{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:40:50Z","timestamp":1790689250802,"version":"4.1.0"},"reference-count":34,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"National Key Research and Development Program of China","award":["2022YFD1200502"],"award-info":[{"award-number":["2022YFD1200502"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Horticulture"],"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:p>Terpenoids, a group of metabolites, are important to plant development and color formation, and serve as valuable nutrients for humans. The enzyme 4-diphosphocytidyl- 2\u2009C-methyl-D-erythritol cytidyltransferase (MCT) plays a pivotal role in the methylerythritol phosphate (MEP) pathway for terpenoid biosynthesis. However, the potential lethality of <jats:italic>MCT<\/jats:italic> mutants has hindered further exploration into its functional role in terpenoid metabolite families in plants. Here, we characterized a rare <jats:italic>MCT<\/jats:italic> mutant <jats:italic>yfm<\/jats:italic> with dwarfism, chlorosis, small leaves, and yellow fruits in tomato. Map-based cloning and sequence analysis revealed that a single nucleotide substitution in the <jats:italic>SlMCT<\/jats:italic> gene, which resulted in a point mutation (Leu297Pro) in amino acid in the mutant. Over-expression and complementation of the wild-type <jats:italic>SlMCT<\/jats:italic>\n            <jats:sup>\n              <jats:italic>T<\/jats:italic>\n            <\/jats:sup> in the <jats:italic>yfm<\/jats:italic> mutant restored the fruit color and the other defective phenotypes. This mutation altered the gene expressions and metabolic components in the MEP and other pathways. Consequently, the total contents of carotenoids, chlorophyll, IAA, GAs, and SA were decreased, while the contents of CK, JA, and ABA were increased. Eventually, these alterations led to changes in plant phenotypes and fruit color in <jats:italic>yfm<\/jats:italic>. These findings provide novel insights into understanding the roles of MCT on plant development and pigment biosynthesis.<\/jats:p>","DOI":"10.1186\/s43897-025-00159-x","type":"journal-article","created":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T23:02:44Z","timestamp":1754002964000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A single nucleotide substitution in the SlMCT gene contributes to great morphological alternations in tomato"],"prefix":"10.1186","volume":"5","author":[{"given":"Mengyi","family":"Yu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinge","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zilin","family":"Qian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huolin","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5369-2555","authenticated-orcid":false,"given":"Wencai","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,8,1]]},"reference":[{"issue":"1","key":"159_CR1","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1186\/s13059-021-02283-5","volume":"22","author":"V Akbari","year":"2021","unstructured":"Akbari V, Garant JM, O\u2019Neill K, Pandoh P, Moore R, et al. Megabase-scale methylation phasing using nanopore long reads and NanoMethPhase. Genome Biol. 2021;22(1):68. https:\/\/doi.org\/10.1186\/s13059-021-02283-5.","journal-title":"Genome Biol"},{"issue":"11","key":"159_CR2","doi-asserted-by":"publisher","DOI":"10.1080\/15592324.2017.1387708","volume":"12","author":"P Andrade","year":"2017","unstructured":"Andrade P, Caudep\u00f3n D, Altabella T, Arr\u00f3 M, Ferrer A, Manzano D. Complex interplays between phytosterols and plastid development. Plant Signal Behav. 2017;12(11): e1387708. https:\/\/doi.org\/10.1080\/15592324.2017.1387708.","journal-title":"Plant Signal Behav"},{"key":"159_CR3","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1016\/j.ymben.2023.03.002","volume":"77","author":"J Chen","year":"2023","unstructured":"Chen J, Tan J, Duan X, Wang Y, Wen J, et al. Plastidial engineering with coupled farnesyl diphosphate pool reconstitution and enhancement for sesquiterpene biosynthesis in tomato fruit. Metab Eng. 2023;77:41\u201352. https:\/\/doi.org\/10.1016\/j.ymben.2023.03.002.","journal-title":"Metab Eng"},{"issue":"5","key":"159_CR4","doi-asserted-by":"publisher","first-page":"572","DOI":"10.1016\/j.tplants.2024.02.007","volume":"29","author":"W Contreras-Avil\u00e9s","year":"2024","unstructured":"Contreras-Avil\u00e9s W, Heuvelink E, Marcelis LFM, Kappers IF. M\u00e9nage \u00e0 trois: light, terpenoids, and quality of plants. Trends Plant Sci. 2024;29(5):572\u201388. https:\/\/doi.org\/10.1016\/j.tplants.2024.02.007.","journal-title":"Trends Plant Sci"},{"issue":"6","key":"159_CR5","doi-asserted-by":"publisher","first-page":"2023","DOI":"10.1093\/jxb\/erq393","volume":"62","author":"E Cordoba","year":"2011","unstructured":"Cordoba E, Porta H, Arroyo A, San Rom\u00e1n C, Medina L, et al. Functional characterization of the three genes encoding 1-deoxy-D-xylulose 5-phosphate synthase in maize. 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