{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T09:20:33Z","timestamp":1790328033436,"version":"4.1.0"},"reference-count":30,"publisher":"Springer Science and Business Media LLC","issue":"S1","license":[{"start":{"date-parts":[[2013,12,1]],"date-time":"2013-12-01T00:00:00Z","timestamp":1385856000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2013,12,1]],"date-time":"2013-12-01T00:00:00Z","timestamp":1385856000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Stem Cell Res Ther"],"published-print":{"date-parts":[[2013,12]]},"DOI":"10.1186\/scrt379","type":"journal-article","created":{"date-parts":[[2013,12,20]],"date-time":"2013-12-20T16:04:07Z","timestamp":1387555447000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":62,"title":["Neurovascular unit on a chip: implications for translational applications"],"prefix":"10.1186","volume":"4","author":[{"given":"Donald J","family":"Alcendor","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank E","family":"Block III","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David E","family":"Cliffel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John Scott","family":"Daniels","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kate LJ","family":"Ellacott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cody R","family":"Goodwin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas H","family":"Hofmeister","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deyu","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitry A","family":"Markov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jody C","family":"May","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa J","family":"McCawley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"BethAnn","family":"McLaughlin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John A","family":"McLean","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin D","family":"Niswender","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginia","family":"Pensabene","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin T","family":"Seale","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stacy D","family":"Sherrod","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hak-Joon","family":"Sung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David L","family":"Tabb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donna J","family":"Webb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John P","family":"Wikswo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2013,12,20]]},"reference":[{"key":"293_CR1","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1146\/annurev-bioeng-071910-124629","volume":"13","author":"MB Esch","year":"2011","unstructured":"Esch MB, King TL, Shuler ML: The role of body-on-a-chip devices in drug and toxicity studies. Annu Rev Biomed Engr. 2011, 13: 55-72. 10.1146\/annurev-bioeng-071910-124629.","journal-title":"Annu Rev Biomed Engr"},{"key":"293_CR2","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1039\/c2ib00176d","volume":"4","author":"AD van der Meer","year":"2012","unstructured":"van der Meer AD, van den Berg A: Organs-on-chips: breaking the in vitro impasse. Integr Biol. 2012, 4: 461-470. 10.1039\/c2ib00176d.","journal-title":"Integr Biol"},{"key":"293_CR3","doi-asserted-by":"publisher","first-page":"2156","DOI":"10.1039\/c2lc40089h","volume":"12","author":"D Huh","year":"2012","unstructured":"Huh D, Torisawa YS, Hamilton GA, Kim HJ, Ingber DE: Microengineered physiological biomimicry: organs-on-chips. Lab Chip. 2012, 12: 2156-2164. 