{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T11:40:09Z","timestamp":1791632409323,"version":"4.3.4"},"reference-count":24,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2019,9,3]],"date-time":"2019-09-03T00:00:00Z","timestamp":1567468800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,9,3]],"date-time":"2019-09-03T00:00:00Z","timestamp":1567468800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"crossref","award":["FWO-SB 1S 035 16N"],"award-info":[{"award-number":["FWO-SB 1S 035 16N"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"crossref","award":["FWO-SB 1S 179 16N"],"award-info":[{"award-number":["FWO-SB 1S 179 16N"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"crossref","award":["FWO-Asp 11A0618N"],"award-info":[{"award-number":["FWO-Asp 11A0618N"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["anim microbiome"],"published-print":{"date-parts":[[2019,12]]},"DOI":"10.1186\/s42523-019-0010-6","type":"journal-article","created":{"date-parts":[[2019,9,2]],"date-time":"2019-09-02T23:05:42Z","timestamp":1567465542000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":63,"title":["The microbiome of the invertebrate model host Galleria mellonella is dominated by Enterococcus"],"prefix":"10.1186","volume":"1","author":[{"given":"Camille Nina","family":"Allonsius","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wannes","family":"Van Beeck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilke","family":"De Boeck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stijn","family":"Wittouck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Lebeer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,9,3]]},"reference":[{"issue":"3","key":"10_CR1","doi-asserted-by":"publisher","first-page":"214","DOI":"10.1080\/21505594.2015.1135289","volume":"7","author":"CJ-Y Tsai","year":"2016","unstructured":"Tsai CJ-Y, Loh JMS, Proft T. Galleria mellonella infection models for the study of bacterial diseases and for antimicrobial drug testing. Virulence. 2016;7(3):214\u201329.","journal-title":"Virulence."},{"issue":"1\u20132","key":"10_CR2","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/j.fbr.2010.04.001","volume":"24","author":"K Kavanagh","year":"2010","unstructured":"Kavanagh K, Fallon JP. Galleria mellonella larvae as models for studying fungal virulence. Fungal Biol Rev. 2010;24(1\u20132):79\u201383.","journal-title":"Fungal Biol Rev"},{"issue":"7","key":"10_CR3","doi-asserted-by":"publisher","first-page":"840","DOI":"10.1080\/21505594.2016.1203486","volume":"7","author":"OL Champion","year":"2016","unstructured":"Champion OL, Wagley S, Titball RW. Galleria mellonella as a model host for microbiological and toxin research. Virulence. 2016;7(7):840\u20135.","journal-title":"Virulence."},{"issue":"11","key":"10_CR4","doi-asserted-by":"publisher","first-page":"e1005246","DOI":"10.1371\/journal.ppat.1005246","volume":"11","author":"PR Johnston","year":"2015","unstructured":"Johnston PR, Rolff J. Host and symbiont jointly control gut microbiota during complete metamorphosis. Schneider DS, editor. PLoS Pathog. 2015;11(11):e1005246.","journal-title":"PLoS Pathog"},{"issue":"1","key":"10_CR5","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1186\/s12866-018-1377-3","volume":"18","author":"K Ignasiak","year":"2018","unstructured":"Ignasiak K, Maxwell A. Oxytetracycline reduces the diversity of tetracycline-resistance genes in the galleria mellonella gut microbiome. BMC Microbiol. 2018;18(1):228.","journal-title":"BMC Microbiol"},{"issue":"19","key":"10_CR6","doi-asserted-by":"publisher","first-page":"5824","DOI":"10.1128\/AEM.01211-16","volume":"82","author":"AC Wollenberg","year":"2016","unstructured":"Wollenberg AC, Jagdish T, Slough G, Hoinville ME, Wollenberg MS. Death becomes them: bacterial community dynamics and stilbene antibiotic production in cadavers of galleria mellonella killed by Heterorhabditis and Photorhabdus spp. Appl Environ Microbiol. 