{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T16:45:48Z","timestamp":1762015548137},"reference-count":86,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2010,11,25]],"date-time":"2010-11-25T00:00:00Z","timestamp":1290643200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/2.0"},{"start":{"date-parts":[[2010,11,25]],"date-time":"2010-11-25T00:00:00Z","timestamp":1290643200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/2.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Evol Biol"],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Behaviour has been traditionally viewed as a driver of subsequent evolution because behavioural adjustments expose organisms to novel environments, which may result in a correlated evolution on other traits. In<jats:italic>Drosophila subobscura<\/jats:italic>, thermal preference and heat tolerance are linked to chromosomal inversion polymorphisms that show parallel latitudinal clines worldwide, such that \"cold-climate\" (\"warm-climate\") chromosome arrangements collectively favour a coherent response to colder (warmer) settings as flies carrying them prefer colder (warmer) conditions and have lower (higher) knock out temperatures. Yet, it is not clear whether a genetic correlation between thermal preference and heat tolerance can partially underlie such response.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We have analyzed the genetic basis of thermal preference and heat tolerance using isochromosomal lines in<jats:italic>D. subobscura<\/jats:italic>. Chromosome arrangements on the O chromosome were known to have a biometrical effect on thermal preference in a laboratory temperature gradient, and also harbour several genes involved in the heat shock response; in particular, the genes<jats:italic>Hsp68<\/jats:italic>and<jats:italic>Hsp70<\/jats:italic>. Our results corroborate that arrangements on chromosome O affect adult thermal preference in a laboratory temperature gradient, with cold-climate O<jats:sub>st<\/jats:sub>carriers displaying a lower thermal preference than their warm-climate O<jats:sub>3+4<\/jats:sub>and O<jats:sub>3+4+8<\/jats:sub>counterparts. However, these chromosome arrangements did not have any effect on adult heat tolerance and, hence, we putatively discard a genetic covariance between both traits arising from linkage disequilibrium between genes affecting thermal preference and candidate genes for heat shock resistance. Nonetheless, a possible association of juvenile thermal preference and heat resistance warrants further analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Thermal preference and heat tolerance in the isochromosomal lines of<jats:italic>D. subobscura<\/jats:italic>appear to be genetically independent, which might potentially prevent a coherent response of behaviour and physiology (i.e., coadaptation) to thermal selection. If this pattern is general to all chromosomes, then any correlation between thermal preference and heat resistance across latitudinal gradients would likely reflect a pattern of correlated selection rather than genetic correlation.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1471-2148-10-363","type":"journal-article","created":{"date-parts":[[2010,11,25]],"date-time":"2010-11-25T19:15:03Z","timestamp":1290712503000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Genetic constraints for thermal coadaptation in Drosophila subobscura"],"prefix":"10.1186","volume":"10","author":[{"given":"Olga","family":"Dolgova","sequence":"first","affiliation":[]},{"given":"Carla","family":"Rego","sequence":"additional","affiliation":[]},{"given":"Gemma","family":"Calabria","sequence":"additional","affiliation":[]},{"given":"Joan","family":"Balany\u00e0","sequence":"additional","affiliation":[]},{"given":"Marta","family":"Pascual","sequence":"additional","affiliation":[]},{"given":"Enrico L","family":"Rezende","sequence":"additional","affiliation":[]},{"given":"Mauro","family":"Santos","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2010,11,25]]},"reference":[{"key":"1578_CR1","doi-asserted-by":"publisher","DOI":"10.1093\/acprof:oso\/9780198570875.001.1","volume-title":"Thermal Adaptation. A Theoretical and Empirical Synthesis","author":"MJ Angilletta","year":"2009","unstructured":"Angilletta MJ: Thermal Adaptation. A Theoretical and Empirical Synthesis. 2009, Oxford: Oxford Univ Press"},{"key":"1578_CR2","doi-asserted-by":"publisher","first-page":"362","DOI":"10.1086\/284423","volume":"126","author":"RD Stevenson","year":"1985","unstructured":"Stevenson RD: The relative importance of behavioral and physiological adjustments controlling body temperature in terrestrial ectotherms. Am Nat. 1985, 126: 362-386. 