossref","unstructured":"Adams MR, Moss MO. Food Microbiology. 3rd ed. Royal Society of Chemistry; 2008.","DOI":"10.1039\/9781847557940"},{"key":"102_CR30","doi-asserted-by":"publisher","first-page":"575391","DOI":"10.3389\/fmicb.2020.575391","volume":"11","author":"M Savin","year":"2020","unstructured":"Savin M, Bierbaum G, Blau K, Parcina M, Sib E, Smalla K, et al. Colistin-resistant Enterobacteriaceae isolated from process Waters and Wastewater from German Poultry and Pig slaughterhouses. Front Microbiol. 2020;11:575391.","journal-title":"Front Microbiol"},{"issue":"11","key":"102_CR31","doi-asserted-by":"publisher","first-page":"11950","DOI":"10.3390\/ijerph111111950","volume":"11","author":"LA Caine","year":"2014","unstructured":"Caine LA, Nwodo UU, Okoh AI, Ndip RN, Green E. Occurrence of virulence genes associated with diarrheagenic Escherichia coli isolated from raw cow\u2019s milk from two commercial dairy farms in the Eastern Cape Province, South Africa. Int J Environ Res Public Health. 2014;11(11):11950\u201363.","journal-title":"Int J Environ Res Public Health"},{"key":"102_CR32","doi-asserted-by":"publisher","first-page":"54","DOI":"10.3201\/eid0801.010104","volume":"8","author":"A Khan","year":"2002","unstructured":"Khan A, Yamasaki S, Sato T, Ramamurthy T, Pal A, Datta S. Prevalence and genetic profiling of virulence determinants of Non-O157 STEC isolated from cattle, beef and humans. Emerg Infect Dis. 2002;8:54\u201362.","journal-title":"Emerg Infect Dis"},{"key":"102_CR33","first-page":"83","volume":"110","author":"A Pal","year":"1999","unstructured":"Pal A, Ghosh S, Ramamurthy T, Yamasaki S, Tsukamoto T. K. BS. STEC from Healthy Cattle in a Semi Urban Community in Calcutta, India. Indian J Med Res. 1999;110:83\u20135.","journal-title":"Indian J Med Res"},{"issue":"3","key":"102_CR34","doi-asserted-by":"publisher","first-page":"3834","DOI":"10.5504\/BBEQ.2013.0031","volume":"27","author":"SA Selim","year":"2014","unstructured":"Selim SA, Ahmed SF, Aziz MHA, Zakaria AM, Klena JD, Pangallo D. Prevalence and characterization of Shiga-Toxin O157:H7 and Non-O157:H7 EnterohemorrhagicEscherichia ColiIsolated from different sources. Biotechnol Biotechnol Equip. 2014;27(3):3834\u201342.","journal-title":"Biotechnol Biotechnol Equip"},{"key":"102_CR35","doi-asserted-by":"publisher","first-page":"1375","DOI":"10.1128\/jcm.33.5.1375-1377.1995","volume":"33","author":"ST Gunzburg","year":"1995","unstructured":"Gunzburg ST, Tornieporth NG, Riley LW. Identification of EPEC by PCR based detection of the Bundle Forming Pilus Gene. J Clin Microbiol. 1995;33:1375\u20137.","journal-title":"J Clin Microbiol"},{"issue":"2","key":"102_CR36","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1016\/j.diagmicrobio.2010.01.011","volume":"67","author":"RA Nada","year":"2010","unstructured":"Nada RA, Shaheen HI, Touni I, Fahmy D, Armstrong AW, Weiner M, et al. Design and validation of a multiplex polymerase chain reaction for the identification of enterotoxigenic Escherichia coli and associated colonization factor antigens. Diagn Microbiol Infect Dis. 2010;67(2):134\u201342.","journal-title":"Diagn Microbiol Infect Dis"},{"key":"102_CR37","doi-asserted-by":"publisher","first-page":"2393","DOI":"10.1128\/jcm.32.10.2393-2397.1994","volume":"32","author":"CG Chultsz","year":"1994","unstructured":"Chultsz CG, Pool R, van Wevek KB, Speelman P. Dankert. Detection of enterotoxigenic E. Coli in stool samples by using Nonradiactively labled Oligonucleotide DNA Probs and PCR. J Clin Microbiol. 