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Although FCoV generally causes mild symptoms, approximately 5% of cases progress to death in cats worldwide. FCoV shares certain virological features with severe acute respiratory syndrome coronavirus 2 that causes COVID-19, indicating that common therapeutic strategies may be applicable. GS-441524 the parent drug of remdesivir and a competitive inhibitor of nucleoside triphosphates in viral RNA synthesis is a well-known treatment for FIP. However, comparative transcriptome and gene ontology analyses of normal (Normal), FIP-diseased (FIPD), and FIP-recovered (FIPR) cats have not yet been conducted.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>In this study, we compared the mRNA expression profiles of peripheral blood mononuclear cells from Normal, FIPD, and FIPR cats to identify immunological alterations. We identified 677 (FIPD\/Normal) and 431 (FIPR\/FIPD) differentially expressed genes with statistical significance. These data were input into the bioinformatics program. As a result, the analysis revealed statistically significant and contrasting patterns of canonical pathways of neutrophil degranulation and interleukin-8 (IL-8) signaling pathways. Additionally, we observed that kruppel-like factor 6 (KLF6) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-\u03baB) were upstream molecules of IL-8, promoting neutrophil activation and function.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>This study identified immunological alterations in PBMCs of Normal, FIPD, and FIPR cats. KLF-6 and NF-\u03baB were found to regulate IL-8-mediated neutrophil activation.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/s42826-025-00247-5","type":"journal-article","created":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T09:17:25Z","timestamp":1749806245000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative transcriptome analysis of PBMCs in cats diagnosed with and recovered from FIPV"],"prefix":"10.1186","volume":"41","author":[{"given":"Ju Young","family":"Lee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyeong Ryeol","family":"Cho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong-Geun","family":"Oh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4763-8373","authenticated-orcid":false,"given":"Jeong Ho","family":"Hwang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,6,13]]},"reference":[{"issue":"1","key":"247_CR1","doi-asserted-by":"publisher","first-page":"150","DOI":"10.1006\/viro.1998.9045","volume":"243","author":"H Vennema","year":"1998","unstructured":"Vennema H, Poland A, Foley J, Pedersen NC. 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