10.1086\/284423.","journal-title":"Am Nat"},{"key":"1578_CR3","doi-asserted-by":"publisher","first-page":"1131","DOI":"10.1126\/science.214.4525.1131","volume":"214","author":"TM Casey","year":"1981","unstructured":"Casey TM, Hegel JR: Caterpillar setae: insulation for an ectotherm. Science. 1981, 214: 1131-1133. 10.1126\/science.214.4525.1131.","journal-title":"Science"},{"key":"1578_CR4","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1086\/284038","volume":"121","author":"JG Kingsolver","year":"1983","unstructured":"Kingsolver JG, Watt WB: Thermoregulatory strategies in Colias butterflies: thermal stress and limits to adaptation in temporally varying environments. Am Nat. 1983, 121: 32-35. 10.1086\/284038.","journal-title":"Am Nat"},{"key":"1578_CR5","doi-asserted-by":"publisher","first-page":"250","DOI":"10.1007\/BF00348047","volume":"50","author":"PG Willmer","year":"1981","unstructured":"Willmer PG, Unwin S: Field analyses of insect heat budget: reflectance, size and heating rates. Oecologia. 1981, 50: 250-255. 10.1007\/BF00348047.","journal-title":"Oecologia"},{"key":"1578_CR6","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1086\/346135","volume":"161","author":"RB Huey","year":"2003","unstructured":"Huey RB, Hertz PE, Sinervo B: Behavioral drive versus behavioral inertia in evolution: a null model approach. Am Nat. 2003, 161: 357-366. 10.1086\/346135.","journal-title":"Am Nat"},{"key":"1578_CR7","volume-title":"Genetics of the Evolutionary Process","author":"Th Dobzhansky","year":"1970","unstructured":"Dobzhansky Th: Genetics of the Evolutionary Process. 1970, New York: Columbia Univ Press"},{"key":"1578_CR8","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1093\/genetics\/142.1.179","volume":"142","author":"F Rodr\u00edguez-Trelles","year":"1996","unstructured":"Rodr\u00edguez-Trelles F, Alvarez G, Zapata C: Time-series analysis of seasonal changes of the O inversion polymorphism of Drosophila subobscura. Genetics. 1996, 142: 179-187.","journal-title":"Genetics"},{"key":"1578_CR9","doi-asserted-by":"publisher","first-page":"474","DOI":"10.1086\/284150","volume":"122","author":"JA Coyne","year":"1983","unstructured":"Coyne JA, Bundgaard J, Prout T: Geographic variation of tolerance to environmental stress in Drosophila pseudoobscura. Am Nat. 1983, 122: 474-488. 10.1086\/284150.","journal-title":"Am Nat"},{"key":"1578_CR10","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1016\/S0306-4565(02)00057-8","volume":"28","author":"AA Hoffmann","year":"2003","unstructured":"Hoffmann AA, S\u00f8rensen JG, Loeschcke V: Adaptation of Drosophila to temperature extremes: bringing together quantitative and molecular approaches. J Therm Biol. 2003, 28: 175-216. 10.1016\/S0306-4565(02)00057-8.","journal-title":"J Therm Biol"},{"key":"1578_CR11","doi-asserted-by":"publisher","first-page":"870","DOI":"10.1242\/jeb.037630","volume":"213","author":"AA Hoffmann","year":"2010","unstructured":"Hoffmann AA: Physiological climatic limits in Drosophila: patterns and implications. J Exp Biol. 2010, 213: 870-880. 10.1242\/jeb.037630.","journal-title":"J Exp Biol"},{"key":"1578_CR12","doi-asserted-by":"publisher","first-page":"1715","DOI":"10.1890\/09-0097.1","volume":"90","author":"RB Huey","year":"2009","unstructured":"Huey RB, Pascual M: Partial thermoregulatory compensation along a latitudinal cline in a rapidly evolving invasive species. Drosophila subobscura in North America. Ecology. 2009, 90: 1715-1720. 10.1890\/09-0097.1.","journal-title":"Ecology"},{"key":"1578_CR13","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1016\/S0169-5347(03)00014-4","volume":"18","author":"BJ Sinclair","year":"2003","unstructured":"Sinclair BJ, Vernon P, Klok CJ, Chown SL: Insects at low temperatures: an ecological perspective. Trends Ecol Evol. 2003, 18: 257-262. 10.1016\/S0169-5347(03)00014-4.","journal-title":"Trends Ecol Evol"},{"key":"1578_CR14","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1146\/annurev-physiol-021909-135900","volume":"72","author":"GE Hofmann","year":"2010","unstructured":"Hofmann GE, Todgham AE: Living in the now: physiological mechanisms to tolerate a rapidly changing environment. Annu Rev Physiol. 2010, 72: 127-145. 10.1146\/annurev-physiol-021909-135900.","journal-title":"Annu Rev Physiol"},{"key":"1578_CR15","doi-asserted-by":"publisher","first-page":"1098","DOI":"10.2307\/2409194","volume":"41","author":"RB Huey","year":"1987","unstructured":"Huey RB, Bennett AF: Phylogenetic studies of coadaptation: preferred temperatures versus optimal performance temperatures of lizards. Evolution. 