{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T13:19:06Z","timestamp":1761743946906},"reference-count":26,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2014,6,11]],"date-time":"2014-06-11T00:00:00Z","timestamp":1402444800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/2.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Bioinformatics"],"published-print":{"date-parts":[[2014,12]]},"DOI":"10.1186\/1471-2105-15-181","type":"journal-article","created":{"date-parts":[[2014,6,11]],"date-time":"2014-06-11T00:41:46Z","timestamp":1402447306000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Spot quantification in two dimensional gel electrophoresis image analysis: comparison of different approaches and presentation of a novel compound fitting algorithm"],"prefix":"10.1186","volume":"15","author":[{"given":"Jan M","family":"Brauner","sequence":"first","affiliation":[]},{"given":"Teja W","family":"Groemer","sequence":"additional","affiliation":[]},{"given":"Armin","family":"Stroebel","sequence":"additional","affiliation":[]},{"given":"Simon","family":"Grosse-Holz","sequence":"additional","affiliation":[]},{"given":"Timo","family":"Oberstein","sequence":"additional","affiliation":[]},{"given":"Jens","family":"Wiltfang","sequence":"additional","affiliation":[]},{"given":"Johannes","family":"Kornhuber","sequence":"additional","affiliation":[]},{"given":"Juan Manuel","family":"Maler","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2014,6,11]]},"reference":[{"key":"6475_CR1","doi-asserted-by":"publisher","first-page":"896718","DOI":"10.1155\/2010\/896718","volume":"2010","author":"KF Sellers","year":"2010","unstructured":"Sellers KF, Miecznikowski JC: Feature detection techniques for preprocessing proteomic data. Int J Biomed Imaging. 2010, 2010: 896718-","journal-title":"Int J Biomed Imaging"},{"key":"6475_CR2","doi-asserted-by":"publisher","first-page":"706","DOI":"10.1002\/elps.200700244","volume":"29","author":"T Srinark","year":"2008","unstructured":"Srinark T, Kambhamettu C: An image analysis suite for spot detection and spot matching in two-dimensional electrophoresis gels. Electrophoresis. 2008, 29: 706-715. 10.1002\/elps.200700244.","journal-title":"Electrophoresis"},{"key":"6475_CR3","doi-asserted-by":"publisher","first-page":"595","DOI":"10.1016\/B978-0-12-381270-4.00021-4","volume":"487","author":"F Li","year":"2011","unstructured":"Li F, Seillier-Moiseiwitsch F: Differential analysis of 2D gel images. Methods Enzymol. 2011, 487: 595-609.","journal-title":"Methods Enzymol"},{"key":"6475_CR4","doi-asserted-by":"publisher","first-page":"4914","DOI":"10.1002\/pmic.200800644","volume":"8","author":"O Langella","year":"2008","unstructured":"Langella O, Zivy M: A method based on bead flows for spot detection on 2-D gel images. Proteomics. 2008, 8: 4914-4918. 10.1002\/pmic.200800644.","journal-title":"Proteomics"},{"key":"6475_CR5","doi-asserted-by":"publisher","first-page":"1115","DOI":"10.1002\/jemt.20858","volume":"73","author":"A Stroebel","year":"2010","unstructured":"Stroebel A, Welzel O, Kornhuber J, Groemer TW: Background determination-based detection of scattered peaks. Microsc Res Tech. 2010, 73: 1115-1122. 10.1002\/jemt.20858.","journal-title":"Microsc Res Tech"},{"key":"6475_CR6","first-page":"437","volume":"37","author":"M Natale","year":"2011","unstructured":"Natale M, Maresca B, Abrescia P, Bucci EM: Image analysis workflow for 2-D electrophoresis gels based on ImageJ. PRI. 2011, 37: 437-449.","journal-title":"PRI"},{"key":"6475_CR7","doi-asserted-by":"publisher","first-page":"368","DOI":"10.1093\/bioinformatics\/btq666","volume":"27","author":"A dos Anjos","year":"2011","unstructured":"dos Anjos A, Moller AL, Ersboll BK, Finnie C, Shahbazkia HR: New approach for segmentation and quantification of two-dimensional gel electrophoresis images. Bioinformatics. 2011, 27: 368-375. 10.1093\/bioinformatics\/btq666.","journal-title":"Bioinformatics"},{"key":"6475_CR8","doi-asserted-by":"publisher","first-page":"529","DOI":"10.1093\/bioinformatics\/btm590","volume":"24","author":"JS Morris","year":"2008","unstructured":"Morris JS, Clark BN, Gutstein HB: Pinnacle: a fast, automatic and accurate method for detecting and quantifying protein spots in 2-dimensional gel electrophoresis data. Bioinformatics. 2008, 24: 529-536. 