{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T09:12:38Z","timestamp":1761988358719},"reference-count":23,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Cell Biol"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Low concentrations of carbon monoxide (CO) protect hepatocytes against apoptosis and confers cytoprotection in several models of liver. Mitochondria are key organelles in cell death control <jats:italic>via<\/jats:italic> their membrane permeabilization and the release of pro-apoptotic factors.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Herein, we show that CO prevents mitochondrial membrane permeabilization (MMP) in liver isolated mitochondria. Direct and indirect approaches were used to evaluate MMP inhibition by CO: mitochondrial swelling, mitochondrial depolarization and inner membrane permeabilization. Additionally, CO increases mitochondrial reactive oxygen species (ROS) generation, and their scavenging, by \u00df-carotene addition, decreases CO protection, which reveals the key role of ROS. Interestingly, cytochrome c oxidase transiently responds to low concentrations of CO by decreasing its activity in the first 5 min, later on there is an increase of cytochrome c oxidase activity, which were detected up to 30 min.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>CO directly prevents mitochondrial membrane permeabilization, which might be implicated in the hepatic apoptosis inhibition by this gaseoustransmitter.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2121-12-10","type":"journal-article","created":{"date-parts":[[2011,3,9]],"date-time":"2011-03-09T15:20:51Z","timestamp":1299684051000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Carbon monoxide prevents hepatic mitochondrial membrane permeabilization"],"prefix":"10.1186","volume":"12","author":[{"given":"Cl\u00e1udia SF","family":"Queiroga","sequence":"first","affiliation":[]},{"given":"Ana S","family":"Almeida","sequence":"additional","affiliation":[]},{"given":"Paula M","family":"Alves","sequence":"additional","affiliation":[]},{"given":"Catherine","family":"Brenner","sequence":"additional","affiliation":[]},{"given":"Helena LA","family":"Vieira","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2011,3,9]]},"reference":[{"issue":"2","key":"555_CR1","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1152\/physrev.00011.2005","volume":"86","author":"SW Ryter","year":"2006","unstructured":"Ryter SW, Alam J, Choi AM: Heme oxygenase-1\/carbon monoxide: from basic science to therapeutic applications. 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