1987, 41: 1098-1115. 10.2307\/2409194.","journal-title":"Evolution"},{"key":"1578_CR16","doi-asserted-by":"publisher","first-page":"282","DOI":"10.1086\/499990","volume":"79","author":"MJ Angilletta","year":"2006","unstructured":"Angilletta MJ, Bennett AF, Guderley H, Navas CA, Seebacher F, Wilson RS: Coadaptation: a unified principle in evolutionary thermal biology. Physiol Biochem Zool. 2006, 79: 282-294. 10.1086\/499990.","journal-title":"Physiol Biochem Zool"},{"key":"1578_CR17","doi-asserted-by":"publisher","DOI":"10.4159\/harvard.9780674865327","volume-title":"Animal Species and Evolution","author":"E Mayr","year":"1963","unstructured":"Mayr E: Animal Species and Evolution. 1963, Cambridge, Harvard Univ Press"},{"key":"1578_CR18","doi-asserted-by":"publisher","first-page":"246","DOI":"10.1139\/g89-042","volume":"31","author":"FJ Ayala","year":"1989","unstructured":"Ayala FJ, Serra L, Prevosti A: A grand experiment in evolution Drosophila subobscura colonization of the Americas. Genome. 1989, 31: 246-255.","journal-title":"Genome"},{"key":"1578_CR19","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1007\/s10709-006-9010-z","volume":"129","author":"AA Hoffmann","year":"2007","unstructured":"Hoffmann AA, Weeks AR: Climatic selection on genes and traits after 100 year-old invasion: a critical look at the temperate-tropical clines in Drosophila melanogaster from eastern Australia. Genetica. 2007, 129: 133-147. 10.1007\/s10709-006-9010-z.","journal-title":"Genetica"},{"key":"1578_CR20","doi-asserted-by":"publisher","first-page":"308","DOI":"10.1126\/science.287.5451.308","volume":"287","author":"RB Huey","year":"2000","unstructured":"Huey RB, Gilchrist GW, Carlson ML, Berrigan D, Serra L: Rapid evolution of a geographical cline in size in an introduced fly. Science. 2000, 287: 308-309. 10.1126\/science.287.5451.308.","journal-title":"Science"},{"key":"1578_CR21","doi-asserted-by":"publisher","first-page":"1837","DOI":"10.1111\/j.0014-3820.2003.tb00591.x","volume":"57","author":"J Balany\u00e0","year":"2003","unstructured":"Balany\u00e0 J, Serra L, Gilchrist GW, Huey RB, Pascual M, Mestres F, Sol\u00e9 E: Evolutionary pace of chromosomal polymorphism in colonizing populations of Drosophila subobscura: an evolutionary time series. Evolution. 2003, 57: 1837-1845.","journal-title":"Evolution"},{"key":"1578_CR22","doi-asserted-by":"publisher","first-page":"103","DOI":"10.3354\/cr00869","volume":"43","author":"EL Rezende","year":"2010","unstructured":"Rezende EL, Balany\u00e0 J, Rodr\u00edguez-Trelles F, Rego C, Fragata I, Matos M, Serra L, Santos M: Climate change and chromosomal inversions in Drosophila subobscura. Clim Res. 2010, 43: 103-114. 10.3354\/cr00869.","journal-title":"Clim Res"},{"key":"1578_CR23","doi-asserted-by":"publisher","first-page":"513","DOI":"10.1111\/j.1752-4571.2008.00040.x","volume":"1","author":"G Gilchrist","year":"2008","unstructured":"Gilchrist G, Jeffers L, West B, Folk D, Suess J, Huey RB: Clinal patterns of desiccation and starvation resistance in ancestral and invading populations of Drosophila subobscura. Evol Appl. 2008, 1: 513-23. 10.1111\/j.1752-4571.2008.00040.x.","journal-title":"Evol Appl"},{"key":"1578_CR24","doi-asserted-by":"publisher","first-page":"566","DOI":"10.1111\/j.0014-3820.2003.tb01548.x","volume":"57","author":"FCF Calboli","year":"2003","unstructured":"Calboli FCF, Gilchrist GW, Partridge L: Different cell size and cell number contribution in two newly established and one ancient body size cline of Drosophila subobscura. Evolution. 2003, 57: 566-573.","journal-title":"Evolution"},{"key":"1578_CR25","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1023\/A:1013358931816","volume":"112-113","author":"GW Gilchrist","year":"2001","unstructured":"Gilchrist GW, Huey RB, Serra L: Rapid evolution of wing size clines in Drosophila subobscura. Genetica. 2001, 112-113: 273-286. 10.1023\/A:1013358931816.","journal-title":"Genetica"},{"key":"1578_CR26","doi-asserted-by":"publisher","first-page":"144","DOI":"10.2307\/2410929","volume":"52","author":"E Betr\u00e1n","year":"1998","unstructured":"Betr\u00e1n E, Santos M, Ruiz A: Antagonistic pleiotropic effect of second-chromosome inversions on body size and early life-history traits in Drosophila buzzatii. Evolution. 1998, 52: 144-154. 10.2307\/2410929.","journal-title":"Evolution"},{"key":"1578_CR27","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1126\/science.1063656","volume":"294","author":"JR Etterson","year":"2001","unstructured":"Etterson JR, Shaw RG: Constraint to adaptive evolution in response to global warming. Science. 