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To identify genes responsible for AsA content in tomato fruit, transcriptomic studies followed by clustering analysis were applied to two groups of fruits with contrasting AsA content. These fruits were identified after AsA profiling of an F8 Recombinant Inbred Line (RIL) population generated from a cross between the domesticated species<jats:italic>Solanum lycopersicum<\/jats:italic>and the wild relative<jats:italic>Solanum pimpinellifollium<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found large variability in AsA content within the RIL population with individual RILs with up to 4-fold difference in AsA content. Transcriptomic analysis identified genes whose expression correlated either positively (<jats:italic>PVC<\/jats:italic>genes) or negatively (<jats:italic>NVC<\/jats:italic>genes) with the AsA content of the fruits. Cluster analysis using SOTA allowed the identification of subsets of co-regulated genes mainly involved in hormones signaling, such as ethylene, ABA, gibberellin and auxin, rather than any of the known AsA biosynthetic genes. Data mining of the corresponding<jats:italic>PVC<\/jats:italic>and<jats:italic>NVC<\/jats:italic>orthologs in Arabidopis databases identified flagellin and other ROS-producing processes as cues resulting in differential regulation of a high percentage of the genes from both groups of co-regulated genes; more specifically, 26.6% of the orthologous PVC genes, and 15.5% of the orthologous NVC genes were induced and repressed, respectively, under flagellin22 treatment in<jats:italic>Arabidopsis thaliana<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Results here reported indicate that the content of AsA in red tomato fruit from our selected RILs are not correlated with the expression of genes involved in its biosynthesis. On the contrary, the data presented here supports that AsA content in tomato fruit co-regulates with genes involved in hormone signaling and they are dependent on the oxidative status of the fruit.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1471-2164-13-187","type":"journal-article","created":{"date-parts":[[2012,5,14]],"date-time":"2012-05-14T16:17:42Z","timestamp":1337012262000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Genetic and genome-wide transcriptomic analyses identify co-regulation of oxidative response and hormone transcript abundance with vitamin C content in tomato fruit"],"prefix":"10.1186","volume":"13","author":[{"given":"Viviana","family":"Lima-Silva","sequence":"first","affiliation":[]},{"given":"Abel","family":"Rosado","sequence":"additional","affiliation":[]},{"given":"Vitor","family":"Amorim-Silva","sequence":"additional","affiliation":[]},{"given":"Antonio","family":"Mu\u00f1oz-M\u00e9rida","sequence":"additional","affiliation":[]},{"given":"Clara","family":"Pons","sequence":"additional","affiliation":[]},{"given":"Aureliano","family":"Bombarely","sequence":"additional","affiliation":[]},{"given":"Oswaldo","family":"Trelles","sequence":"additional","affiliation":[]},{"given":"Rafael","family":"Fern\u00e1ndez-Mu\u00f1oz","sequence":"additional","affiliation":[]},{"given":"Antonio","family":"Granell","sequence":"additional","affiliation":[]},{"given":"Victoriano","family":"Valpuesta","sequence":"additional","affiliation":[]},{"given":"Miguel \u00c1ngel","family":"Botella","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2012,5,14]]},"reference":[{"issue":"7","key":"4210_CR1","doi-asserted-by":"publisher","first-page":"825","DOI":"10.1002\/(SICI)1097-0010(20000515)80:7<825::AID-JSFA598>3.0.CO;2-6","volume":"80","author":"MW Davey","year":"2000","unstructured":"Davey MW, Montagu MV, Inz\u00e9 D, Sanmartin M, Kanellis A, Smirnoff N, Benzie IJJ, Strain JJ, Favell D, Fletcher J: Plant L-ascorbic acid: chemistry, function, metabolism, bioavailability and effects of processing. 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