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Morphology and 18S rRNA phylogeny suggest that the microaerophilic amoeboflagellate <jats:italic>Psalteriomonas lanterna<\/jats:italic>, which possesses hydrogenosomes and elusive \"modified mitochondria\", belongs to the Heterolobosea, a taxon that consists predominantly of aerobic, mitochondriate organisms. This taxon is rather unrelated to taxa with hitherto studied hydrogenosomes.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Electron microscopy of <jats:italic>P. lanterna<\/jats:italic> flagellates reveals a large globule in the centre of the cell that is build up from stacks of some 20 individual hydrogenosomes. The individual hydrogenosomes are surrounded by a double membrane that encloses a homogeneous, dark staining matrix lacking cristae. The \"modified mitochondria\" are found in the cytoplasm of the cell and are surrounded by 1-2 cisterns of rough endoplasmatic reticulum, just as the mitochondria of certain related aerobic Heterolobosea. The ultrastructure of the \"modified mitochondria\" and hydrogenosomes is very similar, and they have the same size distribution as the hydrogenosomes that form the central stack.<\/jats:p>\n            <jats:p>The phylogenetic analysis of selected EST sequences (Hsp60, Propionyl-CoA carboxylase) supports the phylogenetic position of <jats:italic>P. lanterna<\/jats:italic> close to aerobic Heterolobosea (<jats:italic>Naegleria gruberi<\/jats:italic>). Moreover, this analysis also confirms the identity of several mitochondrial or hydrogenosomal key-genes encoding proteins such as a Hsp60, a pyruvate:ferredoxin oxidoreductase, a putative ADP\/ATP carrier, a mitochondrial complex I subunit (51 KDa), and a [FeFe] hydrogenase.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>Comparison of the ultrastructure of the \"modified mitochondria\" and hydrogenosomes strongly suggests that both organelles are just two morphs of the same organelle. The EST studies suggest that the hydrogenosomes of <jats:italic>P. lanterna<\/jats:italic> are physiologically similar to the hydrogenosomes of <jats:italic>Trichomonas vaginalis<\/jats:italic> and <jats:italic>Trimastix pyriformis<\/jats:italic>. Phylogenetic analysis of the ESTs confirms the relationship of <jats:italic>P. lanterna<\/jats:italic> with its aerobic relative, the heterolobosean amoeboflagellate <jats:italic>Naegleria gruberi<\/jats:italic>, corroborating the evolution of hydrogenosomes from a common, mitochondriate ancestor.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2148-9-287","type":"journal-article","created":{"date-parts":[[2009,12,10]],"date-time":"2009-12-10T12:49:05Z","timestamp":1260449345000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["The hydrogenosomes of Psalteriomonas lanterna"],"prefix":"10.1186","volume":"9","author":[{"given":"Rob M","family":"de Graaf","sequence":"first","affiliation":[]},{"given":"Isabel","family":"Duarte","sequence":"additional","affiliation":[]},{"given":"Theo A","family":"van Alen","sequence":"additional","affiliation":[]},{"given":"Jan WP","family":"Kuiper","sequence":"additional","affiliation":[]},{"given":"Klaas","family":"Schotanus","sequence":"additional","affiliation":[]},{"given":"J\u00f6rg","family":"Rosenberg","sequence":"additional","affiliation":[]},{"given":"Martijn A","family":"Huynen","sequence":"additional","affiliation":[]},{"given":"Johannes HP","family":"Hackstein","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2009,12,9]]},"reference":[{"issue":"8","key":"1201_CR1","doi-asserted-by":"publisher","first-page":"580","DOI":"10.1016\/j.cub.2008.03.037","volume":"18","author":"A Stechmann","year":"2008","unstructured":"Stechmann A, Hamblin K, Perez-Brocal V, Gaston D, Richmond GS, Giezen van der M, Clark CG, Roger AJ: Organelles in Blastocystis that blur the distinction between mitochondria and hydrogenosomes. 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