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Despite the advancement in treatment, drug resistance remains a major cause for setback. In an earlier work, the authors reported that<jats:italic>Boswellia dalzielii<\/jats:italic>(Hutch) stem bark exhibited dose-dependent cytotoxicity in head and neck cancer cells, AW8507. Therefore, the cell death induction effect of<jats:italic>Boswellia dalzielii<\/jats:italic>stem bark chloroform extract in head and neck cancer cell line, AW8507, and its derived constituent on cell cycle and apoptosis proteins was further investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The cell death induction activity of the<jats:italic>Boswellia dalzielii<\/jats:italic>stem bark chloroform fraction (CLBD) in AW8507 was determined using Annexin V-FITC\/PI staining in flow cytometry. High-performance liquid chromatography-mass spectrometry was employed for compounds analysis of the CLBD, and reverse virtual screening was used to identify the mechanism of action of the compound, acetyl-11-keto-beta-boswellic acid, that was elucidated in the<jats:italic>Boswellia dalzielii<\/jats:italic>chloroform fraction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The data obtained showed that<jats:italic>Boswellia dalzielii<\/jats:italic>stem bark Chloroform extract increased the percentage of cells presenting for early apoptosis from 4.14 to 10.10% in AW8507 cells. High-performance liquid chromatography-mass spectrometry analysis of the chloroform fraction identified acetyl-11-keto-beta-boswellic acid. Reverse virtual screening on selected proteins showed that acetyl-11-keto-beta-boswellic acid is a multi-protein target compound. It binds preferably to phosphorylated-cyclin dependent kinase 1 (p-CDK1) (binding score\u2009=\u2009\u2009\u2212\u20099.2\u00a0kcal\/mol), blocking the activation of cyclin B-CDK1 needed for cell cycle progression at G2\/M phase of the cell cycle. Acetyl-11-keto-beta-boswellic acid also binds more tightly with \u03b1\u03b2 tubulin (binding score\u2009=\u20098.9\u00a0kcal\/mol) than with the standard drug, docetaxel (binding score\u2009=\u20098.3\u00a0kcal\/mol).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The results obtained confirmed the culpability of<jats:italic>Boswellia dalzielii<\/jats:italic>-derived acetyl-11-keto-beta-boswellic acid in the obstruction of the cell cycle progression in head and neck cancer cell line, AW8507; and the induction of apoptosis earlier reported for<jats:italic>Boswellia dalzielii<\/jats:italic>(Hutch) stem bark. Additional in vitro and\/or in vivo studies would be required to validate in silico observations.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s43046-021-00075-3","type":"journal-article","created":{"date-parts":[[2021,8,8]],"date-time":"2021-08-08T23:04:37Z","timestamp":1628463877000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Chloroform extract and acetyl-11-keto-beta-boswellic acid from Boswellia dalzielii stem bark induce apoptosis and cell cycle blockage in AW8507 cells"],"prefix":"10.1186","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3226-1534","authenticated-orcid":false,"given":"Akinbobola Peace","family":"Otitoju","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ishaya Yohanna","family":"Longdet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taiwo Emmanuel","family":"Alemika","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vikram Prakash","family":"Gota","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,8,9]]},"reference":[{"key":"75_CR1","doi-asserted-by":"publisher","first-page":"359","DOI":"10.1002\/ijc.29210","volume":"136","author":"J Ferlay","year":"2015","unstructured":"Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, et al. 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