10.1039\/c2lc40089h.","journal-title":"Lab Chip"},{"key":"293_CR4","doi-asserted-by":"publisher","first-page":"682","DOI":"10.1109\/TBME.2013.2244891","volume":"60","author":"JP Wikswo","year":"2013","unstructured":"Wikswo JP, Block FE, Cliffel DE, Goodwin CR, Marasco CC, Markov DA, McLean DL, McLean JA, McKenzie JR, Reiserer RS, Samson PC, Schaffer DK, Seale KT, Sherrod SD: Engineering challenges for instrumenting and controlling integrated organ-on-a-chip systems. IEEE Trans Biomed Eng. 2013, 60: 682-690.","journal-title":"IEEE Trans Biomed Eng"},{"key":"293_CR5","unstructured":"Integrated Microphysiological Systems for Drug Efficacy and Toxicity Testing in Human Health and Disease (UH2\/UH3). [http:\/\/grants.nih.gov\/grants\/guide\/rfa-files\/RFA-RM-11-022.html]"},{"key":"293_CR6","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1124\/jpet.107.130294","volume":"325","author":"XR Liu","year":"2008","unstructured":"Liu XR, Chen CP, Smith BJ: Progress in brain penetration evaluation in drug discovery and development. J Pharmacol Exp Ther. 2008, 325: 349-356. 10.1124\/jpet.107.130294.","journal-title":"J Pharmacol Exp Ther"},{"key":"293_CR7","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1007\/s10544-012-9699-7","volume":"15","author":"LM Griep","year":"2013","unstructured":"Griep LM, Wolbers F, de Wagenaar B, ter Braak PM, Weksler BB, Romero IA, Couraud PO, Vermes I, van der Meer AD, van den Berg A: BBB ON CHIP: microfluidic platform to mechanically and biochemically modulate blood-brain barrier function. Biomed Microdevices. 2013, 15: 145-150. 10.1007\/s10544-012-9699-7.","journal-title":"Biomed Microdevices"},{"key":"293_CR8","doi-asserted-by":"publisher","first-page":"1093","DOI":"10.1039\/c2lc41208j","volume":"13","author":"B Prabhakarpandian","year":"2013","unstructured":"Prabhakarpandian B, Shen MC, Nichols JB, Mills IR, Sidoryk-Wegrzynowicz M, Aschner M, Pant K: SyM-BBB: a microfluidic blood brain barrier model. Lab Chip. 2013, 13: 1093-1101. 10.1039\/c2lc41208j.","journal-title":"Lab Chip"},{"key":"293_CR9","doi-asserted-by":"publisher","first-page":"1784","DOI":"10.1039\/c2lc40094d","volume":"12","author":"R Booth","year":"2012","unstructured":"Booth R, Kim H: Characterization of a microfluidic in vitro model of the blood-brain barrier (\u03bcBBB). Lab Chip. 2012, 12: 1784-1792. 10.1039\/c2lc40094d.","journal-title":"Lab Chip"},{"key":"293_CR10","doi-asserted-by":"publisher","first-page":"542","DOI":"10.1039\/C2LC41033H","volume":"13","author":"AKH Achyuta","year":"2013","unstructured":"Achyuta AKH, Conway AJ, Crouse RB, Bannister EC, Lee RN, Katnik CP, Behensky AA, Cuevas J, Sundaram SS: A modular approach to create a neurovascular unit-on-a-chip. Lab Chip. 2013, 13: 542-553. 10.1039\/c2lc41033h.","journal-title":"Lab Chip"},{"key":"293_CR11","doi-asserted-by":"publisher","first-page":"767","DOI":"10.1038\/jcbfm.2010.162","volume":"31","author":"L Cucullo","year":"2011","unstructured":"Cucullo L, Marchi N, Hossain M, Janigro D: A dynamic in vitro BBB model for the study of immune cell trafficking into the central nervous system. J Cereb Blood Flow Metab. 2011, 31: 767-777. 10.1038\/jcbfm.2010.162.","journal-title":"J Cereb Blood Flow Metab"},{"key":"293_CR12","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1007\/s10544-011-9523-9","volume":"13","author":"Y Gao","year":"2011","unstructured":"Gao Y, Majumdar D, Jovanovic B, Shaifer C, Lin P, Zijlstra A, Webb D, Li D: A versatile valve-enabled microfluidic cell co-culture platform and demonstration of its applications to neurobiology and cancer biology. Biomed Microdevices. 2011, 13: 539-548. 10.1007\/s10544-011-9523-9.","journal-title":"Biomed Microdevices"},{"key":"293_CR13","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1146\/annurev-bioeng-071910-124651","volume":"13","author":"PR LeDuc","year":"2011","unstructured":"LeDuc PR, Messner WC, Wikswo JP: How do control-based approaches enter into biology?. Annu Rev Biomed Engr. 2011, 13: 369-396. 