2001, 294: 151-154. 10.1126\/science.1063656.","journal-title":"Science"},{"key":"1578_CR28","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1093\/genetics\/105.4.935","volume":"105","author":"A Fontdevila","year":"1983","unstructured":"Fontdevila A, Zapata C, Alvarez G, Sanchez L, M\u00e9ndez J, Enriquez I: Genetic coadaptation in the chromosomal polymorphism of Drosophila subobscura. I. Seasonal changes of gametic disequilibrium in a natural population. Genetics. 1983, 105: 935-955.","journal-title":"Genetics"},{"key":"1578_CR29","doi-asserted-by":"publisher","first-page":"839","DOI":"10.1111\/j.0014-3820.2003.tb00295.x","volume":"57","author":"F Rodr\u00edguez-Trelles","year":"2003","unstructured":"Rodr\u00edguez-Trelles F: Seasonal cycles of allozyme-by-chromosomal-inversion gametic disequilibrium in Drosophila subobscura. Evolution. 2003, 57: 839-848.","journal-title":"Evolution"},{"key":"1578_CR30","doi-asserted-by":"publisher","first-page":"112","DOI":"10.1111\/j.1420-9101.2009.01873.x","volume":"23","author":"I Fragata","year":"2010","unstructured":"Fragata I, Balany\u00e0 J, Rego C, Matos M, Rezende EL, Santos M: Contrasting patterns of phenotypic variation linked to chromosomal inversions in native and colonizing populations of Drosophila subobscura. J Evol Biol. 2010, 23: 112-123. 10.1111\/j.1420-9101.2009.01873.x.","journal-title":"J Evol Biol"},{"key":"1578_CR31","doi-asserted-by":"publisher","first-page":"E66","DOI":"10.1086\/499383","volume":"167","author":"P Nosil","year":"2006","unstructured":"Nosil P, Crespi BJ, Sandoval CP, Kirkpatrick M: Migration and the genetic covariance between habitat preference and performance. Am Nat. 2006, 167: E66-E78. 10.1086\/499383.","journal-title":"Am Nat"},{"key":"1578_CR32","doi-asserted-by":"publisher","first-page":"513","DOI":"10.1007\/s10682-008-9252-6","volume":"23","author":"RE Duckworth","year":"2009","unstructured":"Duckworth RE: The role of behavior in evolution: a search for mechanism. Evol Ecol. 2009, 23: 513-531. 10.1007\/s10682-008-9252-6.","journal-title":"Evol Ecol"},{"key":"1578_CR33","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1016\/j.jtherbio.2008.11.007","volume":"34","author":"ME Dillon","year":"2009","unstructured":"Dillon ME, Wang G, Garrity PA, Huey RB: Thermal preference in Drosophila. J Therm Biol. 2009, 34: 109-119. 10.1016\/j.jtherbio.2008.11.007.","journal-title":"J Therm Biol"},{"key":"1578_CR34","doi-asserted-by":"publisher","first-page":"385","DOI":"10.1111\/j.1558-5646.2009.00835.x","volume":"64","author":"C Rego","year":"2010","unstructured":"Rego C, Balany\u00e0 J, Fragata I, Matos M, Rezende EL, Santos M: Clinal patterns of chromosomal inversion polymorphisms in Drosophila subobscura are partly associated with thermal preferences and heat stress resistance. Evolution. 2010, 64: 385-397. 10.1111\/j.1558-5646.2009.00835.x.","journal-title":"Evolution"},{"key":"1578_CR35","doi-asserted-by":"publisher","first-page":"625","DOI":"10.1046\/j.1420-9101.1992.5040625.x","volume":"5","author":"P Menozzi","year":"1992","unstructured":"Menozzi P, Krimbas CB: The inversion polymorphism of Drosophila subobscura revisited: synthetic maps of gene arrangement frequencies and their interpretation. J Evol Biol. 1992, 5: 625-641. 10.1046\/j.1420-9101.1992.5040625.x.","journal-title":"J Evol Biol"},{"key":"1578_CR36","first-page":"127","volume-title":"Drosophila Inversion Polymorphism","author":"CB Krimbas","year":"1992","unstructured":"Krimbas CB: The inversion polymorphism of Drosophila subobscura. Drosophila Inversion Polymorphism. Edited by: Krimbas CB, Powell JR. 1992, Boca Raton: CRC Press, 127-220."},{"key":"1578_CR37","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1093\/genetics\/86.4.835","volume":"86","author":"D Sperlich","year":"1977","unstructured":"Sperlich D, Feuerbach-Mravlag H, Lange P, Michaelidis A, Pentzos-Daponte A: Genetic load and viability distribution in central and marginal populations of Drosophila subobscura. Genetics. 1977, 86: 835-848.","journal-title":"Genetics"},{"key":"1578_CR38","doi-asserted-by":"publisher","first-page":"795","DOI":"10.1007\/s10709-010-9461-0","volume":"138","author":"C Pegueroles","year":"2010","unstructured":"Pegueroles C, Ara\u00faz PA, Pascual M, Mestres F: A recombination survey using microsatellites: the O chromosome of Drosophila subobscura. Genetica. 2010, 138: 795-804. 10.1007\/s10709-010-9461-0.","journal-title":"Genetica"},{"key":"1578_CR39","doi-asserted-by":"crossref","DOI":"10.1093\/oso\/9780195076912.001.0001","volume-title":"Progress and Prospects in Evolutionary Biology. The Drosophila Model","author":"JR Powell","year":"1997","unstructured":"Powell JR: Progress and Prospects in Evolutionary Biology. The Drosophila Model. 