2016;82(19):5824\u201337 Elliot MA, editor.","journal-title":"Appl Environ Microbiol"},{"issue":"1","key":"10_CR7","doi-asserted-by":"publisher","first-page":"4012","DOI":"10.1038\/s41598-019-40301-6","volume":"9","author":"OV Polenogova","year":"2019","unstructured":"Polenogova OV, Kabilov MR, Tyurin MV, Rotskaya UN, Krivopalov AV, Morozova VV, et al. Parasitoid envenomation alters the galleria mellonella midgut microbiota and immunity, thereby promoting fungal infection. Sci Rep. 2019;9(1):4012.","journal-title":"Sci Rep"},{"issue":"6","key":"10_CR8","doi-asserted-by":"publisher","first-page":"e00860","DOI":"10.1128\/mBio.00860-13","volume":"4","author":"JE Blum","year":"2013","unstructured":"Blum JE, Fischer CN, Miles J, Handelsman J. Frequent replenishment sustains the beneficial microbiome of Drosophila melanogaster. MBio. 2013;4(6):e00860\u201313-e00860-13.","journal-title":"MBio."},{"issue":"5","key":"10_CR9","doi-asserted-by":"publisher","first-page":"e36765","DOI":"10.1371\/journal.pone.0036765","volume":"7","author":"EV Ridley","year":"2012","unstructured":"Ridley EV, Wong AC-N, Westmiller S, Douglas AE. Impact of the resident microbiota on the nutritional phenotype of Drosophila melanogaster. PLoS One. 2012;7(5):e36765 Leulier F, editor.","journal-title":"PLoS One"},{"issue":"2","key":"10_CR10","doi-asserted-by":"publisher","first-page":"374","DOI":"10.1016\/j.ydbio.2006.05.006","volume":"297","author":"JM Bates","year":"2006","unstructured":"Bates JM, Mittge E, Kuhlman J, Baden KN, Cheesman SE, Guillemin K. Distinct signals from the microbiota promote different aspects of zebrafish gut differentiation. Dev Biol. 2006;297(2):374\u201386.","journal-title":"Dev Biol"},{"issue":"1","key":"10_CR11","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1186\/s40168-015-0083-8","volume":"3","author":"J Jervis-Bardy","year":"2015","unstructured":"Jervis-Bardy J, Leong LEX, Marri S, Smith RJ, Choo JM, Smith-Vaughan HC, et al. Deriving accurate microbiota profiles from human samples with low bacterial content through post-sequencing processing of Illumina MiSeq data. Microbiome. 2015;3(1):19.","journal-title":"Microbiome."},{"issue":"1","key":"10_CR12","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1186\/s12915-014-0087-z","volume":"12","author":"SJ Salter","year":"2014","unstructured":"Salter SJ, Cox MJ, Turek EM, Calus ST, Cookson WO, Moffatt MF, et al. Reagent and laboratory contamination can critically impact sequence-based microbiome analyses. BMC Biol. 2014;12(1):87.","journal-title":"BMC Biol"},{"issue":"5","key":"10_CR13","doi-asserted-by":"publisher","first-page":"407","DOI":"10.1016\/0022-1910(83)90068-9","volume":"29","author":"NA Ratcliffe","year":"1983","unstructured":"Ratcliffe NA, Walters JB. Studies on the in vivo cellular reactions of insects: clearance of pathogenic and non-pathogenic bacteria in galleria mellonella larvae. J Insect Physiol. 1983;29(5):407\u201315.","journal-title":"J Insect Physiol"},{"issue":"Pt 3","key":"10_CR14","doi-asserted-by":"publisher","first-page":"716","DOI":"10.1099\/ijs.0.038075-0","volume":"62","author":"O-S Kim","year":"2012","unstructured":"Kim O-S, Cho Y-J, Lee K, Yoon S-H, Kim M, Na H, et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol. 2012;62(Pt 3):716\u201321.","journal-title":"Int J Syst Evol Microbiol"},{"key":"10_CR15","unstructured":"Lebreton F, Willems RJL, Gilmore MS. Enterococcus diversity, origins in nature, and gut colonization [internet]. Enterococci: from commensals to leading causes of drug resistant infection. Massachusetts Eye and Ear Infirmary; 2014. pmid: 24649513."},{"key":"10_CR16","doi-asserted-by":"crossref","unstructured":"Milani C, Duranti S, Bottacini F, Casey E, Turroni F, Mahony J, et al. The first microbial colonizers of the human gut: composition, activities, and health implications of the infant gut microbiota. Microbiol Mol Biol Rev. 2017;81(4).","DOI":"10.1128\/MMBR.00036-17"},{"issue":"2","key":"10_CR17","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1111\/j.1365-2672.2010.04675.x","volume":"109","author":"BA Layton","year":"2010","unstructured":"Layton BA, Walters SP, Lam LH, Boehm AB. Enterococcus species distribution among human and animal hosts using multiplex PCR. J Appl Microbiol. 