10.1146\/annurev-bioeng-071910-124651.","journal-title":"Annu Rev Biomed Engr"},{"key":"293_CR14","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1186\/1742-2094-9-95","volume":"9","author":"DJ Alcendor","year":"2012","unstructured":"Alcendor DJ, Charest AM, Zhu WQ, Vigil HE, Knobel SM: Infection and upregulation of proinflammatory cytokines in human brain vascular pericytes by human cytomegalovirus. J Neuroinflamm. 2012, 9: 95-10.1186\/1742-2094-9-95.","journal-title":"J Neuroinflamm"},{"key":"293_CR15","doi-asserted-by":"publisher","first-page":"562","DOI":"10.1038\/nature09513","volume":"468","author":"R Daneman","year":"2010","unstructured":"Daneman R, Zhou L, Kebede AA, Barres BA: Pericytes are required for blood-brain barrier integrity during embryogenesis. Nature. 2010, 468: 562-566. 10.1038\/nature09513.","journal-title":"Nature"},{"key":"293_CR16","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1038\/35067088","volume":"2","author":"S Tsukita","year":"2001","unstructured":"Tsukita S, Furuse M, Itoh M: Multifunctional strands in tight junctions. Nat Rev Mol Cell Biol. 2001, 2: 285-293. 10.1038\/35067088.","journal-title":"Nat Rev Mol Cell Biol"},{"key":"293_CR17","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1096\/fj.01-0814fje","volume":"16","author":"M Ramsauer","year":"2002","unstructured":"Ramsauer M, Krause D, Dermietzel R: Angiogenesis of the blood-brain barrier in vitro and the function of cerebral pericytes. FASEB J. 2002, 16: 1274-1276.","journal-title":"FASEB J"},{"key":"293_CR18","doi-asserted-by":"publisher","first-page":"258","DOI":"10.1016\/j.brainresrev.2005.07.004","volume":"50","author":"CH Lai","year":"2005","unstructured":"Lai CH, Kuo KH: The critical component to establish in vitro BBB model: pericyte. Brain Res Rev. 2005, 50: 258-265. 10.1016\/j.brainresrev.2005.07.004.","journal-title":"Brain Res Rev"},{"key":"293_CR19","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1007\/s00441-003-0751-z","volume":"314","author":"B Engelhardt","year":"2003","unstructured":"Engelhardt B: Development of the blood-brain barrier. Cell Tissue Res. 2003, 314: 119-129. 10.1007\/s00441-003-0751-z.","journal-title":"Cell Tissue Res"},{"key":"293_CR20","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1016\/j.tiv.2004.06.011","volume":"19","author":"P Garberg","year":"2005","unstructured":"Garberg P, Ball M, Borg N, Cecchelli R, Fenart L, Hurst RD, Lindmark T, Mabondzo A, Nilsson JE, Raub TJ, Stanimirovic D, Terasaki T, Oberg JO, Osterberg T: In vitro models for the blood-brain barrier. Toxicol In Vitro. 2005, 19: 299-334. 10.1016\/j.tiv.2004.06.011.","journal-title":"Toxicol In Vitro"},{"key":"293_CR21","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1186\/1471-2202-12-40","volume":"12","author":"L Cucullo","year":"2011","unstructured":"Cucullo L, Hossain M, Puvenna V, Marchi N, Janigro D: The role of shear stress in blood-brain barrier endothelial physiology. BMC Neurosci. 2011, 12: 40-10.1186\/1471-2202-12-40.","journal-title":"BMC Neurosci"},{"key":"293_CR22","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.brainres.2006.06.027","volume":"1109","author":"S Santaguida","year":"2006","unstructured":"Santaguida S, Janigro D, Hossain M, Oby E, Rapp E, Cucullo L: Side by side comparison between dynamic versus static models of blood-brain barrier in vitro: a permeability study. Brain Res. 2006, 1109: 1-13. 10.1016\/j.brainres.2006.06.027.","journal-title":"Brain Res"},{"key":"293_CR23","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1096\/fasebj.7.11.8370472","volume":"7","author":"D Shepro","year":"1993","unstructured":"Shepro D, Morel NML: Pericyte physiology. FASEB J. 1993, 7: 1031-1038.","journal-title":"FASEB J"},{"key":"293_CR24","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1016\/S0006-8993(02)03167-0","volume":"951","author":"L Cucullo","year":"2002","unstructured":"Cucullo L, McAllister MS, Kight K, Krizanac-Bengez L, Marroni M, Mayberg MR, Stanness KA, Janigro D: A new dynamic in vitro model for the multidimensional study of astrocyte-endothelial cell interactions at the blood-brain barrier. Brain Res. 2002, 951: 243-254. 