1997, New York: Oxford Univ Press"},{"key":"1578_CR40","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1007\/s10577-010-9112-4","volume":"18","author":"J Santos","year":"2010","unstructured":"Santos J, Serra L, Sol\u00e9 E, Pascual M: FISH mapping of microsatellite loci from Drosophila subobscura and its comparison to related species. Chromosome Res. 2010, 18: 213-226. 10.1007\/s10577-010-9112-4.","journal-title":"Chromosome Res"},{"key":"1578_CR41","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1186\/1471-2148-7-42","volume":"7","author":"H Laayouni","year":"2007","unstructured":"Laayouni H, Garc\u00eda-Franco F, Ch\u00e1vez-Sandoval BE, Trotta V, Beltran S, Corominas M, Santos M: Thermal evolution of gene expression profiles in Drosophila subobscura. BMC Evol Biol. 2007, 7: 42-10.1186\/1471-2148-7-42.","journal-title":"BMC Evol Biol"},{"key":"1578_CR42","doi-asserted-by":"publisher","first-page":"870","DOI":"10.1139\/g92-132","volume":"35","author":"MD Molt\u00f3","year":"1992","unstructured":"Molt\u00f3 MD, Pascual L, Mart\u00ednez-Sebasti\u00e1n MJ, de Frutos R: Genetic analysis of the heat shock response in three Drosophila species of the obscura group. Genome. 1992, 35: 870-880.","journal-title":"Genome"},{"key":"1578_CR43","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1007\/s004120050287","volume":"107","author":"JB Cuenca","year":"1998","unstructured":"Cuenca JB, Galindo MI, Saura AO, Sorsa V, de Frutos R: Ultrastructure of regions containing homologous loci in polytene chromosomes of Drosophila melanogaster and Drosophila subobscura. Chromosoma. 1998, 107: 113-126. 10.1007\/s004120050287.","journal-title":"Chromosoma"},{"key":"1578_CR44","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1007\/BF02568093","volume":"9","author":"E Kunze-M\u00fchl","year":"1958","unstructured":"Kunze-M\u00fchl E, M\u00fcller E: Weitere Untersuchungen \u00fcber die chromosomale Struktur und nat\u00fcrlichen Strukturtypen von D. subobscura. Chromosoma. 1958, 9: 559-570. 10.1007\/BF02568093.","journal-title":"Chromosoma"},{"key":"1578_CR45","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1098\/rstb.1993.0026","volume":"339","author":"DA Parsell","year":"1993","unstructured":"Parsell DA, Taulien J, Lindquist S: The role of heat-shock proteins in thermotolerance. Phil Trans Roy Soc London Series B. 1993, 339: 279-286. 10.1098\/rstb.1993.0026.","journal-title":"Phil Trans Roy Soc London Series B"},{"key":"1578_CR46","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1379\/1466-1268(1999)004<0243:ACOHEA>2.3.CO;2","volume":"4","author":"RA Krebs","year":"1999","unstructured":"Krebs RA: A comparison of Hsp70 expression and thermotolerance in adults and larvae of three Drosophila species. Cell Stress Chaperones. 1999, 4: 243-249. 10.1379\/1466-1268(1999)004<0243:ACOHEA>2.3.CO;2.","journal-title":"Cell Stress Chaperones"},{"key":"1578_CR47","doi-asserted-by":"publisher","first-page":"1796","DOI":"10.1111\/j.0014-3820.2002.tb00193.x","volume":"56","author":"BR Bettencourt","year":"2002","unstructured":"Bettencourt BR, Kim I, Hoffmann AA, Feder ME: Response to natural and laboratory selection at the Drosophila hsp70 genes. Evolution. 2002, 56: 1796-1801.","journal-title":"Evolution"},{"key":"1578_CR48","doi-asserted-by":"crossref","first-page":"1615","DOI":"10.1093\/genetics\/143.4.1615","volume":"143","author":"G McColl","year":"1996","unstructured":"McColl G, Hoffmann AA, McKechnie SW: Response of two heat shock genes to selection for knockdown heat resistance in Drosophila melanogaster. Genetics. 1996, 143: 1615-1627.","journal-title":"Genetics"},{"key":"1578_CR49","doi-asserted-by":"publisher","first-page":"841","DOI":"10.1111\/j.1420-9101.2004.00721.x","volume":"17","author":"M Santos","year":"2004","unstructured":"Santos M, Iriarte PF, C\u00e9spedes W, Balany\u00e0 J, Fontdevila A, Serra L: Swift laboratory thermal evolution of wing shape (but not size) in Drosophila subobscura and its relationship with chromosomal inversion polymorphism. J Evol Biol. 2004, 17: 841-855. 10.1111\/j.1420-9101.2004.00721.x.","journal-title":"J Evol Biol"},{"key":"1578_CR50","doi-asserted-by":"publisher","first-page":"258","DOI":"10.1086\/427093","volume":"165","author":"M Santos","year":"2005","unstructured":"Santos M, C\u00e9spedes W, Balany\u00e0 J, Trotta V, Calboli FCF, Fontdevila A, Serra L: Temperature-related genetic changes in laboratory populations of Drosophila subobscura: evidence against simple climatic-based explanations for latitudinal clines. Am Nat. 2005, 165: 258-273. 