1994;32:2393\u20137.","journal-title":"J Clin Microbiol"},{"issue":"12","key":"102_CR38","doi-asserted-by":"publisher","first-page":"e0243828","DOI":"10.1371\/journal.pone.0243828","volume":"15","author":"KN Nehoya","year":"2020","unstructured":"Nehoya KN, Hamatui N, Shilangale RP, Onywera H, Kennedy J, Mwapagha LM. Characterization of Shiga toxin-producing Escherichia coli in raw beef from informal and commercial abattoirs. PLoS ONE. 2020;15(12):e0243828.","journal-title":"PLoS ONE"},{"issue":"5","key":"102_CR39","doi-asserted-by":"publisher","first-page":"407","DOI":"10.1016\/S2221-1691(13)60085-4","volume":"3","author":"M Haileselassie","year":"2013","unstructured":"Haileselassie M, Taddele H, Adhana K, Kalayou S. Food safety knowledge and practices of abattoir and butchery shops and the microbial profile of meat in Mekelle City, Ethiopia. Asian Pac J Trop Biomed. 2013;3(5):407\u201312.","journal-title":"Asian Pac J Trop Biomed"},{"issue":"8","key":"102_CR40","first-page":"213","volume":"9","author":"A Edget","year":"2017","unstructured":"Edget A, Shiferaw D, Mengistu S. Microbial safety and its public health concern of E. Coli O157:H7 and Salmonella spp. in beef at dire Dawa administrative city and Haramaya University, Ethiopia. J Veterinary Med Anim Health. 2017;9(8):213\u201327.","journal-title":"J Veterinary Med Anim Health"},{"key":"102_CR41","doi-asserted-by":"crossref","unstructured":"Bersisa A, Tulu D, Negera C. Investigation of bacteriological quality of meat from Abattoir and Butcher shops Bishoftu, Central Ethiopia. Int J Microbiol 2019:1\u20136.","DOI":"10.1155\/2019\/6416803"},{"issue":"9","key":"102_CR42","doi-asserted-by":"publisher","first-page":"789","DOI":"10.1089\/fpd.2012.1445","volume":"10","author":"AL Svoboda","year":"2013","unstructured":"Svoboda AL, Dudley EG, Debroy C, Mills EW, Cutter CN. Presence of Shiga toxin-producing Escherichia coli O-groups in small and very-small beef-processing plants and resulting ground beef detected by a multiplex polymerase chain reaction assay. Foodborne Pathog Dis. 2013;10(9):789\u201395.","journal-title":"Foodborne Pathog Dis"},{"issue":"4","key":"102_CR43","doi-asserted-by":"publisher","first-page":"649","DOI":"10.4315\/0362-028X-73.4.649","volume":"73","author":"MO MASANA","year":"2010","unstructured":"MASANA MO, LEOTTA GA, DEL CASTILLO LL, D\u2019ASTEK BA, PALLADINO PM. Prevalence, characterization, and genotypic analysis of Escherichia coli O157:H7\/NM fromSelected Beef Exporting abattoirs of Argentina. J Food Prot. 2010;73(4):649\u201356.","journal-title":"J Food Prot"}],"container-title":["One Health Outlook"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s42522-024-00102-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s42522-024-00102-y\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s42522-024-00102-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,5]],"date-time":"2024-05-05T19:04:30Z","timestamp":1714935870000},"score":1,"resource":{"primary":{"URL":"https:\/\/onehealthoutlook.biomedcentral.com\/articles\/10.1186\/s42522-024-00102-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,6]]},"references-count":43,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["102"],"URL":"https:\/\/doi.org\/10.1186\/s42522-024-00102-y","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-3616872\/v1","asserted-by":"object"}]},"ISSN":["2524-4655"],"issn-type":[{"value":"2524-4655","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,6]]},"assertion":[{"value":"14 November 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 March 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 May 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The study was approved by the Ethical Review Committee of the College of Veterinary Medicine and Animal Sciences, University of Gondar with reference number CVMAS.sc-05\/2020. Informed verbal consent and official permissions were obtained from each study participant and from the person in charge of each abattoir.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not Applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"No competing interests among authors.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"8"}}