10.1016\/S0006-8993(02)03167-0.","journal-title":"Brain Res"},{"key":"293_CR25","doi-asserted-by":"publisher","first-page":"159ra147","DOI":"10.1126\/scitranslmed.3004249","volume":"4","author":"D Huh","year":"2012","unstructured":"Huh D, Leslie DC, Matthews BD, Fraser JP, Jurek S, Hamilton GA, Thorneloe KS, McAlexander MA, Ingber DE: A human disease model of drug toxicity-induced pulmonary edema in a lung-on-a-chip microdevice. Sci Transl Med. 2012, 4: 159ra147-","journal-title":"Sci Transl Med"},{"key":"293_CR26","doi-asserted-by":"publisher","first-page":"2165","DOI":"10.1039\/c2lc40074j","volume":"12","author":"HJ Kim","year":"2012","unstructured":"Kim HJ, Huh D, Hamilton G, Ingber DE: Human gut-on-a-chip inhabited by microbial flora that experiences intestinal peristalsis-like motions and flow. Lab Chip. 2012, 12: 2165-2174. 10.1039\/c2lc40074j.","journal-title":"Lab Chip"},{"key":"293_CR27","doi-asserted-by":"publisher","first-page":"1662","DOI":"10.1126\/science.1188302","volume":"328","author":"D Huh","year":"2010","unstructured":"Huh D, Matthews BD, Mammoto A, Montoya-Zavala M, Hsin HY, Ingber DE: Reconstituting organ-level lung functions on a chip. Science. 2010, 328: 1662-1668. 10.1126\/science.1188302.","journal-title":"Science"},{"key":"293_CR28","doi-asserted-by":"publisher","first-page":"505","DOI":"10.1111\/j.1528-1167.2006.00960.x","volume":"48","author":"L Cucullo","year":"2007","unstructured":"Cucullo L, Hossain M, Rapp E, Manders T, Marchi N, Janigro D: Development of a humanized in vitro blood-brain barrier model to screen for brain penetration of antiepileptic drugs. Epilepsia. 2007, 48: 505-516. 10.1111\/j.1528-1167.2006.00960.x.","journal-title":"Epilepsia"},{"key":"293_CR29","doi-asserted-by":"publisher","first-page":"562","DOI":"10.1111\/j.1528-1167.2010.02956.x","volume":"52","author":"C Ghosh","year":"2011","unstructured":"Ghosh C, Marchi N, Desai NK, Puvenna V, Hossain M, Gonzalez-Martinez J, Alexopoulos AV, Janigro D: Cellular localization and functional significance of CYP3A4 in the human epileptic brain. Epilepsia. 2011, 52: 562-571. 10.1111\/j.1528-1167.2010.02956.x.","journal-title":"Epilepsia"},{"key":"293_CR30","doi-asserted-by":"publisher","first-page":"e56805","DOI":"10.1371\/journal.pone.0056805","volume":"8","author":"N Marchi","year":"2013","unstructured":"Marchi N, Bazarian JJ, Puvenna V, Janigro M, Ghosh C, Zhong J, Zhu T, Blackman E, Stewart D, Ellis J, Butler R, Janigro D: Consequences of repeated blood-brain barrier disruption in football players. PloS One. 2013, 8: e56805-10.1371\/journal.pone.0056805.","journal-title":"PloS One"}],"container-title":["Stem Cell Research & Therapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/scrt379.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/scrt379\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/scrt379.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T06:12:37Z","timestamp":1637043157000},"score":1,"resource":{"primary":{"URL":"https:\/\/stemcellres.biomedcentral.com\/articles\/10.1186\/scrt379"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,12]]},"references-count":30,"journal-issue":{"issue":"S1","published-print":{"date-parts":[[2013,12]]}},"alternative-id":["293"],"URL":"https:\/\/doi.org\/10.1186\/scrt379","relation":{},"ISSN":["1757-6512"],"issn-type":[{"value":"1757-6512","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,12]]},"assertion":[{"value":"20 December 2013","order":1,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}],"article-number":"S18"}}