10.1093\/bioinformatics\/btm590.","journal-title":"Bioinformatics"},{"issue":"9","key":"6475_CR9","doi-asserted-by":"crossref","first-page":"5269","DOI":"10.1016\/S0021-9258(18)83728-0","volume":"64","author":"JI Garrels","year":"1989","unstructured":"Garrels JI: The QUEST system for quantitative analysis of two-dimensional gels. J Biol Chem. 1989, 64 (9): 5269-5282.","journal-title":"J Biol Chem"},{"key":"6475_CR10","doi-asserted-by":"publisher","first-page":"2735","DOI":"10.1002\/elps.1150181507","volume":"18","author":"RD Appel","year":"1997","unstructured":"Appel RD, Vargas JR, Palagi PM, Walther D, Hochstrasser DF: Melanie II--a third-generation software package for analysis of two-dimensional electrophoresis images: II. Algorithms. Electrophoresis. 1997, 18: 2735-2748. 10.1002\/elps.1150181507.","journal-title":"Electrophoresis"},{"key":"6475_CR11","doi-asserted-by":"publisher","first-page":"887","DOI":"10.1002\/pmic.200300421","volume":"3","author":"M Rogers","year":"2003","unstructured":"Rogers M, Graham J, Tonge RP: Statistical models of shape for the analysis of protein spots in two-dimensional electrophoresis gel images. Proteomics. 2003, 3: 887-896. 10.1002\/pmic.200300421.","journal-title":"Proteomics"},{"key":"6475_CR12","doi-asserted-by":"publisher","first-page":"336","DOI":"10.1016\/j.gpb.2012.06.005","volume":"10","author":"M Natale","year":"2012","unstructured":"Natale M, Caiazzo A, Bucci EM, Ficarra E: A novel Gaussian extrapolation approach for 2D gel electrophoresis saturated protein spots. Genomics Proteomics Bioinformatics. 2012, 10: 336-344. 10.1016\/j.gpb.2012.06.005.","journal-title":"Genomics Proteomics Bioinformatics"},{"key":"6475_CR13","doi-asserted-by":"publisher","first-page":"1864","DOI":"10.1021\/pr901014n","volume":"9","author":"E Marengo","year":"2010","unstructured":"Marengo E, Demartini M, Robotti E, Bobba M: A new algorithm for the simulation of sodium dodecil sulfate two-dimensional polyacrylamide gel electrophoresis data sets. J Proteome Res. 2010, 9: 1864-1872. 10.1021\/pr901014n.","journal-title":"J Proteome Res"},{"key":"6475_CR14","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1006\/abio.1996.0195","volume":"237","author":"HW Klafki","year":"1996","unstructured":"Klafki HW, Wiltfang J, Staufenbiel M: Electrophoretic separation of betaA4 peptides (1-40) and (1-42). Anal Biochem. 1996, 237: 24-29. 10.1006\/abio.1996.0195.","journal-title":"Anal Biochem"},{"key":"6475_CR15","doi-asserted-by":"publisher","first-page":"3815","DOI":"10.1002\/pmic.200700311","volume":"7","author":"JM Maler","year":"2007","unstructured":"Maler JM, Klafki H, Paul S, Spitzer P, Groemer TW, Henkel AW, Esselmann H, Lewczuk P, Kornhuber J, Wiltfang J: Urea-based two-dimensional electrophoresis of beta-amyloid peptides in human plasma: evidence for novel Abeta species. Proteomics. 2007, 7: 3815-3820. 10.1002\/pmic.200700311.","journal-title":"Proteomics"},{"key":"6475_CR16","doi-asserted-by":"publisher","first-page":"524","DOI":"10.1016\/j.biopsych.2003.10.014","volume":"55","author":"P Lewczuk","year":"2004","unstructured":"Lewczuk P, Esselmann H, Groemer TW, Bibl M, Maler JM, Steinacker P, Otto M, Kornhuber J, Wiltfang J: Amyloid beta peptides in cerebrospinal fluid as profiled with surface enhanced laser desorption\/ionization time-of-flight mass spectrometry: evidence of novel biomarkers in Alzheimer's disease. Biol Psychiatry. 2004, 55: 524-530. 10.1016\/j.biopsych.2003.10.014.","journal-title":"Biol Psychiatry"},{"key":"6475_CR17","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1016\/S0197-4580(03)00086-1","volume":"25","author":"P Lewczuk","year":"2004","unstructured":"Lewczuk P, Esselmann H, Otto M, Maler JM, Henkel AW, Henkel MK, Eikenberg O, Antz C, Krause W, Reulbach U, Kornhuber J, Wiltfang J: Neurochemical diagnosis of alzheimer's dementia by CSF Abeta42, Abeta42\/Abeta40 ratio and total tau. Neurobiol Aging. 2004, 25: 273-281. 10.1016\/S0197-4580(03)00086-1.","journal-title":"Neurobiol Aging"},{"key":"6475_CR18","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1016\/j.jsb.2005.06.002","volume":"151","author":"IF Sbalzarini","year":"2005","unstructured":"Sbalzarini IF, Koumoutsakos P: Feature point tracking and trajectory analysis for video imaging in cell biology. J Struct Biol. 2005, 151: 182-195. 