2010;109(2):539\u201347.","journal-title":"J Appl Microbiol"},{"issue":"7","key":"10_CR18","doi-asserted-by":"publisher","first-page":"5702","DOI":"10.3168\/jds.2017-14151","volume":"101","author":"K Kim","year":"2018","unstructured":"Kim K, Lee G, Thanh HD, Kim J-H, Konkit M, Yoon S, et al. Exopolysaccharide from Lactobacillus plantarum LRCC5310 offers protection against rotavirus-induced diarrhea and regulates inflammatory response. J Dairy Sci. 2018;101(7):5702\u201312.","journal-title":"J Dairy Sci"},{"issue":"12","key":"10_CR19","doi-asserted-by":"crossref","first-page":"fiy186","DOI":"10.1093\/femsec\/fiy186","volume":"94","author":"F Van Herreweghen","year":"2018","unstructured":"Van Herreweghen F, De Paepe K, Roume H, Kerckhof F-M, Van de Wiele T. Mucin degradation niche as a driver of microbiome composition and Akkermansia muciniphila abundance in a dynamic gut model is donor independent. FEMS Microbiol Ecol. 2018;94(12):fiy186 pmid: 30239657.","journal-title":"FEMS Microbiol Ecol"},{"issue":"Supplement_1","key":"10_CR20","doi-asserted-by":"publisher","first-page":"4516","DOI":"10.1073\/pnas.1000080107","volume":"108","author":"J. G. Caporaso","year":"2010","unstructured":"Caporaso JG, Lauber CL, Walters WA, Berg-lyons D, Lozupone CA, Turnbaugh PJ, et al. Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample. Proc Natl Acad Sci U S A. 2010;108(1).","journal-title":"Proceedings of the National Academy of Sciences"},{"issue":"17","key":"10_CR21","doi-asserted-by":"publisher","first-page":"5112","DOI":"10.1128\/AEM.01043-13","volume":"79","author":"JJ Kozich","year":"2013","unstructured":"Kozich JJ, Westcott SL, Baxter NT, Highlander SK, Schloss PD. Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform. Appl Environ Microbiol. 2013;79(17):5112\u201320 pmid: 23793624.","journal-title":"Appl Environ Microbiol"},{"issue":"7","key":"10_CR22","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1038\/nmeth.3869","volume":"13","author":"BJ Callahan","year":"2016","unstructured":"Callahan BJ, Mcmurdie PJ, Rosen MJ, Han AW, Johnson AJ, Holmes SP. DADA2: high-resolution sample inference from Illumina amplicon data. Nat Methods. 2016;13(7):581 pmid: 27214047.","journal-title":"Nat Methods"},{"issue":"1","key":"10_CR23","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1111\/j.1467-985X.2010.00676_9.x","volume":"174","author":"C Ginestet","year":"2011","unstructured":"Ginestet C. ggplot2: elegant graphics for data analysis. J R Stat Soc Ser A (Statistics Soc). 2011;174(1):245\u20136 pmid: 387981403.","journal-title":"J R Stat Soc Ser A (Statistics Soc)"},{"key":"10_CR24","volume-title":"Vegan: community ecology package. R Packag 23\u20133","author":"J Oksanen","year":"2016","unstructured":"Oksanen J, Blanchet F, Kindt R, Legendre P, O\u2019Hara R. Vegan: community ecology package. R Packag 23\u20133. 2016; pmid: 17666792."}],"container-title":["Animal Microbiome"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s42523-019-0010-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/s42523-019-0010-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s42523-019-0010-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,27]],"date-time":"2022-09-27T05:45:41Z","timestamp":1664257541000},"score":1,"resource":{"primary":{"URL":"https:\/\/animalmicrobiome.biomedcentral.com\/articles\/10.1186\/s42523-019-0010-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,3]]},"references-count":24,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["10"],"URL":"https:\/\/doi.org\/10.1186\/s42523-019-0010-6","relation":{},"ISSN":["2524-4671"],"issn-type":[{"value":"2524-4671","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,3]]},"assertion":[{"value":"16 May 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 July 2019","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 September 2019","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Not applicable.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"7"}}