10.1086\/427093.","journal-title":"Am Nat"},{"key":"1578_CR51","doi-asserted-by":"publisher","first-page":"2361","DOI":"10.1111\/j.1420-9101.2007.01408.x","volume":"20","author":"M Santos","year":"2007","unstructured":"Santos M: Evolution of total net fitness in thermal lines: Drosophila subobscura likes it \"warm\". J Evol Biol. 2007, 20: 2361-2370. 10.1111\/j.1420-9101.2007.01408.x.","journal-title":"J Evol Biol"},{"key":"1578_CR52","doi-asserted-by":"publisher","first-page":"830","DOI":"10.1111\/j.0014-3820.2002.tb01393.x","volume":"56","author":"E Sol\u00e9","year":"2002","unstructured":"Sol\u00e9 E, Balany\u00e0 J, Sperlich D, Serra L: Long-term changes in the chromosomal inversion polymorphism of Drosophila subobscura. I. Mediterranean populations from southwestern Europe. Evolution. 2002, 56: 830-835.","journal-title":"Evolution"},{"key":"1578_CR53","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1007\/BF01508935","volume":"45","author":"A Prevosti","year":"1974","unstructured":"Prevosti A: Chromosomal inversion polymorphism in the southwestern range of Drosophila subobscura distribution area. Genetica. 1974, 45: 111-124. 10.1007\/BF01508935.","journal-title":"Genetica"},{"key":"1578_CR54","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1111\/j.1439-0469.2004.00274.x","volume":"42","author":"J Balany\u00e0","year":"2004","unstructured":"Balany\u00e0 J, Sol\u00e9 E, Oller JM, Sperlich D, Serra L: Long-term changes in the chromosomal inversion polymorphism of Drosophila subobscura. II. European populations. J Zool Syst Evol Res. 2004, 42: 191-201. 10.1111\/j.1439-0469.2004.00274.x.","journal-title":"J Zool Syst Evol Res"},{"key":"1578_CR55","doi-asserted-by":"crossref","first-page":"1573","DOI":"10.1534\/genetics.104.032748","volume":"69","author":"A Munt\u00e9","year":"2005","unstructured":"Munt\u00e9 A, Rozas J, Aguad\u00e9 M, Segarra C: Chromosomal inversion polymorphism leads to extensive genetic structure: a multilocus survey in Drosophila subobscura. Genetics. 2005, 69: 1573-1581.","journal-title":"Genetics"},{"key":"1578_CR56","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1017\/S0016672301004955","volume":"77","author":"P Andolfatto","year":"2001","unstructured":"Andolfatto P, Depaulis F, Navarro A: Inversion polymorphism and nucleotide variability in Drosophila. Genet Res. 2001, 77: 1-8. 10.1017\/S0016672301004955.","journal-title":"Genet Res"},{"key":"1578_CR57","doi-asserted-by":"publisher","first-page":"931","DOI":"10.1093\/molbev\/msg099","volume":"20","author":"H Laayouni","year":"2003","unstructured":"Laayouni H, Hasson E, Santos M, Fontdevila A: Inversion polymorphism and nucleotide variability in different regions of the second-chromosome in Drosophila buzzatii. Mol Biol Evol. 2003, 20: 931-944. 10.1093\/molbev\/msg099.","journal-title":"Mol Biol Evol"},{"key":"1578_CR58","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1186\/1471-2148-5-7","volume":"5","author":"M Santos","year":"2005","unstructured":"Santos M, Iriarte PF, Cespedes W: Genetics and geometry of canalization and developmental stability in Drosophila subobscura. BMC Evol Biol. 2005, 5: 7-10.1186\/1471-2148-5-7.","journal-title":"BMC Evol Biol"},{"key":"1578_CR59","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1266\/jjg.69.67","volume":"69","author":"AH Yamamoto","year":"1994","unstructured":"Yamamoto AH: Temperature preference of Drosophila immigrans and D. virilis: Intra- and inter-population genetic variation. Jpn J Genet. 1994, 69: 67-76. 10.1266\/jjg.69.67.","journal-title":"Jpn J Genet"},{"key":"1578_CR60","doi-asserted-by":"publisher","first-page":"796","DOI":"10.1086\/285573","volume":"142","author":"PE Hertz","year":"1993","unstructured":"Hertz PE, Huey RB, Stevenson RD: Evaluating temperature regulation by field-active ectotherms: the fallacy of the inappropriate question. Am Nat. 1993, 142: 796-818. 10.1086\/285573.","journal-title":"Am Nat"},{"key":"1578_CR61","volume-title":"Introduction to Quantitative Genetics","author":"DS Falconer","year":"1996","unstructured":"Falconer DS, Mackay TF: Introduction to Quantitative Genetics. 1996, Essex, England: Longman, 4","edition":"4"},{"key":"1578_CR62","doi-asserted-by":"publisher","first-page":"1773","DOI":"10.1126\/science.1131002","volume":"313","author":"J Balany\u00e0","year":"2006","unstructured":"Balany\u00e0 J, Oller JM, Huey RB, Gilchrist GW, Serra L: Global genetic change tracks global climate warming in Drosophila subobscura. Science. 2006, 313: 1773-1775. 