10.1016\/j.jsb.2005.06.002.","journal-title":"J Struct Biol"},{"key":"6475_CR19","doi-asserted-by":"publisher","first-page":"298","DOI":"10.1006\/jcis.1996.0217","volume":"179","author":"JC Crocker","year":"1996","unstructured":"Crocker JC, Grier DG: Methods of digital video microscopy for colloidal studies. J Colloid Interface Sci. 1996, 179: 298-310. 10.1006\/jcis.1996.0217.","journal-title":"J Colloid Interface Sci"},{"key":"6475_CR20","doi-asserted-by":"publisher","first-page":"1567","DOI":"10.1002\/pmic.200300459","volume":"3","author":"AW Dowsey","year":"2003","unstructured":"Dowsey AW, Dunn MJ, Yang G: The role of bioinformatics in two-dimensional gel electrophoresis. Proteomics. 2003, 3: 1567-1596. 10.1002\/pmic.200300459.","journal-title":"Proteomics"},{"key":"6475_CR21","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/0165-0270(89)90104-0","volume":"29","author":"RH Lustig","year":"1989","unstructured":"Lustig RH, Pfaff DW, Mobbs CV: Considerations in the quantitative analysis of autoradiograms from 2-dimensional gels. J Neurosci Methods. 1989, 29: 17-26. 10.1016\/0165-0270(89)90104-0.","journal-title":"J Neurosci Methods"},{"key":"6475_CR22","doi-asserted-by":"publisher","first-page":"738","DOI":"10.1002\/(SICI)1522-2683(19990101)20:4\/5<738::AID-ELPS738>3.0.CO;2-2","volume":"20","author":"JX Yan","year":"1999","unstructured":"Yan JX, Sanchez JC, Tonella L, Williams KL, Hochstrasser DF: Studies of quantitative analysis of protein expression in Saccharomyces cerevisiae. Electrophoresis. 1999, 20: 738-742. 10.1002\/(SICI)1522-2683(19990101)20:4\/5<738::AID-ELPS738>3.0.CO;2-2.","journal-title":"Electrophoresis"},{"key":"6475_CR23","doi-asserted-by":"publisher","first-page":"595","DOI":"10.1021\/pr9005603","volume":"9","author":"JS Morris","year":"2010","unstructured":"Morris JS, Clark BN, Wei W, Gutstein HB: Evaluating the performance of new approaches to spot quantification and differential expression in 2-dimensional gel electrophoresis studies. J Proteome Res. 2010, 9: 595-604. 10.1021\/pr9005603.","journal-title":"J Proteome Res"},{"key":"6475_CR24","doi-asserted-by":"publisher","first-page":"601","DOI":"10.1002\/elps.1150140195","volume":"14","author":"J Vohradsky","year":"1993","unstructured":"Vohradsky J, Panek J: Quantitative analysis of gel electrophoretograms by image analysis and least squares modeling. Electrophoresis. 1993, 14: 601-612. 10.1002\/elps.1150140195.","journal-title":"Electrophoresis"},{"key":"6475_CR25","doi-asserted-by":"publisher","first-page":"2194","DOI":"10.1002\/1522-2683(200207)23:14<2194::AID-ELPS2194>3.0.CO;2-#","volume":"23","author":"B Raman","year":"2002","unstructured":"Raman B, Cheung A, Marten MR: Quantitative comparison and evaluation of two commercially available, two-dimensional electrophoresis image analysis software packages, Z3 and Melanie. Electrophoresis. 2002, 23: 2194-2202. 10.1002\/1522-2683(200207)23:14<2194::AID-ELPS2194>3.0.CO;2-#.","journal-title":"Electrophoresis"},{"key":"6475_CR26","doi-asserted-by":"publisher","first-page":"2038","DOI":"10.1002\/pmic.201000601","volume":"11","author":"P Tsakanikas","year":"2011","unstructured":"Tsakanikas P, Manolakos ES: Protein spot detection and quantification in 2-DE gel images using machine-learning methods. Proteomics. 2011, 11: 2038-2050. 10.1002\/pmic.201000601.","journal-title":"Proteomics"}],"container-title":["BMC Bioinformatics"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2105-15-181.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/1471-2105-15-181\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2105-15-181.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,7]],"date-time":"2022-04-07T16:37:54Z","timestamp":1649349474000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcbioinformatics.biomedcentral.com\/articles\/10.1186\/1471-2105-15-181"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,6,11]]},"references-count":26,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,12]]}},"alternative-id":["6475"],"URL":"https:\/\/doi.org\/10.1186\/1471-2105-15-181","relation":{},"ISSN":["1471-2105"],"issn-type":[{"value":"1471-2105","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6,11]]},"assertion":[{"value":"13 April 2013","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 May 2014","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 June 2014","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"181"}}