10.1126\/science.1131002.","journal-title":"Science"},{"key":"1578_CR63","doi-asserted-by":"publisher","first-page":"1025","DOI":"10.1046\/j.1461-0248.2003.00528.x","volume":"6","author":"JG S\u00f8rensen","year":"2003","unstructured":"S\u00f8rensen JG, Kristensen TN, Loeschcke V: The evolutionary and ecological role of heat shock proteins. Ecol Lett. 2003, 6: 1025-1037. 10.1046\/j.1461-0248.2003.00528.x.","journal-title":"Ecol Lett"},{"key":"1578_CR64","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1002\/jez.573","volume":"313A","author":"LT Jensen","year":"2010","unstructured":"Jensen LT, Cockerell FE, Kristensen TN, Rako L, Loeschcke V, McKechnie SW, Hoffmann AA: Adult heat tolerance variation in Drosophila melanogaster is not related with Hsp70 expression. J Exp Zool. 2010, 313A: 35-44. 10.1002\/jez.573.","journal-title":"J Exp Zool"},{"key":"1578_CR65","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1006\/anbe.1996.0333","volume":"53","author":"ME Feder","year":"1997","unstructured":"Feder ME, Blair N, Figueras H: Oviposition site selection: unresponsiveness of Drosophila to cues of potential thermal stress. Anim Behav. 1997, 53: 585-588. 10.1006\/anbe.1996.0333.","journal-title":"Anim Behav"},{"key":"1578_CR66","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1111\/j.1365-2435.2008.01481.x","volume":"23","author":"SL Chown","year":"2009","unstructured":"Chown SL, Jumbam KR, S\u00f8rensen JG, Terblanche JS: Phenotypic variance, plasticity and heritability estimates of critical thermal limits depend on methodological context. Funct Ecol. 2009, 23: 133-140. 10.1111\/j.1365-2435.2008.01481.x.","journal-title":"Funct Ecol"},{"key":"1578_CR67","volume-title":"Funct Ecol","author":"EL Rezende","year":"2010","unstructured":"Rezende EL, Tejedo M, Santos M: Estimating the adaptive potential of critical thermal limits: methodological problems and evolutionary implications. Funct Ecol. 2010"},{"key":"1578_CR68","doi-asserted-by":"publisher","first-page":"694","DOI":"10.1111\/j.1365-2435.2009.01666.x","volume":"24","author":"KA Mitchell","year":"2010","unstructured":"Mitchell KA, Hoffmann AA: Thermal ramping rate influences evolutionary potential and species differences for upper thermal limits in Drosophila. Funct Ecol. 2010, 24: 694-700. 10.1111\/j.1365-2435.2009.01666.x.","journal-title":"Funct Ecol"},{"key":"1578_CR69","doi-asserted-by":"publisher","first-page":"833","DOI":"10.1016\/S0764-4469(97)85020-2","volume":"320","author":"B Moreteau","year":"1997","unstructured":"Moreteau B, Morin J-P, Gibert P, Petavy G, Pla E, David JR: Evolutionary changes of nonlinear reaction norms according to thermal adaptation: a comparison of two Drosophila species. C R Acad Sci Paris. 1997, 320: 833-841.","journal-title":"C R Acad Sci Paris"},{"key":"1578_CR70","doi-asserted-by":"publisher","first-page":"2006","DOI":"10.1111\/j.1420-9101.2006.01139.x","volume":"19","author":"M Santos","year":"2006","unstructured":"Santos M, Brites D, Laayouni L: Thermal evolution of pre-adult life history traits, geometric size and shape, and developmental stability in Drosophila subobscura. J Evol Biol. 2006, 19: 2006-2021. 10.1111\/j.1420-9101.2006.01139.x.","journal-title":"J Evol Biol"},{"key":"1578_CR71","doi-asserted-by":"publisher","first-page":"619","DOI":"10.2307\/2411097","volume":"52","author":"OL Bubli","year":"1998","unstructured":"Bubli OL, Imasheva AG, Loeschcke V: Selection for knockdown resistance to heat in Drosophila melanogaster at high and low larval densities. Evolution. 1998, 52: 619-625. 10.2307\/2411097.","journal-title":"Evolution"},{"key":"1578_CR72","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1016\/S0306-4565(99)00096-0","volume":"25","author":"JS Kari","year":"2000","unstructured":"Kari JS, Huey RB: Size and seasonal temperature in free-ranging Drosophila subobscura. J Therm Biol. 2000, 25: 267-272. 10.1016\/S0306-4565(99)00096-0.","journal-title":"J Therm Biol"},{"key":"1578_CR73","doi-asserted-by":"publisher","first-page":"1301","DOI":"10.1016\/S0022-1910(01)00119-6","volume":"47","author":"JG S\u00f8rensen","year":"2001","unstructured":"S\u00f8rensen JG, Loeschcke V: Larval crowding in Drosophila melanogaster induces Hsp70 expression, and leads to increased adult longevity and adult thermal stress resistance. J Insect Physiol. 2001, 47: 1301-1307. 10.1016\/S0022-1910(01)00119-6.","journal-title":"J Insect Physiol"},{"key":"1578_CR74","doi-asserted-by":"publisher","first-page":"208","DOI":"10.1111\/j.1439-0469.2004.00273.x","volume":"42","author":"V Savkovi\u0107","year":"2004","unstructured":"Savkovi\u0107 V, Stamenkovic-Radak M, Andjelkovic M: Diurnal variability of gene arrangement frequencies in Drosophila subobscura populations from two habitats. J Zool Syst Evol Res. 2004, 42: 208-214. 10.1111\/j.1439-0469.2004.00273.x.","journal-title":"J Zool Syst Evol Res"},{"key":"1578_CR75","volume-title":"Genetics of Populations","author":"PW Hedick","year":"2000","unstructured":"Hedick PW: Genetics of Populations. 2000, Boston: Jones and Bartlett Publ, 2","edition":"2"},{"key":"1578_CR76","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1007\/BF00128411","volume":"63","author":"R de Frutos","year":"1984","unstructured":"de Frutos R, Prevosti A: Temporal changes of chromosomal polymorphism in natural populations of Drosophila subobscura. Genetica. 1984, 63: 181-187. 10.1007\/BF00128411.","journal-title":"Genetica"},{"key":"1578_CR77","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1007\/BF02715812","volume":"82","author":"FP Iriarte","year":"2003","unstructured":"Iriarte FP, C\u00e9spedes W, Santos M: Quantitative-genetic analysis of wing form and bilateral asymmetry in isochromosomal lines of Drosophila subobscura using the Procrustes methods. J Genet. 2003, 82: 95-113. 10.1007\/BF02715812.","journal-title":"J Genet"},{"key":"1578_CR78","doi-asserted-by":"publisher","first-page":"1823","DOI":"10.2307\/2409511","volume":"44","author":"F Mestres","year":"1990","unstructured":"Mestres F, Pegueroles G, Prevosti A, Serra L: Colonization of America by Drosophila subobscura: lethal genes and the problem of the O5 inversion. Evolution. 1990, 44: 1823-1836. 10.2307\/2409511.","journal-title":"Evolution"},{"key":"1578_CR79","doi-asserted-by":"publisher","first-page":"1553","DOI":"10.1139\/z97-782","volume":"75","author":"WI Lutterschmidt","year":"1997","unstructured":"Lutterschmidt WI, Hutchinson VH: The critical thermal maximum: data to support the onset of spasms as the definitive end point. Can J Zool. 1997, 75: 1553-1560. 10.1139\/z97-782.","journal-title":"Can J Zool"},{"key":"1578_CR80","volume-title":"Randomization Tests","author":"ES Edgington","year":"1995","unstructured":"Edgington ES: Randomization Tests. 1995, New York: Marcel Dekker, 3","edition":"3"},{"key":"1578_CR81","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-009-5787-9","volume-title":"Introduction to Biometrical Genetics","author":"K Mather","year":"1977","unstructured":"Mather K, Jinks JL: Introduction to Biometrical Genetics. 1977, London: Chapman and Hall"},{"key":"1578_CR82","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4899-3406-2","volume-title":"Biometrical Genetics: The Study of Continuous Variation","author":"K Mather","year":"1982","unstructured":"Mather K, Jinks JL: Biometrical Genetics: The Study of Continuous Variation. 1982, London: Chapman and Hall, 3","edition":"3"},{"key":"1578_CR83","volume-title":"Biometry","author":"RR Sokal","year":"1995","unstructured":"Sokal RR, Rohlf FJ: Biometry. 1995, New York: Freeman, 3","edition":"3"},{"key":"1578_CR84","volume-title":"The Language of Technical Computing","author":"MathWorks Inc","year":"2007","unstructured":"MathWorks Inc: Matlab, V. 7.5.0.342 (R2007b). The Language of Technical Computing. 2007, [http:\/\/www.mathworks.com]"},{"key":"1578_CR85","volume-title":"STATISTICA (data analysis software system), version 9","author":"StatSoft Inc","year":"2009","unstructured":"StatSoft Inc: STATISTICA (data analysis software system), version 9. 2009, [http:\/\/www.statsoft.com]"},{"key":"1578_CR86","volume-title":"SPSS for Windows","author":"SPSS Inc","year":"2006","unstructured":"SPSS Inc: SPSS for Windows. 2006, [http:\/\/www.spss.com]"}],"container-title":["BMC Evolutionary Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2148-10-363.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/1471-2148-10-363\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2148-10-363.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T13:57:43Z","timestamp":1711979863000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcevolbiol.biomedcentral.com\/articles\/10.1186\/1471-2148-10-363"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,11,25]]},"references-count":86,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2010,12]]}},"alternative-id":["1578"],"URL":"https:\/\/doi.org\/10.1186\/1471-2148-10-363","relation":{},"ISSN":["1471-2148"],"issn-type":[{"value":"1471-2148","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,11,25]]},"assertion":[{"value":"12 July 2010","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 November 2010","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 November 2010","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"363"}}